|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 126.96.36.199
Enzymes and Genes:
xanthosine phosphorylase : xapA ( Escherichia coli K-12 substr. MG1655 )
guanosine phosphorylase ( Oryctolagus cuniculus )
purine nucleoside phosphorylase : PNP ( Homo sapiens )
purine nucleoside phosphorylase : PNP ( Plasmodium falciparum )
purine nucleoside phosphorylase : deoD ( Escherichia coli K-12 substr. MG1655 )
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Mass balance status: Balanced.
Direct generic reaction:
a purine ribonucleoside + phosphate ↔ a purine base + α-D-ribose-1-phosphate (188.8.131.52)
Enzyme Commission Primary Name: guanosine phosphorylase
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): 0.730011 [Latendresse13]
This is a specific case of the non-specific reaction catalyzed by purine-nucleoside phosphorylase a purine ribonucleoside + phosphate ↔ a purine base + α-D-ribose-1-phosphate.
Even though a different EC reaction (EC 184.108.40.206) describes this specific reaction, the E. coli enzyme has been associated with this reaction, since EC 220.127.116.11 was defined for a much more specific rabbit bone marrow enzyme [Yamada61].
Enzyme Commission Summary:
This enzyme has been defined based on a single publication of an enzyme from rabbit bone marrow that appears to be highly specific for guanosine and deoxyguanosine [Yamada61]. Most enzymes that are known to catalyze this reaction, such as the human purine nucleoside phosphorylase, can accept other purines as well, and thus were attributed to a specific case of the more general enzyme EC 18.104.22.168, (guanosine + phosphate ↔ guanine + α-D-ribose-1-phosphate).
Instance reactions of [a purine ribonucleoside + phosphate ↔ a purine base + α-D-ribose-1-phosphate] (22.214.171.124):
i1: inosine + phosphate ↔ hypoxanthine + α-D-ribose-1-phosphate (126.96.36.199)
i2: guanosine + phosphate ↔ guanine + α-D-ribose-1-phosphate (188.8.131.52)
i3: adenosine + phosphate ↔ adenine + α-D-ribose-1-phosphate (184.108.40.206)
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493