Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014

MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:
hexulose-6-phosphate synthase Inferred from experiment : hps ( Brevibacillus brevis S1 )
hexulose-6-phosphate synthase Inferred from experiment : rmpA ( Mycobacterium gastri MN19 )
hexulose-6-phosphate synthase Inferred from experiment : rmpA ( Methylomonas aminofaciens 77a )

In Pathway: formaldehyde oxidation I , formaldehyde assimilation II (RuMP Cycle)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 3-hexulose-6-phosphate synthase

Enzyme Commission Synonyms: D-arabino-3-hexulose 6-phosphate formaldehyde-lyase, 3-hexulosephosphate synthase, 3-hexulose phosphate synthase, HPS

Standard Gibbs Free Energy (ΔrG in kcal/mol): 5.220001 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Requires Mg2+ or Mn2+ for maximal activity [Ferenci74a]. The enzyme is specific for formaldehyde and D-ribulose 5-phosphate as substrates. Ribose 5-phosphate, xylulose 5-phosphate, allulose 6-phosphate and fructose 6-phosphate cannot act as substrate. This enzyme, along with EC, 6-phospho-3-hexuloisomerase, plays a key role in the ribulose monophosphate cycle of formaldehyde fixation, which is present in many microorganisms that are capable of utilizing C1-compounds [Ferenci74a]. The hyperthermophilic and anaerobic archaeon Pyrococcus horikoshii OT3 constitutively produces a bifunctional enzyme that sequentially catalyses the reactions of this enzyme and EC, 6-phospho-3-hexuloisomerase [Orita05]. This enzyme is a member of the orotidine 5'-monophosphate decarboxylase (OMPDC) suprafamily [Kato06].

Citations: [Yurimoto05, Yanase96, Kato78]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R05338 , Rhea:25201

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:


Ferenci74a: Ferenci T, Strom T, Quayle JR (1974). "Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus." Biochem J 144(3);477-86. PMID: 4219834

Kato06: Kato N, Yurimoto H, Thauer RK (2006). "The physiological role of the ribulose monophosphate pathway in bacteria and archaea." Biosci Biotechnol Biochem 70(1);10-21. PMID: 16428816

Kato78: Kato N, Ohashi H, Tani Y, Ogata K (1978). "3-Hexulosephosphate synthase from Methylomonas aminofaciens 77a. Purification, properties and kinetics." Biochim Biophys Acta 523(1);236-44. PMID: 564713

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Orita05: Orita I, Yurimoto H, Hirai R, Kawarabayasi Y, Sakai Y, Kato N (2005). "The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathway." J Bacteriol 187(11);3636-42. PMID: 15901685

Yanase96: Yanase H, Ikeyama K, Mitsui R, Ra S, Kita K, Sakai Y, Kato N (1996). "Cloning and sequence analysis of the gene encoding 3-hexulose-6-phosphate synthase from the methylotrophic bacterium, Methylomonas aminofaciens 77a, and its expression in Escherichia coli." FEMS Microbiol Lett 135(2-3);201-5. PMID: 8595859

Yurimoto05: Yurimoto H, Kato N, Sakai Y (2005). "Assimilation, dissimilation, and detoxification of formaldehyde, a central metabolic intermediate of methylotrophic metabolism." Chem Rec 5(6);367-75. PMID: 16278835

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc14.