Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Reaction: 1.1.1.272

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 1.1.1.272

Enzymes and Genes:
(D)-2-hydroxyacid dehydrogenase (NADP+) Inferred from experiment : ddh ( Haloferax mediterranei )

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Instance reaction:
(R)-2-hydroxybutanoate + NADP+ = 2-oxobutanoate + NADPH + H+ (1.1.1.272)

Enzyme Commission Primary Name: D-2-hydroxyacid dehydrogenase (NADP+)

Enzyme Commission Synonyms: ddh (gene name)

Standard Gibbs Free Energy (ΔrG in kcal/mol): 22.835754 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
This enzyme, characterized from the halophilic archaeon Haloferax mediterranei, catalyses a reversible stereospecific reduction of 2-ketocarboxylic acids into the corresponding D-2-hydroxycarboxylic acids. The enzyme prefers substrates with a main chain of 5 carbons (such as 4-methyl-2-oxopentanoate) to those with a shorter chain, and can use NADH with much lower efficiency. cf. EC 1.1.1.345, (D)-2-hydroxyacid dehydrogenase (NAD+).

Citations: [Domenech06]

Gene-Reaction Schematic: ?

Instance reaction of [an (R)-2-hydroxycarboxylate + NADP+ = a 2-oxo carboxylate + NADPH + H+] (1.1.1.272):
i1: (R)-2-hydroxybutanoate + NADP+ = 2-oxobutanoate + NADPH + H+ (1.1.1.272)

Relationship Links: BRENDA:EC:1.1.1.272 , ENZYME:EC:1.1.1.272 , IUBMB-ExplorEnz:EC:1.1.1.272

Credits:
Revised 17-Sep-2012 by Caspi R , SRI International


References

Domenech06: Domenech J, Ferrer J (2006). "A new D-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression." Biochim Biophys Acta 1760(11);1667-74. PMID: 17049749

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.