Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store

MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateMacromolecule ReactionsPolynucleotide-ReactionsRNA-ReactionstRNA-Reactions

EC Number:

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655: tRNA (Gm18) 2'-O-methyltransferaseInferred from experiment: trmH

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: tRNA (guanosine18-2′-O)-methyltransferase

Enzyme Commission Synonyms: tRNA (Gm18) 2'-O-methyltransferase, tRNA (Gm18) methyltransferase, TrmH, SpoU

Taxonomic Range: Fungi, Bacteria

Standard Gibbs Free Energy (ΔrG in kcal/mol): -24.902191Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The enzyme catalyses the methylation of guanosine18 in tRNA.

Citations: [Gefter69, Kumagai80, Hori98, Pleshe05, Ochi10]

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R02917

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, UniProt:RELATED-TO:Q9CEG9

Revised 04-Aug-2011 by Caspi R, SRI International


Gefter69: Gefter ML (1969). "The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli." Biochem Biophys Res Commun 36(3);435-41. PMID: 4898378

Hori98: Hori H, Yamazaki N, Matsumoto T, Watanabe Y, Ueda T, Nishikawa K, Kumagai I, Watanabe K (1998). "Substrate recognition of tRNA (Guanosine-2'-)-methyltransferase from Thermus thermophilus HB27." J Biol Chem 273(40);25721-7. PMID: 9748240

Kumagai80: Kumagai I, Watanabe K, Oshima T (1980). "Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27." Proc Natl Acad Sci U S A 77(4);1922-6. PMID: 6990416

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Ochi10: Ochi A, Makabe K, Kuwajima K, Hori H (2010). "Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes." J Biol Chem 285(12);9018-29. PMID: 20053984

Pleshe05: Pleshe E, Truesdell J, Batey RT (2005). "Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus." Acta Crystallogr Sect F Struct Biol Cryst Commun 61(Pt 8);722-8. PMID: 16511140

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 19.5 (software by SRI International) on Tue May 3, 2016, biocyc14.