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MetaCyc Polypeptide: 50S ribosomal subunit protein L18

Gene: rplR Accession Numbers: EG10879 (MetaCyc), b3304, ECK3291

Species: Escherichia coli K-12 substr. MG1655

Component of:
50S ribosomal subunit
ribosome

Summary:
The L18 protein is a component of the 50S subunit of the ribosome and is required for incorporation of 5S rRNA into the ribosome [Rohl82].

L18 interacts with both 23S rRNA [Osswald90] and 5S rRNA [Huber84, Newberry78, Spierer78, Fanning81]. L18 together with L5 can associate into a quaternary complex with 5S and 23S rRNAs [Spierer79]. The binding determinants of the 5S rRNA-L5-L18-L25 complex have been mapped [Peattie81, Newberry80, Speek82, Brewer83, Christiansen85, Goringer86, Gewirth88, Egebjerg89, Zhang89a, Ciesiolka92, Shpanchenko96, Ostergaard98]. Iodination at a tyrosine residue abolishes binding of L18 to 5S rRNA [Fanning81a].

L18 has RNA chaperone-like activity in an in vitro trans splicing assay [Semrad04].

L18 is photoaffinity labeled by puromycin, an analog of the 3' end of aminoacylated tRNA [Bischof94]. L18 can also be crosslinked to the spiramycin derivative dihydrospiramycin and may thus be located near the peptidyl transferase center [Bischof95].

Locations: cytosol, ribosome

Map Position: [3,443,266 <- 3,443,619]

Unification Links: ASAP:ABE-0010825 , CGSC:248 , DIP:DIP-47908N , EchoBASE:EB0872 , EcoGene:EG10879 , EcoliWiki:b3304 , Mint:MINT-1293631 , ModBase:P0C018 , OU-Microarray:b3304 , PortEco:b3304 , PR:PRO_000023824 , Pride:P0C018 , Protein Model Portal:P0C018 , RefSeq:NP_417763 , RegulonDB:EG10879 , SMR:P0C018 , String:511145.b3304 , UniProt:P0C018

Relationship Links: InterPro:IN-FAMILY:IPR004389 , InterPro:IN-FAMILY:IPR005484 , PDB:Structure:1ML5 , PDB:Structure:1P85 , PDB:Structure:1P86 , PDB:Structure:1VS6 , PDB:Structure:1VS8 , PDB:Structure:1VT2 , PDB:Structure:2AW4 , PDB:Structure:2AWB , PDB:Structure:2GYA , PDB:Structure:2GYC , PDB:Structure:2I2T , PDB:Structure:2I2V , PDB:Structure:2J28 , PDB:Structure:2QAM , PDB:Structure:2QAO , PDB:Structure:2QBA , PDB:Structure:2QBC , PDB:Structure:2QBE , PDB:Structure:2QBG , PDB:Structure:2QBI , PDB:Structure:2QBK , PDB:Structure:2QOV , PDB:Structure:2QOX , PDB:Structure:2QOZ , PDB:Structure:2QP1 , PDB:Structure:2RDO , PDB:Structure:2VHM , PDB:Structure:2VHN , PDB:Structure:2WWQ , PDB:Structure:2Z4L , PDB:Structure:2Z4N , PDB:Structure:3BBX , PDB:Structure:3DF2 , PDB:Structure:3DF4 , PDB:Structure:3E1B , PDB:Structure:3E1D , PDB:Structure:3FIK , PDB:Structure:3I1N , PDB:Structure:3I1P , PDB:Structure:3I1R , PDB:Structure:3I1T , PDB:Structure:3I20 , PDB:Structure:3I22 , PDB:Structure:3IZT , PDB:Structure:3IZU , PDB:Structure:3J01 , PDB:Structure:3J0T , PDB:Structure:3J0W , PDB:Structure:3J0Y , PDB:Structure:3J11 , PDB:Structure:3J12 , PDB:Structure:3J14 , PDB:Structure:3J19 , PDB:Structure:3J37 , PDB:Structure:3KCR , PDB:Structure:3OAS , PDB:Structure:3OAT , PDB:Structure:3OFC , PDB:Structure:3OFD , PDB:Structure:3OFQ , PDB:Structure:3OFR , PDB:Structure:3OFZ , PDB:Structure:3OG0 , PDB:Structure:3ORB , PDB:Structure:3R8S , PDB:Structure:3R8T , PDB:Structure:3SGF , PDB:Structure:3UOS , PDB:Structure:4GAR , PDB:Structure:4GAU , PDB:Structure:4KIX , PDB:Structure:4KIZ , PDB:Structure:4KJ1 , PDB:Structure:4KJ3 , PDB:Structure:4KJ5 , PDB:Structure:4KJ7 , PDB:Structure:4KJ9 , PDB:Structure:4KJB , Pfam:IN-FAMILY:PF00861

Ortholog Links: Ecol199310Cyc (Escherichia coli CFT073):C4066 , EcoO157Cyc (Escherichia coli O157:H7 str. EDL933):RPLR , ShigellaCyc (Shigella flexneri 2a str. 2457T):RPLR

