|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Polynucleotide-Reactions → RNA-Reactions → tRNA-Reactions|
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Mass balance status: Balanced.
Enzyme Commission Primary Name: tRNA (guanine46-N7)-methyltransferase
Enzyme Commission Synonyms: Trm8/Trm82, TrmB, tRNA (m7G46) methyltransferase, transfer ribonucleate guanine 7-methyltransferase, 7-methylguanine transfer ribonucleate methylase, tRNA guanine 7-methyltransferase, N7-methylguanine methylase, S-adenosyl-L-methionine:tRNA (guanine-7-N-)-methyltransferase
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -24.765076 [Latendresse13]
Enzyme Commission Summary:
The enzyme specifically methylates guanine46 at N7 in tRNA.
Aschhoff76: Aschhoff HJ, Elten H, Arnold HH, Mahal G, Kersten W, Kersten H (1976). "7-Methylguanine specific tRNA-methyltransferase from Escherichia coli." Nucleic Acids Res 3(11);3109-22. PMID: 794833
Liu08: Liu Q, Yang W, Zhou H, Gao Y, Zhang X, Teng M, Niu L (2008). "Crystallization and preliminary crystallographic analysis of tRNA (m(7)G46) methyltransferase from Escherichia coli." Acta Crystallogr Sect F Struct Biol Cryst Commun 64(Pt 8);743-5. PMID: 18678947
Purta05: Purta E, van Vliet F, Tricot C, De Bie LG, Feder M, Skowronek K, Droogmans L, Bujnicki JM (2005). "Sequence-structure-function relationships of a tRNA (m7G46) methyltransferase studied by homology modeling and site-directed mutagenesis." Proteins 59(3);482-8. PMID: 15789416
Zegers06: Zegers I, Gigot D, van Vliet F, Tricot C, Aymerich S, Bujnicki JM, Kosinski J, Droogmans L (2006). "Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase." Nucleic Acids Res 34(6);1925-34. PMID: 16600901
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