This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Synonyms: dalcochinin glucoside hydrolysis, dalbergia isoflavonoid β-glucoside hydrolysis
|Superclasses:||Biosynthesis → Secondary Metabolites Biosynthesis → Phenylpropanoid Derivatives Biosynthesis → Flavonoids Biosynthesis → Isoflavonoids Biosynthesis|
Some taxa known to possess this pathway include : Dalbergia cochinchinensis
Expected Taxonomic Range: Fabaceae
The isoflavonoid glycosides in legumes have conserved patterns of glycosylations, the monosaccharides and dissacharides are usually β-glucopyranosides. Dissacharides among the isoflavonoid glucosides are known to occur less frequently. Nevertheless, unusual isoflavone glycosides are reported every year, for example the uncommon sugar β-apiofuranose has been found a s a monosaccharid invariably O-linked to C-4' of the isoflavone aglycone or as a terminal sugar of various disaccharids [Vietch07].
The biosynthesis of legume isoflavonoid glycosides is poorly understood. A few isoflavonoid-specific glucotransferases have been cloned providing some insight into the mechanism of isoflavonoid production in legumes. In Dalbergia cochinchinensis, an isoflavonoid β-glucosidase called dalcochinase had been isolated and cloned [Chuankhayan07]. This enzyme hydrolyzes the rotenoid glycoside dalcochinin-8'-O-β-glucoside [Toonkool06]. Dalcochinase has also been shown to carry out reverse hydrolysis of saccharides in the presence of excess sugars.
Chuankhayan07: Chuankhayan P, Rimlumduan T, Tantanuch W, Mothong N, Kongsaeree PT, Metheenukul P, Svasti J, Jensen ON, Cairns JR (2007). "Functional and structural differences between isoflavonoid beta-glycosidases from Dalbergia sp." Arch Biochem Biophys 468(2);205-16. PMID: 17998137
Toonkool06: Toonkool P, Metheenukul P, Sujiwattanarat P, Paiboon P, Tongtubtim N, Ketudat-Cairns M, Ketudat-Cairns J, Svasti J (2006). "Expression and purification of dalcochinase, a beta-glucosidase from Dalbergia cochinchinensis Pierre, in yeast and bacterial hosts." Protein Expr Purif 48(2);195-204. PMID: 16814564
Ketudat00: Ketudat Cairns JR, Champattanachai V, Srisomsap C, Wittman-Liebold B, Thiede B, Svasti J (2000). "Sequence and expression of Thai Rosewood beta-glucosidase/beta-fucosidase, a family 1 glycosyl hydrolase glycoprotein." J Biochem 128(6);999-1008. PMID: 11098143
Srisomsap96: Srisomsap C, Svasti J, Surarit R, Champattanachai V, Sawangareetrakul P, Boonpuan K, Subhasitanont P, Chokchaichamnankit D (1996). "Isolation and characterization of an enzyme with beta-glucosidase and beta-fucosidase activities from Dalbergia cochinchinensis Pierre." J Biochem 119(3);585-90. PMID: 8830058
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