MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateMacromolecule ReactionsPolynucleotide-ReactionsRNA-ReactionstRNA-ReactionstRNA-Charging-Reactions

EC Number:

Enzymes and Genes:

Methanocaldococcus jannaschii: O-phosphoseryl-tRNA ligaseInferred from experiment: sepS

In Pathway: L-cysteine biosynthesis II (tRNA-dependent)

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: O-phospho-L-serine—tRNA ligase

Enzyme Commission Synonyms: O-phosphoseryl-tRNA ligase, non-canonical O-phosphoseryl-tRNA synthetase, SepRS

Standard Gibbs Free Energy (ΔrG in kcal/mol): -61.770264Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
In organisms like Archaeoglobus fulgidus lacking EC (canonical cysteinyl-tRNA synthetase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which O-phosphoseryl-tRNA ligase ligates the noncanonical amino acid O-phosphoserine to tRNACys, and the Sep-tRNA:Cys-tRNA synthase converts the produced Sep-tRNACys to Cys-tRNACys, the SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [Fukunaga07]. Methanosarcina mazei can use both pathways, the canonical route using EC and the indirect pathway [Hauenstein08].

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R08576

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created 29-Jul-2009 by Caspi R, SRI International


Fukunaga07: Fukunaga R, Yokoyama S (2007). "Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea." Nat Struct Mol Biol 14(4);272-9. PMID: 17351629

Hauenstein08: Hauenstein SI, Perona JJ (2008). "Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei." J Biol Chem 283(32);22007-17. PMID: 18559341

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Fri Nov 27, 2015, BIOCYC14A.