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MetaCyc Reaction: 1.2.1.-

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions Composite Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 1.2.1.-

Enzymes and Genes:

Bacillus subtilis : branched-chain α-keto acid dehydrogenase complex Inferred from experiment : bfmBAB , bfmBAA , bfmBB , pdhD
Homo sapiens : branched-chain α-keto acid dehydrogenase complex Traceable author statement to experimental support : BCKDHB , BCKDHA , DBT , DLD

In Pathway: L-leucine degradation I

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 1.2.1 -- With NAD(+) or NADP(+) as acceptor

Sub-reactions:
4-methyl-2-oxopentanoate + an [apo BCAA dehydrogenase E2 protein] N6-lipoyl-L-lysine + H+ → a [apo BCAA dehydrogenase E2 protein] N6-S-[3-methylbutanoyl]dihydrolipoyl-L-lysine + CO2 ,
isovaleryl-CoA + an [apo BCAA dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine = a [apo BCAA dehydrogenase E2 protein] N6-S-[3-methylbutanoyl]dihydrolipoyl-L-lysine + coenzyme A ,
an [apo BCAA dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ an [apo BCAA dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+

Standard Gibbs Free Energy (ΔrG in kcal/mol): -10.194031 Inferred by computational analysis [Latendresse13]

Summary:
This reaction is the sum of three reactions.

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reactions of [a [lipoyl-carrier protein] N6-dihydrolipoyl-L-lysine + NAD+ → a [lipoyl-carrier protein] N6-lipoyl-L-lysine + NADH + H+] (1.8.1.4):
i1: an [apo BCAA dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ an [apo BCAA dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (1.8.1.4)

i2: a [glycine-cleavage complex H protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ a [glycine-cleavage complex H protein] N6-lipoyl-L-lysine + NADH + H+ (1.8.1.4)

i3: a [2-oxoglutarate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ a [2-oxoglutarate dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (1.8.1.4)

i4: a [pyruvate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+ ↔ a [pyruvate dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (1.8.1.4)

Unification Links: KEGG:R01651 , Rhea:25177


References

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu May 28, 2015, biocyc11.