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MetaCyc Polypeptide: 30S ribosomal subunit protein S21

Gene: rpsU Accession Numbers: EG10920 (MetaCyc), b3065, ECK3055

Species: Escherichia coli K-12 substr. MG1655

Component of:
30S ribosomal subunit
ribosome

Summary:
The S21 protein is a component of the 30S subunit of the ribosome. S21 was found to associate with the 50S subunit of the ribosome as well as the 30S subunit [Odom84].

Interactions between S21 and 16S rRNA have been mapped [Stern88a]. S21 also crosslinks to the 4.5S RNA of the signal recognition particle [Gu05]. Interactions between S21 and mRNA can be shown by fluorescence energy transfer [Czworkowski91] and crosslinking [Brandt92].

S21 is required for full activity of translation initiation [Held74a, Van81c]. S21 was also shown to crosslink to IF3 [MacKeen80, Cooperman81].

Processing and degradation of the rpsU-dnaG-rpoD operon mRNA has been studied; see for example [Yajnik93, Lupski84] and references therein.

Review: [Lupski84]

Locations: cytosol, ribosome

Map Position: [3,208,803 -> 3,209,018]

Unification Links: ASAP:ABE-0010063, CGSC:211, DIP:DIP-47839N, EchoBASE:EB0913, EcoGene:EG10920, EcoliWiki:b3065, Mint:MINT-1281887, OU-Microarray:b3065, PortEco:rpsU, PR:PRO_000023874, Pride:P68679, Protein Model Portal:P68679, RefSeq:NP_417537, RegulonDB:EG10920, SMR:P68679, String:511145.b3065, UniProt:P68679

Relationship Links: InterPro:IN-FAMILY:IPR001911, InterPro:IN-FAMILY:IPR018278, PDB:Structure:2YKR, PDB:Structure:3J9Y, PDB:Structure:4A2I, PDB:Structure:4ADV, PDB:Structure:4U1U, PDB:Structure:4U1V, PDB:Structure:4U20, PDB:Structure:4U24, PDB:Structure:4U25, PDB:Structure:4U26, PDB:Structure:4U27, PDB:Structure:4V4H, PDB:Structure:4V4Q, PDB:Structure:4V6O, PDB:Structure:4V6P, PDB:Structure:4V6Q, PDB:Structure:4V6R, PDB:Structure:4V6S, PDB:Structure:4V6T, PDB:Structure:4V6V, PDB:Structure:4V6Y, PDB:Structure:4V6Z, PDB:Structure:4V9O, PDB:Structure:4V9P, PDB:Structure:4V50, PDB:Structure:4V52, PDB:Structure:4V53, PDB:Structure:4V54, PDB:Structure:4V55, PDB:Structure:4V56, PDB:Structure:4V57, PDB:Structure:4V5B, PDB:Structure:4V5H, PDB:Structure:4V5Y, PDB:Structure:4V64, PDB:Structure:4V65, PDB:Structure:4V66, PDB:Structure:4V69, PDB:Structure:4V6C, PDB:Structure:4V6D, PDB:Structure:4V6E, PDB:Structure:4V6K, PDB:Structure:4V6L, PDB:Structure:4V6M, PDB:Structure:4V6N, PDB:Structure:4V70, PDB:Structure:4V71, PDB:Structure:4V72, PDB:Structure:4V73, PDB:Structure:4V74, PDB:Structure:4V75, PDB:Structure:4V76, PDB:Structure:4V77, PDB:Structure:4V78, PDB:Structure:4V79, PDB:Structure:4V7A, PDB:Structure:4V7B, PDB:Structure:4V7C, PDB:Structure:4V7D, PDB:Structure:4V7I, PDB:Structure:4V7S, PDB:Structure:4V7T, PDB:Structure:4V7U, PDB:Structure:4V7V, PDB:Structure:4V9C, PDB:Structure:4V9D, PDB:Structure:4V85, PDB:Structure:4V89, PDB:Structure:4WF1, PDB:Structure:4WWW, PDB:Structure:4YBB, PDB:Structure:5AFI, Pfam:IN-FAMILY:PF01165, Prints:IN-FAMILY:PR00976, ProDom:IN-FAMILY:PD005521, Prosite:IN-FAMILY:PS01181

