MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Arabidopsis thaliana col: glycerol-3-phosphate O-acyltransferaseInferred from experiment: GPAT8
cytoplasmic glycerol-3-phosphate acyltransferase: GPAT2
Escherichia coli K-12 substr. MG1655: glycerol-3-phosphate acyltransferaseInferred from experiment: plsB
Saccharomyces cerevisiae: glycerol-3-phosphate acyltransferase: GPT2
glycerol-3-phosphate 1-O-acyltransferase: SCT1

In Pathway: CDP-diacylglycerol biosynthesis I, diacylglycerol and triacylglycerol biosynthesis

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Instance reactions:
hexadecanedioyl-CoA + sn-glycerol 3-phosphate = 1-C16:0-α,ω-dicarboxyl-2-lysophosphatidate + coenzyme A (

18-hydroxyoleoyl-CoA + sn-glycerol 3-phosphate → 1-[18-hydroxyoleyl]-2-lyso-phosphatidate + coenzyme A (

Enzyme Commission Primary Name: glycerol-3-phosphate 1-O-acyltransferase

Enzyme Commission Synonyms: α-glycerophosphate acyltransferase, 3-glycerophosphate acyltransferase, ACP:sn-glycerol-3-phosphate acyltransferase, glycerol 3-phosphate acyltransferase, glycerol phosphate acyltransferase, glycerol phosphate transacylase, glycerophosphate acyltransferase, glycerophosphate transacylase, sn-glycerol 3-phosphate acyltransferase, sn-glycerol-3-phosphate acyltransferase, glycerol-3-phosphate O-acyltransferase (ambiguous)

Standard Gibbs Free Energy (ΔrG in kcal/mol): 253.16797Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Acyl-[acyl-carrier protein] can also act as acyl donor. The enzyme acts only on derivatives of fatty acids of chain length above C10.

Citations: [Bertrams81, Frentzen83, Green83, Yamashita72 ]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reactions of [a long-chain acyl-CoA + sn-glycerol 3-phosphate → a 1-acyl-sn-glycerol 3-phosphate + coenzyme A] (
i1: hexadecanedioyl-CoA + sn-glycerol 3-phosphate = 1-C16:0-α,ω-dicarboxyl-2-lysophosphatidate + coenzyme A (

i2: 18-hydroxyoleoyl-CoA + sn-glycerol 3-phosphate → 1-[18-hydroxyoleyl]-2-lyso-phosphatidate + coenzyme A (

Unification Links: KEGG:R00851, Rhea:15325

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Revised 23-Feb-2012 by Caspi R, SRI International


Bertrams81: Bertrams M, Heinz E (1981). "Positional Specificity and Fatty Acid Selectivity of Purified sn-Glycerol 3-Phosphate Acyltransferases from Chloroplasts." Plant Physiol 68(3);653-7. PMID: 16661974

Frentzen83: Frentzen M, Heinz E, McKeon TA, Stumpf PK (1983). "Specificities and selectivities of glycerol-3-phosphate acyltransferase and monoacylglycerol-3-phosphate acyltransferase from pea and spinach chloroplasts." Eur J Biochem 129(3);629-36. PMID: 6825679

Green83: Green PR, Vanaman TC, Modrich P, Bell RM (1983). "Partial NH2- and COOH-terminal sequence and cyanogen bromide peptide analysis of Escherichia coli sn-glycerol-3-phosphate acyltransferase." J Biol Chem 258(18);10862-6. PMID: 6350296

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Yamashita72: Yamashita S, Numa S (1972). "Partial purification and properties of glycerophosphate acyltransferase from rat liver. Formation of 1-acylglycerol 3-phosphate from sn-glycerol 3-phosphate and palmityl coenzyme A." Eur J Biochem 31(3);565-73. PMID: 4650158

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Fri Nov 27, 2015, BIOCYC14A.