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006412 - translation Inferred by computational analysis [GOA06, GOA01a]
Molecular Function: GO:0003723 - RNA binding Inferred by computational analysis [GOA00]
GO:0003735 - structural constituent of ribosome Inferred by computational analysis [GOA01a]
GO:0019843 - rRNA binding Inferred by computational analysis [GOA06, GOA00]
Cellular Component: GO:0005829 - cytosol
GO:0005840 - ribosome Inferred by computational analysis [GOA01a, GOA00]
GO:0022625 - cytosolic large ribosomal subunit
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [GOA00]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation

Credits:
Last-Curated ? 24-Apr-2006 by Keseler I , SRI International


Subunit of: 50S ribosomal subunit

Synonyms: ribosome, large subunit

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of 50S ribosomal subunit = [RplA][RplB][RplC][RplD][RplE][RplF][(RplJ)([RplL]2)2][RplI][RplJ][RplK][RplM][RplN][RplO][RplP][RplQ][RplR][RplS][RplT][RplU][RplV][RplW][RplX][RplY][RpmA][RpmB][RpmC][RpmD][RpmE][RpmF][RpmG][RpmH][RpmI][RpmJ][5S ribosomal RNA (rrfA)][23S ribosomal RNA (rrlA)]
         50S ribosomal subunit protein L1 = RplA (extended summary available)
         50S ribosomal subunit protein L2 = RplB (summary available)
         50S ribosomal subunit protein L3 = RplC (summary available)
         50S ribosomal subunit protein L4 = RplD (extended summary available)
         50S ribosomal subunit protein L5 = RplE (summary available)
         50S ribosomal subunit protein L6 = RplF (summary available)
         50S ribosomal protein complex L8 = (RplJ)([RplL]2)2
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                         50S ribosomal subunit protein L12 = RplL
         50S ribosomal subunit protein L9 = RplI (summary available)
         50S ribosomal subunit protein L10 = RplJ (extended summary available)
         50S ribosomal subunit protein L11 = RplK (extended summary available)
         50S ribosomal subunit protein L13 = RplM (extended summary available)
         50S ribosomal subunit protein L14 = RplN (extended summary available)
         50S ribosomal subunit protein L15 = RplO (summary available)
         50S ribosomal subunit protein L16 = RplP (extended summary available)
         50S ribosomal subunit protein L17 = RplQ (summary available)
         50S ribosomal subunit protein L18 = RplR (extended summary available)
         50S ribosomal subunit protein L19 = RplS (extended summary available)
         50S ribosomal subunit protein L20 = RplT (extended summary available)
         50S ribosomal subunit protein L21 = RplU (summary available)
         50S ribosomal subunit protein L22 = RplV (extended summary available)
         50S ribosomal subunit protein L23 = RplW (extended summary available)
         50S ribosomal subunit protein L24 = RplX (summary available)
         50S ribosomal subunit protein L25 = RplY (summary available)
         50S ribosomal subunit protein L27 = RpmA (extended summary available)
         50S ribosomal subunit protein L28 = RpmB (summary available)
         50S ribosomal subunit protein L29 = RpmC (summary available)
         50S ribosomal subunit protein L30 = RpmD (summary available)
         50S ribosomal subunit protein L31 = RpmE (summary available)
         50S ribosomal subunit protein L32 = RpmF (summary available)
         50S ribosomal subunit protein L33 = RpmG (summary available)
         50S ribosomal subunit protein L34 = RpmH (summary available)
         50S ribosomal subunit protein L35 = RpmI (summary available)
         50S ribosomal subunit protein L36 = RpmJ (summary available)

Component of: ribosome

Credits:
Created 02-Jun-2006 by Keseler I , SRI International


Subunit of: ribosome

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of ribosome = [(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))][(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))]
         30S ribosomal subunit = (RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (summary available)
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (summary available)

Credits:
Created 15-Jun-2006 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Protein-Segment 1 -> 17
[UniProt10]
UniProt: Required for binding of the 5S rRNA/L5/L18 complex to the 23S rRNA; Sequence Annotation Type: region of interest;

History:
10/20/97 Gene b3304 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10879; confirmed by SwissProt match.


References

Bischof94: Bischof O, Kruft V, Wittmann-Liebold B (1994). "Analysis of the puromycin binding site in the 70 S ribosome of Escherichia coli at the peptide level." J Biol Chem 269(28);18315-9. PMID: 8034577

Bischof95: Bischof O, Urlaub H, Kruft V, Wittmann-Liebold B (1995). "Peptide environment of the peptidyl transferase center from Escherichia coli 70 S ribosomes as determined by thermoaffinity labeling with dihydrospiramycin." J Biol Chem 270(39);23060-4. PMID: 7559446

Brewer83: Brewer LA, Goelz S, Noller HF (1983). "Ribonucleic acid-protein cross-linking within the intact Escherichia coli ribosome, utilizing ethylene glycol bis[3-(2-ketobutyraldehyde) ether], a reversible, bifunctional reagent: synthesis and cross-linking within 30S and 50S subunits." Biochemistry 22(18);4303-9. PMID: 6354252

