MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions Protein-Modification Reactions
Reactions Classified By Substrate Macromolecule Reactions Protein-Reactions Protein-Modification Reactions

EC Number:

Enzymes and Genes:

Arabidopsis thaliana col : glycine decarboxylase : AT2G35120
glycine decarboxylase : AT2G35370
Escherichia coli K-12 substr. MG1655 : aminomethyltransferase Inferred from experiment : gcvT
Gallus gallus : aminomethyltransferase Inferred from experiment : AMT
Homo sapiens : aminomethyltransferase Traceable author statement to experimental support : AMT

Sub-reaction of:
glycine + a tetrahydrofolate + NAD+ ↔ a 5,10-methylene-tetrahydrofolate + ammonium + CO2 + NADH

In Pathway: glycine cleavage

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: aminomethyltransferase

Enzyme Commission Synonyms: S-aminomethyldihydrolipoylprotein:(6S)-tetrahydrofolate aminomethyltransferase (ammonia-forming), T-protein, glycine synthase, tetrahydrofolate aminomethyltransferase, [protein]-8-S-aminomethyldihydrolipoyllysine:tetrahydrofolate aminomethyltransferase (ammonia-forming)

Standard Gibbs Free Energy (ΔrG in kcal/mol): 37.564484 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
A component, with EC, glycine dehydrogenase (decarboxylating) and EC, dihydrolipoyl dehydrogenanse, of the glycine cleavage system, formerly known as glycine synthase. The glycine cleavage system is composed of four components that only loosely associate: the P protein (EC, the T protein (EC, the L protein (EC and the lipoyl-bearing H protein [Nesbitt05].

Citations: [OkamuraIkeda82, Perham00]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reactions of [a [lipoyl-carrier protein] N6-dihydrolipoyl-L-lysine + NAD+ → a [lipoyl-carrier protein] N6-lipoyl-L-lysine + NADH + H+] (
i1: a [pyruvate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ a [pyruvate dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (

i2: a [2-oxoglutarate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ a [2-oxoglutarate dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (

i3: a [glycine-cleavage complex H protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ a [glycine-cleavage complex H protein] N6-lipoyl-L-lysine + NADH + H+ (

i4: an [apo BCAA dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ an [apo BCAA dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (

Unification Links: Rhea:16945

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC: , UniProt:RELATED-TO:O32955 , UniProt:RELATED-TO:O49954 , UniProt:RELATED-TO:P25285 , UniProt:RELATED-TO:P27248 , UniProt:RELATED-TO:P28337 , UniProt:RELATED-TO:P48728 , UniProt:RELATED-TO:P49095 , UniProt:RELATED-TO:P49361 , UniProt:RELATED-TO:P49362 , UniProt:RELATED-TO:P49363 , UniProt:RELATED-TO:P49364 , UniProt:RELATED-TO:P54260 , UniProt:RELATED-TO:P93256 , UniProt:RELATED-TO:Q9JVP2 , UniProt:RELATED-TO:Q9UZP8

Revised 18-Sep-2012 by Caspi R , SRI International


Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Nesbitt05: Nesbitt NM, Baleanu-Gogonea C, Cicchillo RM, Goodson K, Iwig DF, Broadwater JA, Haas JA, Fox BG, Booker SJ (2005). "Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase." Protein Expr Purif 39(2);269-82. PMID: 15642479

OkamuraIkeda82: Okamura-Ikeda K, Fujiwara K, Motokawa Y (1982). "Purification and characterization of chicken liver T-protein, a component of the glycine cleavage system." J Biol Chem 257(1);135-9. PMID: 7053363

Perham00: Perham RN (2000). "Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions." Annu Rev Biochem 69;961-1004. PMID: 10966480

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 13, 2015, biocyc13.