MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Bacillus subtilis : L-alanine-anticapsin ligase Inferred from experiment : bacD

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: L-amino-acid α-ligase

Enzyme Commission Synonyms: L-amino acid α-ligase, bacilysin synθse, YwfE, L-amino acid ligase

Taxonomic Range: Bacteria

Standard Gibbs Free Energy (ΔrG in kcal/mol): 13.406372 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
While the enzyme has extremely broad substrate specificity, it does not accept highly charged amino acids, such as Lys, Arg, Glu and Asp, nor does it react with secondary amines such as Pro. The N-terminal residue of the alpha-dipeptide formed seems to be limited to Ala, Gly, Ser, Thr and Met (with Ala and Ser being the most preferred), whereas the C-terminal residue seems to allow for a wider variety of amino acids (but with a preference for Met and Phe). However, not all combinations or dipeptides are formed; for example, while Ser is acceptable for the N-terminus and Thr for the C-terminus, a Ser-Thr dipeptide is not formed. D-Ala, D-Ser and D-Phe are not substrates.

Citations: [Tabata05]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Unification Links: KEGG:R07642

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:


Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Tabata05: Tabata K, Ikeda H, Hashimoto S (2005). "ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase." J Bacteriol 187(15);5195-202. PMID: 16030213

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat May 30, 2015, biocyc13.