If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
|Superclasses:||Biosynthesis → Secondary Metabolites Biosynthesis → Alcohols Biosynthesis|
Expected Taxonomic Range:
The production of biofuels from renewable feedstock is a growing field, in the face of dwindling supplies and the cost of petroleum-based fuels. The primary alcohol butanol is an excellent example of a good biofuel, and is produced by several species of Clostridium. Butanol is produced by Clostridium acetobutylicum and Clostridium pasteurianum by the process of acetone-butanol-ethanol (ABE) fermentation. However, this system is hampered by productivity limitations. To circumvent these issues Clostridium pasteurianum was chemically mutated to produce copious amounts of butanol using glycerol as the sole carbon source [Malaviya12]. Glycerol is a good feedstock for butanol production because it is inexpensive and readily available, being a byproduct of biodiesel production.
About this Pathway
The butanol biosynthesis pathway from glycerol, depicted here is a naturally occurring one in Clostridium pasteurianum, albeit a system with a low yield of butanol due to the limitations of the naturally occurring ABE fermentation mechanism. Using the same pathway, Clostridium pasteurianum was chemically mutated using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to become a hyper producer of butanol.
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