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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 4.1.2.43

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 4.1.2.43

Enzymes and Genes:
hexulose-6-phosphate synthase Inferred from experiment : hps ( Brevibacillus brevis S1 )
hexulose-6-phosphate synthase Inferred from experiment : rmpA ( Mycobacterium gastri MN19 )
hexulose-6-phosphate synthase Inferred from experiment : rmpA ( Methylomonas aminofaciens 77a )

In Pathway: formaldehyde oxidation I , formaldehyde assimilation II (RuMP Cycle)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 3-hexulose-6-phosphate synthase

Enzyme Commission Synonyms: D-arabino-3-hexulose 6-phosphate formaldehyde-lyase, 3-hexulosephosphate synthase, 3-hexulose phosphate synthase, HPS

Standard Gibbs Free Energy (ΔrG in kcal/mol): 5.220001 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Requires Mg2+ or Mn2+ for maximal activity [Ferenci74a]. The enzyme is specific for formaldehyde and D-ribulose 5-phosphate as substrates. Ribose 5-phosphate, xylulose 5-phosphate, allulose 6-phosphate and fructose 6-phosphate cannot act as substrate. This enzyme, along with EC 5.3.1.27, 6-phospho-3-hexuloisomerase, plays a key role in the ribulose monophosphate cycle of formaldehyde fixation, which is present in many microorganisms that are capable of utilizing C1-compounds [Ferenci74a]. The hyperthermophilic and anaerobic archaeon Pyrococcus horikoshii OT3 constitutively produces a bifunctional enzyme that sequentially catalyses the reactions of this enzyme and EC 5.3.1.27, 6-phospho-3-hexuloisomerase [Orita05]. This enzyme is a member of the orotidine 5'-monophosphate decarboxylase (OMPDC) suprafamily [Kato06].

Citations: [Yurimoto05, Yanase96, Kato78]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R05338 , Rhea:25201

Relationship Links: BRENDA:EC:4.1.2.43 , ENZYME:EC:4.1.2.43 , IUBMB-ExplorEnz:EC:4.1.2.43


References

Ferenci74a: Ferenci T, Strom T, Quayle JR (1974). "Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus." Biochem J 144(3);477-86. PMID: 4219834

Kato06: Kato N, Yurimoto H, Thauer RK (2006). "The physiological role of the ribulose monophosphate pathway in bacteria and archaea." Biosci Biotechnol Biochem 70(1);10-21. PMID: 16428816

Kato78: Kato N, Ohashi H, Tani Y, Ogata K (1978). "3-Hexulosephosphate synthase from Methylomonas aminofaciens 77a. Purification, properties and kinetics." Biochim Biophys Acta 523(1);236-44. PMID: 564713

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Orita05: Orita I, Yurimoto H, Hirai R, Kawarabayasi Y, Sakai Y, Kato N (2005). "The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathway." J Bacteriol 187(11);3636-42. PMID: 15901685

Yanase96: Yanase H, Ikeyama K, Mitsui R, Ra S, Kita K, Sakai Y, Kato N (1996). "Cloning and sequence analysis of the gene encoding 3-hexulose-6-phosphate synthase from the methylotrophic bacterium, Methylomonas aminofaciens 77a, and its expression in Escherichia coli." FEMS Microbiol Lett 135(2-3);201-5. PMID: 8595859

Yurimoto05: Yurimoto H, Kato N, Sakai Y (2005). "Assimilation, dissimilation, and detoxification of formaldehyde, a central metabolic intermediate of methylotrophic metabolism." Chem Rec 5(6);367-75. PMID: 16278835


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc14.