MetaCyc Pathway: melibiose degradation
Inferred from experiment

Pathway diagram: melibiose degradation

This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Superclasses: Degradation/Utilization/AssimilationCarbohydrates DegradationSugars Degradation

Some taxa known to possess this pathway include : Escherichia coli K-12 substr. MG1655

Expected Taxonomic Range: Archaea, Bacteria , Eukaryota

Escherichia coli is able to utilize melibiose as a sole source of carbon. Melibiose is imported by the melibiose permease, MelB. The melibiose is then converted to β-D-glucose and β-D-galactose by the α-galactosidase encoded by melA.

Citations: [Burstein71, Pardee57]

Unification Links: EcoCyc:PWY0-1301

Created 15-Feb-2007 by Johnson A, TIGR
Last-Curated 03-Mar-2011 by Keseler I, SRI International


Burstein71: Burstein C, Kepes A (1971). "The alpha-galactosidase from Escherichia coli K12." Biochim Biophys Acta 1971;230(1);52-63. PMID: 5543331

Pardee57: Pardee AB (1957). "An inducible mechanism for accumulation of melibiose in Escherichia coli." J Bacteriol 73(3);376-85. PMID: 13416199

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Bruel11: Bruel L, Sulzenbacher G, Cervera Tison M, Pujol A, Nicoletti C, Perrier J, Galinier A, Ropartz D, Fons M, Pompeo F, Giardina T (2011). "α-Galactosidase/sucrose kinase (AgaSK), a novel bifunctional enzyme from the human microbiome coupling galactosidase and kinase activities." J Biol Chem 286(47);40814-23. PMID: 21931163

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hanatani84: Hanatani M, Yazyu H, Shiota-Niiya S, Moriyama Y, Kanazawa H, Futai M, Tsuchiya T (1984). "Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli." J Biol Chem 259(3);1807-12. PMID: 6319412

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Legler86: Legler G, Pohl S (1986). "Synthesis of 5-amino-5-deoxy-D-galactopyranose and 1,5-dideoxy-1,5-imino-D-galactitol, and their inhibition of alpha- and beta-D-galactosidases." Carbohydr Res 155;119-29. PMID: 3024831

Liebl98: Liebl W, Wagner B, Schellhase J (1998). "Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha-and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima." Syst Appl Microbiol 1998;21(1);1-11. PMID: 9741105

Liljestrom87: Liljestrom PL, Liljestrom P (1987). "Nucleotide sequence of the melA gene, coding for alpha-galactosidase in Escherichia coli K-12." Nucleic Acids Res 15(5);2213-20. PMID: 3031590

Mandrich02: Mandrich L, Caputo E, Martin BM, Rossi M, Manco G (2002). "The Aes protein and the monomeric alpha-galactosidase from Escherichia coli form a non-covalent complex. Implications for the regulation of carbohydrate metabolism." J Biol Chem 277(50);48241-7. PMID: 12374803

Nagao88: Nagao Y, Nakada T, Imoto M, Shimamoto T, Sakai S, Tsuda M, Tsuchiya T (1988). "Purification and analysis of the structure of alpha-galactosidase from Escherichia coli." Biochem Biophys Res Commun 1988;151(1);236-41. PMID: 2831880

Pourcher90: Pourcher T, Bassilana M, Sarkar HK, Kaback HR, Leblanc G (1990). "Melibiose permease and alpha-galactosidase of Escherichia coli: identification by selective labeling using a T7 RNA polymerase/promoter expression system." Biochemistry 29(3);690-6. PMID: 2159786

Schmitt66: Schmitt R, Rotman B (1966). "Alpha-galactosidase activity in cell-free extracts of Escherichia coli." Biochem Biophys Res Commun 22(5);473-9. PMID: 5329906

Schmitt68: Schmitt R (1968). "Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12." J Bacteriol 96(2);462-71. PMID: 4877127

Shimamoto94: Shimamoto T, Noguchi K, Kuroda M, Tsuda M, Tsuchiya T (1994). "Transcriptional attenuation and differential mRNA stability in the regulation of the Escherichia coli melibiose operon." J Biochem 115(6);1185-9. PMID: 7982902

Suzuki70: Suzuki H, Li SC, Li YT (1970). "Alpha-galactosidase from Mortierella vinacea. Crystallization and properties." J Biol Chem 245(4);781-6. PMID: 5418105

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Vidershain76: Vidershain GIa, Beier EM (1976). "[Interrelation of alpha-D-fucosidase and alpha-D-galactosidase activities in man and animals]." Dokl Akad Nauk SSSR 231(2);486-8. PMID: 976079

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 20.0 (software by SRI International) on Thu May 5, 2016, BIOCYC12.