Christiansen85: Christiansen J, Douthwaite SR, Christensen A, Garrett RA (1985). "Does unpaired adenosine-66 from helix II of Escherichia coli 5S RNA bind to protein L18?." EMBO J 4(4);1019-24. PMID: 2990903

Ciesiolka92: Ciesiolka J, Lorenz S, Erdmann VA (1992). "Structural analysis of three prokaryotic 5S rRNA species and selected 5S rRNA--ribosomal-protein complexes by means of Pb(II)-induced hydrolysis." Eur J Biochem 204(2);575-81. PMID: 1541273

Egebjerg89: Egebjerg J, Christiansen J, Brown RS, Larsen N, Garrett RA (1989). "Protein L18 binds primarily at the junctions of helix II and internal loops A and B in Escherichia coli 5 S RNA. Implications for 5 S RNA structure." J Mol Biol 206(4);651-68. PMID: 2472486

Fanning81: Fanning TG, Traut RR (1981). "Topography of the C. coli 5S RNA-protein complex as determined by crosslinking with dimethyl suberimidate and dimethyl-3,3'-dithiobispropionimidate." Nucleic Acids Res 9(4);993-1004. PMID: 7015289

Fanning81a: Fanning TG, Traut RR (1981). "Iodination of Escherichia coli ribosomal protein L18 abolishes its 5 S RNA binding activity." Biochim Biophys Acta 652(2);256-60. PMID: 7011398

Gewirth88: Gewirth DT, Moore PB (1988). "Exploration of the L18 binding site on 5S RNA by deletion mutagenesis." Nucleic Acids Res 16(22);10717-32. PMID: 3060848

GOA00: GOA (2000). "Gene Ontology annotation based on Swiss-Prot keyword mapping."

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Goringer86: Goringer HU, Wagner R (1986). "Construction and functional analysis of ribosomal 5S RNA from Escherichia coli with single base changes in the ribosomal protein binding sites." Biol Chem Hoppe Seyler 367(8);769-80. PMID: 2429677

Huber84: Huber PW, Wool IG (1984). "Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA." Proc Natl Acad Sci U S A 81(2);322-6. PMID: 6364140

Newberry78: Newberry V, Brosius J, Garrett R (1978). "Fragment of protein L18 from the Escherichia coli ribosome that contains the 5S RNA binding site." Nucleic Acids Res 5(6);1753-66. PMID: 353728

Newberry80: Newberry V, Garrett RA (1980). "The role of the basic N-terminal region of protein L18 in 5S RNA-23S RNA complex formation." Nucleic Acids Res 8(18);4131-42. PMID: 6159586

Osswald90: Osswald M, Greuer B, Brimacombe R (1990). "Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents." Nucleic Acids Res 18(23);6755-60. PMID: 1702198

Ostergaard98: Ostergaard P, Phan H, Johansen LB, Egebjerg J, Ostergaard L, Porse BT, Garrett RA (1998). "Assembly of proteins and 5 S rRNA to transcripts of the major structural domains of 23 S rRNA." J Mol Biol 284(2);227-40. PMID: 9813114

Peattie81: Peattie DA, Douthwaite S, Garrett RA, Noller HF (1981). "A "bulged" double helix in a RNA-protein contact site." Proc Natl Acad Sci U S A 78(12);7331-5. PMID: 7038676

Rohl82: Rohl R, Nierhaus KH (1982). "Assembly map of the large subunit (50S) of Escherichia coli ribosomes." Proc Natl Acad Sci U S A 79(3);729-33. PMID: 7038683

Semrad04: Semrad K, Green R, Schroeder R (2004). "RNA chaperone activity of large ribosomal subunit proteins from Escherichia coli." RNA 10(12);1855-60. PMID: 15525706

Shpanchenko96: Shpanchenko OV, Zvereva MI, Dontsova OA, Nierhaus KH, Bogdanov AA (1996). "5S rRNA sugar-phosphate backbone protection in complexes with specific ribosomal proteins." FEBS Lett 394(1);71-5. PMID: 8925931

Speek82: Speek M, Lind A (1982). "Structural analyses of E. coli 5S RNA fragments, their associates and complexes with proteins L18 and L25." Nucleic Acids Res 10(3);947-65. PMID: 6278442

Spierer78: Spierer P, Zimmermann RA (1978). "Stoichiometry, cooperativity, and stability of interactions between 5S RNA and proteins L5, L18, and L25 from the 50S ribosomal subunit of Escherichia coli." Biochemistry 17(13);2474-9. PMID: 354687

Spierer79: Spierer P, Wang CC, Marsh TL, Zimmermann RA (1979). "Cooperative interactions among protein and RNA components of the 50S ribosomal subunit of Escherichia coli." Nucleic Acids Res 6(4);1669-82. PMID: 109811

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

Zhang89a: Zhang P, Popieniek P, Moore PB (1989). "Physical studies of 5S RNA variants at position 66." Nucleic Acids Res 17(21);8645-56. PMID: 2479908


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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, BIOCYC13B.