MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions tRNA-Reactions

EC Number:

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655 : tRNA m7G46 methyltransferase Inferred from experiment : trmI

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: tRNA (guanine46-N7)-methyltransferase

Enzyme Commission Synonyms: Trm8/Trm82, TrmB, tRNA (m7G46) methyltransferase, transfer ribonucleate guanine 7-methyltransferase, 7-methylguanine transfer ribonucleate methylase, tRNA guanine 7-methyltransferase, N7-methylguanine methylase, S-adenosyl-L-methionine:tRNA (guanine-7-N-)-methyltransferase

Taxonomic Range: Fungi , Metazoa , Viridiplantae , Bacteria

Standard Gibbs Free Energy (ΔrG in kcal/mol): -24.765076 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The enzyme specifically methylates guanine46 at N7 in tRNA.

Citations: [Aschhoff76, Zegers06, Purta05, Liu08, Alexandrov02]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:


Alexandrov02: Alexandrov A, Martzen MR, Phizicky EM (2002). "Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA." RNA 8(10);1253-66. PMID: 12403464

Aschhoff76: Aschhoff HJ, Elten H, Arnold HH, Mahal G, Kersten W, Kersten H (1976). "7-Methylguanine specific tRNA-methyltransferase from Escherichia coli." Nucleic Acids Res 3(11);3109-22. PMID: 794833

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Liu08: Liu Q, Yang W, Zhou H, Gao Y, Zhang X, Teng M, Niu L (2008). "Crystallization and preliminary crystallographic analysis of tRNA (m(7)G46) methyltransferase from Escherichia coli." Acta Crystallogr Sect F Struct Biol Cryst Commun 64(Pt 8);743-5. PMID: 18678947

Purta05: Purta E, van Vliet F, Tricot C, De Bie LG, Feder M, Skowronek K, Droogmans L, Bujnicki JM (2005). "Sequence-structure-function relationships of a tRNA (m7G46) methyltransferase studied by homology modeling and site-directed mutagenesis." Proteins 59(3);482-8. PMID: 15789416

Zegers06: Zegers I, Gigot D, van Vliet F, Tricot C, Aymerich S, Bujnicki JM, Kosinski J, Droogmans L (2006). "Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase." Nucleic Acids Res 34(6);1925-34. PMID: 16600901

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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