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MetaCyc Protein: 50S ribosomal protein complex L8

Species: Escherichia coli K-12 substr. MG1655

Component of:
50S ribosomal subunit
ribosome

Subunit composition of 50S ribosomal protein complex L8 = [RplJ][(RplL)2]2
         50S ribosomal subunit protein L10 = RplJ (extended summary available)
         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                 50S ribosomal subunit protein L12 = RplL

Gene-Reaction Schematic: ?

Credits:
Created 05-Apr-2006 by Keseler I , SRI International
Last-Curated ? 05-Apr-2006 by Keseler I , SRI International


Subunit of: 50S ribosomal subunit

Synonyms: ribosome, large subunit

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of 50S ribosomal subunit = [RplA][RplB][RplC][RplD][RplE][RplF][(RplJ)([RplL]2)2][RplI][RplJ][RplK][RplM][RplN][RplO][RplP][RplQ][RplR][RplS][RplT][RplU][RplV][RplW][RplX][RplY][RpmA][RpmB][RpmC][RpmD][RpmE][RpmF][RpmG][RpmH][RpmI][RpmJ][5S ribosomal RNA (rrfA)][23S ribosomal RNA (rrlA)]
         50S ribosomal subunit protein L1 = RplA (extended summary available)
         50S ribosomal subunit protein L2 = RplB (summary available)
         50S ribosomal subunit protein L3 = RplC (summary available)
         50S ribosomal subunit protein L4 = RplD (extended summary available)
         50S ribosomal subunit protein L5 = RplE (summary available)
         50S ribosomal subunit protein L6 = RplF (summary available)
         50S ribosomal protein complex L8 = (RplJ)([RplL]2)2
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                         50S ribosomal subunit protein L12 = RplL
         50S ribosomal subunit protein L9 = RplI (summary available)
         50S ribosomal subunit protein L10 = RplJ (extended summary available)
         50S ribosomal subunit protein L11 = RplK (extended summary available)
         50S ribosomal subunit protein L13 = RplM (extended summary available)
         50S ribosomal subunit protein L14 = RplN (extended summary available)
         50S ribosomal subunit protein L15 = RplO (summary available)
         50S ribosomal subunit protein L16 = RplP (extended summary available)
         50S ribosomal subunit protein L17 = RplQ (summary available)
         50S ribosomal subunit protein L18 = RplR (extended summary available)
         50S ribosomal subunit protein L19 = RplS (extended summary available)
         50S ribosomal subunit protein L20 = RplT (extended summary available)
         50S ribosomal subunit protein L21 = RplU (summary available)
         50S ribosomal subunit protein L22 = RplV (extended summary available)
         50S ribosomal subunit protein L23 = RplW (extended summary available)
         50S ribosomal subunit protein L24 = RplX (summary available)
         50S ribosomal subunit protein L25 = RplY (summary available)
         50S ribosomal subunit protein L27 = RpmA (extended summary available)
         50S ribosomal subunit protein L28 = RpmB (summary available)
         50S ribosomal subunit protein L29 = RpmC (summary available)
         50S ribosomal subunit protein L30 = RpmD (summary available)
         50S ribosomal subunit protein L31 = RpmE (summary available)
         50S ribosomal subunit protein L32 = RpmF (summary available)
         50S ribosomal subunit protein L33 = RpmG (summary available)
         50S ribosomal subunit protein L34 = RpmH (summary available)
         50S ribosomal subunit protein L35 = RpmI (summary available)
         50S ribosomal subunit protein L36 = RpmJ (summary available)

Component of: ribosome

Credits:
Created 02-Jun-2006 by Keseler I , SRI International


Subunit of: ribosome

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of ribosome = [(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))][(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))]
         30S ribosomal subunit = (RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (summary available)
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (summary available)

Credits:
Created 15-Jun-2006 by Keseler I , SRI International


Subunit of 50S ribosomal protein complex L8: 50S ribosomal subunit protein L10

Synonyms: RplJ

Gene: rplJ Accession Numbers: EG10871 (MetaCyc), b3985, ECK3976

Locations: cytosol, ribosome

Sequence Length: 165 AAs

GO Terms:

Biological Process: GO:0006412 - translation Inferred by computational analysis [GOA06, GOA01a]
GO:0006417 - regulation of translation Inferred by computational analysis [UniProtGOA11]
GO:0042254 - ribosome biogenesis Inferred by computational analysis [GOA01a]
Molecular Function: GO:0003723 - RNA binding Inferred by computational analysis [UniProtGOA11]
GO:0003735 - structural constituent of ribosome Inferred by computational analysis [GOA01a]
GO:0019843 - rRNA binding Inferred by computational analysis [UniProtGOA11]
GO:0070180 - large ribosomal subunit rRNA binding Inferred by computational analysis [GOA06]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, Zhang07, LopezCampistrou05]
GO:0022625 - cytosolic large ribosomal subunit Inferred from experiment [Hindennach71]
GO:0005622 - intracellular Inferred by computational analysis [GOA01a]
GO:0005840 - ribosome Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation
regulation type of regulation posttranscriptional translation attenuation and efficiency