Gene-Reaction Schematic

Gene-Reaction Schematic


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006412 - translation [GOA06, GOA01a]
Molecular Function:
Inferred by computational analysisGO:0003735 - structural constituent of ribosome [GOA01a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005829 - cytosol [DiazMejia09, Ishihama08]
Inferred from experimentGO:0022627 - cytosolic small ribosomal subunit [Hindennach71, WittmannLiebold73]
Inferred by computational analysisGO:0005622 - intracellular [GOA01a]
Inferred by computational analysisGO:0005840 - ribosome [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0030529 - ribonucleoprotein complex [UniProtGOA11a]

MultiFun Terms: cell structureribosomes
information transferprotein relatedribosomal proteins
information transferprotein relatedtranslation

Credits:
Last-Curated 27-Mar-2006 by Keseler I, SRI International


Subunit of: 30S ribosomal subunit

Synonyms: ribosome, small subunit

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of 30S ribosomal subunit = [RpsA][RpsB][RpsC][RpsD][RpsE][RpsF][RpsG][RpsH][RpsI][RpsJ][RpsK][RpsL][RpsM][RpsN][RpsO][RpsP][RpsQ][RpsR][RpsS][RpsT][RpsU][Sra][16S ribosomal RNA (rrsA)]
         30S ribosomal subunit protein S1 = RpsA (extended summary available)
         30S ribosomal subunit protein S2 = RpsB (summary available)
         30S ribosomal subunit protein S3 = RpsC (summary available)
         30S ribosomal subunit protein S4 = RpsD (extended summary available)
         30S ribosomal subunit protein S5 = RpsE (extended summary available)
         30S ribosomal subunit protein S6 = RpsF (extended summary available)
         30S ribosomal subunit protein S7 = RpsG (extended summary available)
         30S ribosomal subunit protein S8 = RpsH (extended summary available)
         30S ribosomal subunit protein S9 = RpsI (extended summary available)
         30S ribosomal subunit protein S10 = RpsJ (extended summary available)
         30S ribosomal subunit protein S11 = RpsK (summary available)
         30S ribosomal subunit protein S12 = RpsL (extended summary available)
         30S ribosomal subunit protein S13 = RpsM (extended summary available)
         30S ribosomal subunit protein S14 = RpsN (summary available)
         30S ribosomal subunit protein S15 = RpsO (extended summary available)
         30S ribosomal subunit protein S16 = RpsP (summary available)
         30S ribosomal subunit protein S17 = RpsQ (summary available)
         30S ribosomal subunit protein S18 = RpsR (extended summary available)
         30S ribosomal subunit protein S19 = RpsS (summary available)
         30S ribosomal subunit protein S20 = RpsT (extended summary available)
         30S ribosomal subunit protein S21 = RpsU (summary available)
         30S ribosomal subunit protein S22 = Sra (summary available)

Component of: ribosome

Enzymes activated by 30S ribosomal subunit, sorted by the type of activation, are:

Activator (Mechanism unknown) of: GTPase [Daigle04, Himeno04]

Credits:
Created 28-Mar-2006 by Keseler I, SRI International


Subunit of: ribosome

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of ribosome = [(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))][(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))]
         30S ribosomal subunit = (RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (extended summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (extended summary available)
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (extended summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (extended summary available)

Enzymes activated by ribosome, sorted by the type of activation, are:

Activator (Mechanism unknown) of: ATPase [Becker12]

Credits:
Created 15-Jun-2006 by Keseler I, SRI International


Sequence Features

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
Inferred from experiment[Vandekerckhove75]
UniProt: Removed.
Chain 2 -> 71
Author statement[UniProt15]
UniProt: 30S ribosomal protein S21.

History:
10/20/97 Gene b3065 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10920; confirmed by SwissProt match.


