|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 188.8.131.521
Enzymes and Genes:
|Blautia producta K 2/25.1:||NAD-dependent bile acid 3β-dehydrogenase|
|Clostridium innocuum D1144/3:||NAD-dependent bile acid 3β-dehydrogenase|
|Eggerthella lenta DSM 2243:||NAD-dependent bile acid 3β-dehydrogenase: Elen_0198|
In Pathway: glycocholate metabolism (bacteria)
Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: 3β-hydroxycholanate 3-dehydrogenase (NAD+)
Enzyme Commission Synonyms: 3β-hydroxysteroid dehydrogenase
Standard Gibbs Free Energy (ΔrG'°): 0.37820435 kcal/mol [Latendresse13]
Enzyme Commission Summary:
This bacterial enzyme is involved, along with EC 184.108.40.206, 3α-hydroxycholanate dehydrogenase, in the modification of secondary bile acids to form 3β-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. c.f. EC 220.127.116.113, 3β-hydroxysteroid dehydrogenase (NADP+).
Edenharder89: Edenharder R, Pfutzner A, Hammann R (1989). "Characterization of NAD-dependent 3 alpha- and 3 beta-hydroxysteroid dehydrogenase and of NADP-dependent 7 beta-hydroxysteroid dehydrogenase from Peptostreptococcus productus." Biochim Biophys Acta 1004(2);230-8. PMID: 2752021
Edenharder89a: Edenharder R, Pfutzner M (1989). "Partial purification and characterization of an NAD-dependent 3 beta-hydroxysteroid dehydrogenase from Clostridium innocuum." Appl Environ Microbiol 55(6);1656-9. PMID: 2764572
©2016 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493