MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions tRNA-Reactions

EC Number:

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655 : tRNA (Gm18) 2'-O-methyltransferase Inferred from experiment : trmH

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: tRNA (guanosine18-2′-O)-methyltransferase

Enzyme Commission Synonyms: tRNA (Gm18) 2'-O-methyltransferase, tRNA (Gm18) methyltransferase, TrmH, SpoU

Taxonomic Range: Fungi , Bacteria

Standard Gibbs Free Energy (ΔrG in kcal/mol): -24.902191 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The enzyme catalyses the methylation of guanosine18 in tRNA.

Citations: [Gefter69, Kumagai80, Hori98, Pleshe05, Ochi10]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC: , UniProt:RELATED-TO:Q9CEG9

Revised 04-Aug-2011 by Caspi R , SRI International


Gefter69: Gefter ML (1969). "The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli." Biochem Biophys Res Commun 36(3);435-41. PMID: 4898378

Hori98: Hori H, Yamazaki N, Matsumoto T, Watanabe Y, Ueda T, Nishikawa K, Kumagai I, Watanabe K (1998). "Substrate recognition of tRNA (Guanosine-2'-)-methyltransferase from Thermus thermophilus HB27." J Biol Chem 273(40);25721-7. PMID: 9748240

Kumagai80: Kumagai I, Watanabe K, Oshima T (1980). "Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27." Proc Natl Acad Sci U S A 77(4);1922-6. PMID: 6990416

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Ochi10: Ochi A, Makabe K, Kuwajima K, Hori H (2010). "Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes." J Biol Chem 285(12);9018-29. PMID: 20053984

Pleshe05: Pleshe E, Truesdell J, Batey RT (2005). "Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus." Acta Crystallogr Sect F Struct Biol Cryst Commun 61(Pt 8);722-8. PMID: 16511140

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 13, 2015, biocyc12.