Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 3.4.21.111

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.4.21.111

Enzymes and Genes:
aqualysin I Inferred from experiment : pstI ( Thermus aquaticus YT-1 )

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Undetermined; a substrate lacks a chemical formula

Enzyme Commission Primary Name: aqualysin 1

Enzyme Commission Synonyms: caldolysin

Enzyme Commission Summary:
This enzyme from the extreme thermophile, Thermus aquaticus, is an alkaline serine peptidase. It has three subsites, S1, S2, and S3, in the substrate binding site. The preferred amino acids at the S1 site are Ala and Phe, at the S2 site are Ala and norleucine and at the S3 site are Phe and Ile. These specificities are similar to those of EC 3.4.21.62 and EC 3.4.21.64. The enzyme displays broad specificity for cleavage of insulin B-chain and hydrolyzes elastin substrates such as succinyl-(Ala)(n)-p nitroanilide (n = 1,2,3) and some peptide esters. Belongs to peptidase family S8A.

The enzyme exhibits low specificity towards esters of amino acids with small hydrophobic or aromatic residues at the P1 position.

Citations: [Tanaka98a, Matsuzawa88]

Gene-Reaction Schematic: ?

Relationship Links: BRENDA:EC:3.4.21.111 , ENZYME:EC:3.4.21.111 , IUBMB-ExplorEnz:EC:3.4.21.111


References

Matsuzawa88: Matsuzawa H, Tokugawa K, Hamaoki M, Mizoguchi M, Taguchi H, Terada I, Kwon ST, Ohta T (1988). "Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1." Eur J Biochem 171(3);441-7. PMID: 3162211

Tanaka98a: Tanaka T, Matsuzawa H, Kojima S, Kumagai I, Miura K, Ohta T (1998). "P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor." Biosci Biotechnol Biochem 62(10);2035-8. PMID: 9882104


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc13.