Unification Links: DIP:DIP-35816N , EcoliWiki:b3985 , Mint:MINT-1264502 , PR:PRO_000023816 , Pride:P0A7J3 , Protein Model Portal:P0A7J3 , RefSeq:NP_418412 , SMR:P0A7J3 , String:511145.b3985 , UniProt:P0A7J3

Relationship Links: InterPro:IN-FAMILY:IPR001790 , InterPro:IN-FAMILY:IPR002363 , InterPro:IN-FAMILY:IPR022973 , Panther:IN-FAMILY:PTHR11560:SF8 , PDB:Structure:3J0T , PDB:Structure:3J0W , PDB:Structure:3J0Y , PDB:Structure:3J11 , PDB:Structure:3J12 , PDB:Structure:3J14 , PDB:Structure:3J37 , PDB:Structure:3SGF , PDB:Structure:3UOS , PDB:Structure:4KIX , PDB:Structure:4KIZ , PDB:Structure:4KJ1 , PDB:Structure:4KJ5 , PDB:Structure:4KJ9 , Pfam:IN-FAMILY:PF00466 , Prosite:IN-FAMILY:PS01109

Summary:
The L10 protein is a component of the 50S subunit of the ribosome and also regulates the expression of the L10 (β) operon at the posttranscriptional level.

L10 forms a complex with L7/L12; this complex was originally identified as the L8 subunit [Pettersson76, Chu76, Osterberg77, Gudkov78]. L10 and L7/L12 bind to the 23S rRNA early during assembly of the 50S subunit [deNarvaez79]. Binding of L10 to 23S rRNA can be separated from binding to L7/L12 [Pettersson79]; two binding sites in the C terminus of L10 are required for the binding of two L7/L12 dimers, but not for assembly of L10 into the ribosome [Griaznova00].

Synthesis of L10 and L7/L12 is regulated at the level of translation initiation [Little81, Robakis81]. Expression of L12 is closely linked to expression of L10 [Hui82], and a region within the leader sequence of the L10 operon mRNA regulates the translation efficiency of L10 and L7/L12 [Hui82, Fiil80]. The L10 protein acts at a single site in the leader mRNA [Yates81]. It has been shown that the region within the leader sequence that is essential for both translational feedback regulation and efficient translation lies between 70 and195 bases upstream of the rplJ translation start [Friesen83], and that secondary structure within that region is required for feedback regulation [Christensen84, Climie87, Climie88]. A complex containing L10 and L12 can bind directly to the leader sequence of the L10 operon mRNA and prevent translation [Johnsen82, Christensen84, Climie88], and added 23S rRNA prevents binding of L10/L12 to the mRNA [Johnsen82]. There is sequence similarity between 23S rRNA and the L10 leader region, and the L10/L12 binding site includes part of the homologous sequences [Johnsen82].

The abundance of L10 is also regulated at the post-translational level. L10 alone is subject to rapid proteolytic decay (half life of 1.5 minutes), but is stable if it is in a complex with L7/L12 [Petersen90].


Subunit of 50S ribosomal protein complex L8: 50S ribosomal subunit protein L7/L12 dimer

Gene: rplL Accession Numbers: EG10873 (MetaCyc), b3986, ECK3977

Locations: cytosol, ribosome

Subunit composition of 50S ribosomal subunit protein L7/L12 dimer = [RplL]2
         50S ribosomal subunit protein L12 = RplL

Map Position: [4,178,583 -> 4,178,948]

GO Terms:

Biological Process: GO:0006412 - translation Inferred by computational analysis [GOA06, GOA01a]
Molecular Function: GO:0003735 - structural constituent of ribosome Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, LopezCampistrou05]
GO:0022625 - cytosolic large ribosomal subunit Inferred from experiment [Hindennach71, WittmannLiebold73]
GO:0005840 - ribosome Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation

Unification Links: DIP:DIP-36009N , EcoliWiki:b3986 , Mint:MINT-1277405 , PR:PRO_000023818 , Pride:P0A7K2 , Protein Model Portal:P0A7K2 , RefSeq:NP_418413 , SMR:P0A7K2 , String:511145.b3986 , UniProt:P0A7K2

Relationship Links: InterPro:IN-FAMILY:IPR000206 , InterPro:IN-FAMILY:IPR008932 , InterPro:IN-FAMILY:IPR013823 , InterPro:IN-FAMILY:IPR014719 , PDB:Structure:1CTF , PDB:Structure:1RQS , PDB:Structure:1RQT , PDB:Structure:1RQU , PDB:Structure:1RQV , PDB:Structure:2BCW , PDB:Structure:2GYA , PDB:Structure:2GYC , PDB:Structure:2XTG , PDB:Structure:2XUX , PDB:Structure:3SGF , PDB:Structure:3UOS , PDB:Structure:4KIX , Pfam:IN-FAMILY:PF00542 , ProDom:IN-FAMILY:PD001326


