|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: methylxanthine N3-demethylase
Enzyme Commission Synonyms: ndmB (gene name)
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -86.867645 [Latendresse, 2013]
Enzyme Commission Summary:
A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida shares an NAD(P)H-FMN reductase subunit with EC 22.214.171.124, methylxanthine N1-demethylase, and has higher activity with NADH than with NADPH [Summers11]. Also demethylates caffeine and theophylline with lower efficiency. Forms part of the degradation pathway of methylxanthines.
Unification Links: KEGG:R07939
Summers11: Summers RM, Louie TM, Yu CL, Subramanian M (2011). "Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source." Microbiology 157(Pt 2);583-92. PMID: 20966097
Summers12: Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M (2012). "Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids." J Bacteriol. PMID: 22328667
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