This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Synonyms: PDH-bypass, ligt-dark transition acetaldehyde biosynthesis
|Superclasses:||Biosynthesis → Carbohydrates Biosynthesis|
|Biosynthesis → Secondary Metabolites Biosynthesis|
Some taxa known to possess this pathway include : Populus deltoides
Expected Taxonomic Range: Viridiplantae
Acetaldehyde is one of the products that are released by plants into the atmosphere. This compound is reactive and of immense interest to atmospheric chemists, who are not able to account for large amounts of biogenic acetaldehyde [Jardine09].
Two models that explain the biosynthesis of acetaldehyde have been proposed. According to the model depicted here, which is related to light-dark transition, acetaldehyde is produced as a correction for imbalance in the cytosolic and mitochondrial pyruvate concentrations. This pathway premises on the hypothesis that the acetaldehyde is synthesized in the leaf itself by the action of tissue localized pyruvate decarboxylase, which is usually present in the leaf veins and petioles [Nguyen09].
This pathway is similar to the (pyruvate dehydrogenase) PDH-bypass pathway for acetaldehyde that has been studied in the context of pollen development.
Jardine09: Jardine K, Karl T, Lerdau M, Harley P, Guenther A, Mak JE (2009). "Carbon isotope analysis of acetaldehyde emitted from leaves following mechanical stress and anoxia." Plant Biol (Stuttg) 11(4);591-7. PMID: 19538397
Kimmerer87: Kimmerer TW (1987). "Alcohol Dehydrogenase and Pyruvate Decarboxylase Activity in Leaves and Roots of Eastern Cottonwood (Populus deltoides Bartr.) and Soybean (Glycine max L.)." Plant Physiol 84(4);1210-1213. PMID: 16665586
Kimmerer88: Kimmerer TW, Stringer MA (1988). "Alcohol Dehydrogenase and Ethanol in the Stems of Trees : Evidence for Anaerobic Metabolism in the Vascular Cambium." Plant Physiol 87(3);693-697. PMID: 16666209
Nguyen09: Nguyen T, Drotar AM, Monson RK, Fall R (2009). "A high affinity pyruvate decarboxylase is present in cottonwood leaf veins and petioles: a second source of leaf acetaldehyde emission?." Phytochemistry 70(10);1217-21. PMID: 19698964
Catalanotti12: Catalanotti C, Dubini A, Subramanian V, Yang W, Magneschi L, Mus F, Seibert M, Posewitz MC, Grossman AR (2012). "Altered fermentative metabolism in Chlamydomonas reinhardtii mutants lacking pyruvate formate lyase and both pyruvate formate lyase and alcohol dehydrogenase." Plant Cell 24(2);692-707. PMID: 22353371
Chae, 2011: Chae, Lee (2011). "The functional annotation of protein sequences was performed by the in-house Ensemble Enzyme Prediction Pipeline (E2P2, version 1.0). E2P2 systematically integrates results from three molecular function annotation algorithms using an ensemble classification scheme. For a given genome, all protein sequences are submitted as individual queries against the base-level annotation methods. The individual methods rely on homology transfer to annotate protein sequences, using single sequence (BLAST, E-value cutoff <= 1e-30, subset of SwissProt 15.3) and multiple sequence (Priam, November 2010; CatFam, version 2.0, 1% FDR profile library) models of enzymatic functions. The base-level predictions are then integrated into a final set of annotations using an average weighted integration algorithm, where the weight of each prediction from each individual method was determined via a 0.632 bootstrap process over 1000 rounds of testing. The training and testing data for E2P2 and the BLAST reference database were drawn from protein sequences with experimental support of existence, compiled from SwissProt release 15.3."
Dickinson00a: Dickinson JR, Harrison SJ, Dickinson JA, Hewlins MJ (2000). "An investigation of the metabolism of isoleucine to active Amyl alcohol in Saccharomyces cerevisiae." J Biol Chem 275(15);10937-42. PMID: 10753893
Flikweert99: Flikweert MT, de Swaaf M, van Dijken JP, Pronk JT (1999). "Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae." FEMS Microbiol Lett 174(1);73-9. PMID: 10234824
Hemschemeier08: Hemschemeier A, Jacobs J, Happe T (2008). "Biochemical and physiological characterization of the pyruvate formate-lyase Pfl1 of Chlamydomonas reinhardtii, a typically bacterial enzyme in a eukaryotic alga." Eukaryot Cell 7(3);518-26. PMID: 18245276
KillenbergJabs96: Killenberg-Jabs M, Konig S, Hohmann S, Hubner G (1996). "Purification and characterisation of the pyruvate decarboxylase from a haploid strain of Saccharomyces cerevisiae." Biol Chem Hoppe Seyler 377(5);313-7. PMID: 8828822
Lee85a: Lee T.C., Langston-Unkefer P.J. "Pyruvate decarboxylase from Zea mays L. I. Purification and partial characterization from mature kernels and anaerobically treated roots." Plant Physiol. (1985) 79:242-247.
Mucke96: Mucke U, Wohlfarth T, Fiedler U, Baumlein H, Rucknagel KP, Konig S (1996). "Pyruvate decarboxylase from Pisum sativum. Properties, nucleotide and amino acid sequences." Eur J Biochem 237(2);373-82. PMID: 8647075
Neale87: Neale AD, Scopes RK, Wettenhall RE, Hoogenraad NJ (1987). "Pyruvate decarboxylase of Zymomonas mobilis: isolation, properties, and genetic expression in Escherichia coli." J Bacteriol 169(3);1024-8. PMID: 3546263
Sirikantaramas09: Sirikantaramas S, Yamazaki M, Saito K (2009). "A survival strategy: The coevolution of the camptothecin biosynthetic pathway and self-resistance mechanism." Phytochemistry. PMID: 19709698
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