References

Christensen84: Christensen T, Johnsen M, Fiil NP, Friesen JD (1984). "RNA secondary structure and translation inhibition: analysis of mutants in the rplJ leader." EMBO J 3(7);1609-12. PMID: 6378628

Chu76: Chu F, Caldwell P, Samuels M, Weissbach H, Brot N (1976). "DNA-dependent in vitro synthesis of Escherichia coli ribosomal protein L10 and the formation of an L10L12 complex." Biochem Biophys Res Commun 76(2);593-601. PMID: 800342

Climie87: Climie SC, Friesen JD (1987). "Feedback regulation of the rplJL-rpoBC ribosomal protein operon of Escherichia coli requires a region of mRNA secondary structure." J Mol Biol 198(3);371-81. PMID: 2448482

Climie88: Climie SC, Friesen JD (1988). "In vivo and in vitro structural analysis of the rplJ mRNA leader of Escherichia coli. Protection by bound L10-L7/L12." J Biol Chem 263(29);15166-75. PMID: 3049601

deNarvaez79: de Narvaez CC, Schaup HW (1979). "In vivo transcriptionally coupled assembly of Escherichia coli ribosomal subunits." J Mol Biol 134(1);1-22. PMID: 94102

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Fiil80: Fiil NP, Friesen JD, Downing WL, Dennis PP (1980). "Post-transcriptional regulatory mutants in a ribosomal protein-RNA polymerase operon of E. coli." Cell 19(4);837-44. PMID: 6991124

Friesen83: Friesen JD, Tropak M, An G (1983). "Mutations in the rpIJ leader of Escherichia coli that abolish feedback regulation." Cell 32(2);361-9. PMID: 6186394

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Griaznova00: Griaznova O, Traut RR (2000). "Deletion of C-terminal residues of Escherichia coli ribosomal protein L10 causes the loss of binding of one L7/L12 dimer: ribosomes with one L7/L12 dimer are active." Biochemistry 39(14);4075-81. PMID: 10747797

Gudkov78: Gudkov AT, Tumanova LG, Venyaminov SY, Khechinashvilli NN (1978). "Stoichiometry and properties of the complex between ribosomal proteins L7 and L10 in solution." FEBS Lett 93(2);215-8. PMID: 361431

Hindennach71: Hindennach I, Kaltschmidt E, Wittmann HG (1971). "Ribosomal proteins. Isolation of proteins from 50S ribosomal subunits of Escherichia coli." Eur J Biochem 23(1);12-6. PMID: 4942547

Hui82: Hui I, Maltman K, Little R, Hastrup S, Johnsen M, Fiil N, Dennis P (1982). "Insertions of transposon Tn5 into ribosomal protein PNA polymerase operons." J Bacteriol 152(3);1022-32. PMID: 6292159

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Johnsen82: Johnsen M, Christensen T, Dennis PP, Fiil NP (1982). "Autogenous control: ribosomal protein L10-L12 complex binds to the leader sequence of its mRNA." EMBO J 1(8);999-1004. PMID: 6765237

Little81: Little R, Fiil NP, Dennis PP (1981). "Transcriptional and post-transcriptional control of ribosomal protein and ribonucleic acid polymerase genes." J Bacteriol 147(1);25-35. PMID: 7016843

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Osterberg77: Osterberg R, Sjoberg B, Pettersson I, Liljas A, Kurland CG (1977). "Small-angle x-ray scattering study of the protein complex of L7/L12 and L10 from Escherichia coli ribosomes." FEBS Lett 73(1);22-4. PMID: 320036

Petersen90: Petersen C (1990). "Escherichia coli ribosomal protein L10 is rapidly degraded when synthesized in excess of ribosomal protein L7/L12." J Bacteriol 172(1);431-6. PMID: 2403546

Pettersson76: Pettersson I, Hardy SJ, Liljas A (1976). "The ribosomal protein L8 is a complex L7/L12 and L10." FEBS Lett 64(1);135-8. PMID: 773698

Pettersson79: Pettersson I (1979). "Studies on the RNA and protein binding sites of the E. coli ribosomal protein L10." Nucleic Acids Res 6(7);2637-46. PMID: 379826

Robakis81: Robakis N, Meza-Basso L, Brot N, Weissbach H (1981). "Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond." Proc Natl Acad Sci U S A 78(7);4261-4. PMID: 7027258

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

WittmannLiebold73: Wittmann-Liebold B (1973). "Studies on the primary structure of 20 proteins from Escherichia coli ribosomes by means of an improved protein sequenator." FEBS Lett 36(3);247-9. PMID: 4587209

Yates81: Yates JL, Dean D, Strycharz WA, Nomura M (1981). "E. coli ribosomal protein L10 inhibits translation of L10 and L7/L12 mRNAs by acting at a single site." Nature 294(5837);190-2. PMID: 6272122

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14A.