Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 2.1.1.33

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions tRNA-Reactions

EC Number: 2.1.1.33

Enzymes and Genes:
tRNA m7G46 methyltransferase Inferred from experiment : trmI ( Escherichia coli K-12 substr. MG1655 )

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: tRNA (guanine46-N7)-methyltransferase

Enzyme Commission Synonyms: Trm8/Trm82, TrmB, tRNA (m7G46) methyltransferase, transfer ribonucleate guanine 7-methyltransferase, 7-methylguanine transfer ribonucleate methylase, tRNA guanine 7-methyltransferase, N7-methylguanine methylase, S-adenosyl-L-methionine:tRNA (guanine-7-N-)-methyltransferase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -24.765076 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The enzyme specifically methylates guanine46 at N7 in tRNA.

Citations: [Aschhoff76, Zegers06, Purta05, Liu08, Alexandrov02]

Gene-Reaction Schematic: ?

Relationship Links: BRENDA:EC:2.1.1.33 , ENZYME:EC:2.1.1.33 , IUBMB-ExplorEnz:EC:2.1.1.33


References

Alexandrov02: Alexandrov A, Martzen MR, Phizicky EM (2002). "Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA." RNA 8(10);1253-66. PMID: 12403464

Aschhoff76: Aschhoff HJ, Elten H, Arnold HH, Mahal G, Kersten W, Kersten H (1976). "7-Methylguanine specific tRNA-methyltransferase from Escherichia coli." Nucleic Acids Res 3(11);3109-22. PMID: 794833

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Liu08: Liu Q, Yang W, Zhou H, Gao Y, Zhang X, Teng M, Niu L (2008). "Crystallization and preliminary crystallographic analysis of tRNA (m(7)G46) methyltransferase from Escherichia coli." Acta Crystallogr Sect F Struct Biol Cryst Commun 64(Pt 8);743-5. PMID: 18678947

Purta05: Purta E, van Vliet F, Tricot C, De Bie LG, Feder M, Skowronek K, Droogmans L, Bujnicki JM (2005). "Sequence-structure-function relationships of a tRNA (m7G46) methyltransferase studied by homology modeling and site-directed mutagenesis." Proteins 59(3);482-8. PMID: 15789416

Zegers06: Zegers I, Gigot D, van Vliet F, Tricot C, Aymerich S, Bujnicki JM, Kosinski J, Droogmans L (2006). "Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase." Nucleic Acids Res 34(6);1925-34. PMID: 16600901


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc14.