|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions → Composite Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 220.127.116.11
Enzymes and Genes:
|Methylomonas sp. 16a :||diapophytoene desaturase
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: 4,4′-diapophytoene desaturase
Enzyme Commission Synonyms: dehydrosqualene desaturase, CrtN, 4,4'-diapophytoene:FAD oxidoreductase
15-cis-4,4'-diapophytoene + FAD + H+ → all-trans-4,4'-diapophytofluene + FADH2 ,
all-trans-4,4'-diapophytofluene + FAD + H+ → all-trans-4,4'-diapo-ζ-carotene + FADH2 ,
all-trans-4,4'-diapo-ζ-carotene + FAD + H+ → all-trans-4,4'-diaponeurosporene + FADH2 ,
all-trans-4,4'-diaponeurosporene + FAD + H+ → all-trans-4,4'-diapolycopene + FADH2
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -77.26233 [Latendresse13]
Enzyme Commission Summary:
The enzyme, characterized from the bacterium Methylomonas sp. 16a, is responsible for four successive dehydrogenations. Some species possess a related enzyme that proceeds only as far as all-trans-4,4'-diaponeurosporene, cf. EC 1.3.8.o, 4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming).
Unification Links: Rhea:31394
Raisig01: Raisig A, Sandmann G (2001). "Functional properties of diapophytoene and related desaturases of C(30) and C(40) carotenoid biosynthetic pathways." Biochim Biophys Acta 1533(2);164-70. PMID: 11566453
Wieland94: Wieland B, Feil C, Gloria-Maercker E, Thumm G, Lechner M, Bravo JM, Poralla K, Gotz F (1994). "Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4'-diaponeurosporene of Staphylococcus aureus." J Bacteriol 176(24);7719-26. PMID: 8002598
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