|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Polynucleotide-Reactions → RNA-Reactions → tRNA-Reactions|
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: tRNA (guanosine18-2′-O)-methyltransferase
Enzyme Commission Synonyms: tRNA (Gm18) 2'-O-methyltransferase, tRNA (Gm18) methyltransferase, TrmH, SpoU
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -24.902191 [Latendresse13]
Enzyme Commission Summary:
The enzyme catalyses the methylation of guanosine18 in tRNA.
Gefter69: Gefter ML (1969). "The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli." Biochem Biophys Res Commun 36(3);435-41. PMID: 4898378
Hori98: Hori H, Yamazaki N, Matsumoto T, Watanabe Y, Ueda T, Nishikawa K, Kumagai I, Watanabe K (1998). "Substrate recognition of tRNA (Guanosine-2'-)-methyltransferase from Thermus thermophilus HB27." J Biol Chem 273(40);25721-7. PMID: 9748240
Kumagai80: Kumagai I, Watanabe K, Oshima T (1980). "Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27." Proc Natl Acad Sci U S A 77(4);1922-6. PMID: 6990416
Ochi10: Ochi A, Makabe K, Kuwajima K, Hori H (2010). "Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes." J Biol Chem 285(12);9018-29. PMID: 20053984
Pleshe05: Pleshe E, Truesdell J, Batey RT (2005). "Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus." Acta Crystallogr Sect F Struct Biol Cryst Commun 61(Pt 8);722-8. PMID: 16511140
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