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MetaCyc Reaction: 4.1.99.19

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 4.1.99.19

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655 : tyrosine lyase Inferred from experiment : thiH

In Pathway: thiazole biosynthesis I (E. coli)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 2-iminoacetate synthase

Enzyme Commission Synonyms: thiH (gene name)

Standard Gibbs Free Energy (ΔrG in kcal/mol): -47.847656 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Binds a [4Fe-4S] cluster that is coordinated by 3 cysteines and an exchangeable S-adenosyl-L-methionine molecule. The first stage of catalysis is reduction of the S-adenosyl-L-methionine to produce methionine and a 5-deoxyadenosin-5-yl radical that is crucial for the conversion of the substrate. Part of the pathway for thiamine biosynthesis.

Citations: [Kriek07, Leonardi03, Kriek07a]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Unification Links: Rhea:26364

Relationship Links: BRENDA:EC:4.1.99.19 , ENZYME:EC:4.1.99.19 , IUBMB-ExplorEnz:EC:4.1.99.19

Credits:
Created 15-Mar-2010 by Caspi R , SRI International


References

Kriek07: Kriek M, Martins F, Leonardi R, Fairhurst SA, Lowe DJ, Roach PL (2007). "Thiazole synthase from Escherichia coli: an investigation of the substrates and purified proteins required for activity in vitro." J Biol Chem 282(24);17413-23. PMID: 17403671

Kriek07a: Kriek M, Martins F, Challand MR, Croft A, Roach PL (2007). "Thiamine biosynthesis in Escherichia coli: identification of the intermediate and by-product derived from tyrosine." Angew Chem Int Ed Engl 46(48);9223-6. PMID: 17969213

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Leonardi03: Leonardi R, Fairhurst SA, Kriek M, Lowe DJ, Roach PL (2003). "Thiamine biosynthesis in Escherichia coli: isolation and initial characterisation of the ThiGH complex." FEBS Lett 539(1-3);95-9. PMID: 12650933


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat May 30, 2015, biocyc12.