References

Becker12: Becker M, Gzyl KE, Altamirano AM, Vuong A, Urban K, Wieden HJ (2012). "The 70S ribosome modulates the ATPase activity of Escherichia coli YchF." RNA Biol 9(10);1288-301. PMID: 22995830

Brandt92: Brandt R, Gualerzi CO (1992). "Ribosomal localization of the mRNA in the 30S initiation complex as revealed by UV crosslinking." FEBS Lett 311(3);199-202. PMID: 1397315

Cooperman81: Cooperman BS, Expert-Bezancon A, Kahan L, Dondon J, Grunberg-Manago M (1981). "IF-3 crosslinking to Escherichia coli ribosomal 30 S subunits by three different light-dependent procedures: identification of 30 S proteins crosslinked to IF-3--utilization of a new two-stage crosslinking reagent, p-nitrobenzylmaleimide." Arch Biochem Biophys 208(2);554-62. PMID: 7020604

Czworkowski91: Czworkowski J, Odom OW, Hardesty B (1991). "Fluorescence study of the topology of messenger RNA bound to the 30S ribosomal subunit of Escherichia coli." Biochemistry 30(19);4821-30. PMID: 2029524

Daigle04: Daigle DM, Brown ED (2004). "Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro." J Bacteriol 186(5);1381-7. PMID: 14973029

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Gu05: Gu SQ, Jockel J, Beinker P, Warnecke J, Semenkov YP, Rodnina MV, Wintermeyer W (2005). "Conformation of 4.5S RNA in the signal recognition particle and on the 30S ribosomal subunit." RNA 11(9);1374-84. PMID: 16043501

Held74a: Held WA, Nomura M, Hershey JW (1974). "Ribosomal protein S21 is required for full activity in the initiation of protein synthesis." Mol Gen Genet 128(1);11-22. PMID: 4595779

Himeno04: Himeno H, Hanawa-Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, Mikami T, Odagiri F, Osanai Y, Watanabe D, Goto S, Kalachnyuk L, Ushida C, Muto A (2004). "A novel GTPase activated by the small subunit of ribosome." Nucleic Acids Res 32(17);5303-9. PMID: 15466596

Hindennach71: Hindennach I, Stoffler G, Wittmann HG (1971). "Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli." Eur J Biochem 23(1);7-11. PMID: 4942549

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Lupski84: Lupski JR, Godson GN (1984). "The rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli." Cell 39(2 Pt 1);251-2. PMID: 6388849

MacKeen80: MacKeen LA, Kahan L, Wahba AJ, Schwartz I (1980). "Photochemical cross-linking of initiation factor-3 to Escherichia coli 30 S ribosomal subunits." J Biol Chem 255(21);10526-31. PMID: 7000779

Odom84: Odom OW, Deng HY, Dabbs ER, Hardesty B (1984). "Binding of S21 to the 50S subunit and the effect of the 50S subunit on nonradiative energy transfer between the 3' end of 16S RNA and S21." Biochemistry 23(21);5069-76. PMID: 6388639

Stern88a: Stern S, Powers T, Changchien LM, Noller HF (1988). "Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNA." J Mol Biol 201(4);683-95. PMID: 2459389

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Van81c: Van Duin J, Wijnands R (1981). "The function of ribosomal protein S21 in protein synthesis." Eur J Biochem 118(3);615-9. PMID: 7028483

Vandekerckhove75: Vandekerckhove J, Rombauts W, Peeters B, Wittmann-Liebold B (1975). "Determination of the complete amino acid sequence of protein S21 from Escherichia coli ribosomes." Hoppe Seylers Z Physiol Chem 356(12);1955-76. PMID: 765257

WittmannLiebold73: Wittmann-Liebold B (1973). "Studies on the primary structure of 20 proteins from Escherichia coli ribosomes by means of an improved protein sequenator." FEBS Lett 36(3);247-9. PMID: 4587209

Yajnik93: Yajnik V, Godson GN (1993). "Selective decay of Escherichia coli dnaG messenger RNA is initiated by RNase E." J Biol Chem 268(18);13253-60. PMID: 7685758


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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