|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 184.108.40.206
Enzymes and Genes:
|Pseudomonas putida CBB5:||methylxanthine N3-demethylase: ndmD, ndmB|
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.
Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: methylxanthine N3-demethylase
Enzyme Commission Synonyms: ndmB (gene name)
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -86.867645 [Latendresse13]
Enzyme Commission Summary:
A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida shares an NAD(P)H-FMN reductase subunit with EC 220.127.116.11, methylxanthine N1-demethylase, and has higher activity with NADH than with NADPH [Summers11]. Also demethylates caffeine and theophylline with lower efficiency. Forms part of the degradation pathway of methylxanthines.
Unification Links: KEGG:R07939
Summers11: Summers RM, Louie TM, Yu CL, Subramanian M (2011). "Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source." Microbiology 157(Pt 2);583-92. PMID: 20966097
Summers12: Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M (2012). "Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids." J Bacteriol 194(8);2041-9. PMID: 22328667
©2016 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493