MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Homo sapiens: carnitine O-palmitoyltransferase 1, muscle isoformInferred from experiment: CPT1B
carnitine O-palmitoyltransferase 2Inferred from experiment: CPT2
carnitine O-palmitoyltransferase 1, liver isoform: CPT1A
carnitine O-palmitoyltransferase 1, brain isoform: CPT1C
Rattus norvegicus: carnitine palmitoyltransferase 1aInferred from experiment: Cpt1a
carnitine palmitoyltransferase 2Inferred from experiment: Cpt2

In Pathway: mitochondrial L-carnitine shuttle

Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .

Reaction Locations: mitochondrial lumen, cytosol

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Instance reaction:
palmitoyl-CoA + L-carnitine → L-palmitoylcarnitine + coenzyme A (

Enzyme Commission Primary Name: carnitine O-palmitoyltransferase

Enzyme Commission Synonyms: CPT, CPTo, outer malonyl-CoA inhibitable carnitine palmitoyltransferase, CPTi, CPT I (outer membrane carnitine palmitoyl transferase), carnitine palmitoyltransferase I, carnitine palmitoyltransferase II, CPT-A, CPT-B, acylcarnitine transferase, carnitine palmitoyltransferase, carnitine palmitoyltransferase-A, L-carnitine palmitoyltransferase, palmitoylcarnitine transferase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 170.99902Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Broad specificity to acyl group, over the range C8 to C18; optimal activity with palmitoyl-CoA. cf. EC carnitine O-acetyltransferase and EC carnitine O-octanoyltransferase.

Citations: [Healy88, Miyazawa83, Derrick86]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reaction of [a long-chain acyl-CoA + L-carnitine ↔ an O-acyl-L-carnitine + coenzyme A] (
i1: palmitoyl-CoA + L-carnitine → L-palmitoylcarnitine + coenzyme A (

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Derrick86: Derrick JP, Tubbs PK, Ramsay RR (1986). "Purification and properties of an easily solubilized L-carnitine palmitoyltransferase from beef-liver mitochondria." Biochem. Soc. Trans. 14 698.

Healy88: Healy MJ, Kerner J, Bieber LL (1988). "Enzymes of carnitine acylation. Is overt carnitine palmitoyltransferase of liver peroxisomal carnitine octanoyltransferase?." Biochem J 249(1);231-7. PMID: 3342008

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Miyazawa83: Miyazawa S, Ozasa H, Osumi T, Hashimoto T (1983). "Purification and properties of carnitine octanoyltransferase and carnitine palmitoyltransferase from rat liver." J Biochem 94(2);529-42. PMID: 6630173

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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