If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Synonyms: ECA biosynthesis
|Superclasses:||Biosynthesis → Cell Structures Biosynthesis|
Some taxa known to possess this pathway include : Escherichia coli K-12 substr. MG1655
Expected Taxonomic Range: Proteobacteria
Enterobacterial common antigen (ECA) is an outer membrane glycolipid common to all members of Enterobacteriaceae. The carbohydrate portion consists of N-acetyl-glucosamine, N-acetyl-D-mannosaminuronic acid and 4-acetamido-4,6-dideoxy-D-galactose. These amino sugars form trisaccharide repeat units which are part of linear heteropolysaccharide chains [MeierDieter90].
Unification Links: EcoCyc:ECASYN-PWY
Danese98: Danese PN, Oliver GR, Barr K, Bowman GD, Rick PD, Silhavy TJ (1998). "Accumulation of the enterobacterial common antigen lipid II biosynthetic intermediate stimulates degP transcription in Escherichia coli." J Bacteriol 1998;180(22);5875-84. PMID: 9811644
MeierDieter90: Meier-Dieter U, Starman R, Barr K, Mayer H, Rick PD (1990). "Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis." J Biol Chem 1990;265(23);13490-7. PMID: 2166030
Aguirrezabalaga00: Aguirrezabalaga I, Olano C, Allende N, Rodriguez L, Brana AF, Mendez C, Salas JA (2000). "Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus." Antimicrob Agents Chemother 44(5);1266-75. PMID: 10770761
AlDabbagh08: Al-Dabbagh B, Mengin-Lecreulx D, Bouhss A (2008). "Purification and characterization of the bacterial UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase WecA." J Bacteriol 190(21);7141-6. PMID: 18723618
AlDabbagh09: Al-Dabbagh B, Blanot D, Mengin-Lecreulx D, Bouhss A (2009). "Preparative enzymatic synthesis of polyprenyl-pyrophosphoryl-N-acetylglucosamine, an essential lipid intermediate for the biosynthesis of various bacterial cell envelope polymers." Anal Biochem 391(2);163-5. PMID: 19442646
Amer00: Amer AO, Valvano MA (2000). "The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion." J Bacteriol 182(2);498-503. PMID: 10629198
Amer01: Amer AO, Valvano MA (2001). "Conserved amino acid residues found in a predicted cytosolic domain of the lipopolysaccharide biosynthetic protein WecA are implicated in the recognition of UDP-N-acetylglucosamine." Microbiology 147(Pt 11);3015-25. PMID: 11700352
Amer02: Amer AO, Valvano MA (2002). "Conserved aspartic acids are essential for the enzymic activity of the WecA protein initiating the biosynthesis of O-specific lipopolysaccharide and enterobacterial common antigen in Escherichia coli." Microbiology 148(Pt 2);571-82. PMID: 11832520
Barr88: Barr K, Ward S, Meier-Dieter U, Mayer H, Rick PD (1988). "Characterization of an Escherichia coli rff mutant defective in transfer of N-acetylmannosaminuronic acid (ManNAcA) from UDP-ManNAcA to a lipid-linked intermediate involved in enterobacterial common antigen synthesis." J Bacteriol 1988;170(1);228-33. PMID: 3275612
Campbell00: Campbell RE, Mosimann SC, Tanner ME, Strynadka NC (2000). "The structure of UDP-N-acetylglucosamine 2-epimerase reveals homology to phosphoglycosyl transferases." Biochemistry 39(49);14993-5001. PMID: 11106477
Chen09: Chen YL, Chen YH, Lin YC, Tsai KC, Chiu HT (2009). "Functional characterization and substrate specificity of spinosyn rhamnosyltransferase by in vitro reconstitution of spinosyn biosynthetic enzymes." J Biol Chem 284(11);7352-63. PMID: 19126547
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Erbel03: Erbel PJ, Barr K, Gao N, Gerwig GJ, Rick PD, Gardner KH (2003). "Identification and biosynthesis of cyclic enterobacterial common antigen in Escherichia coli." J Bacteriol 185(6);1995-2004. PMID: 12618464
Furlong12: Furlong SE, Valvano MA (2012). "Characterization of the highly conserved VFMGD motif in a bacterial polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase." Protein Sci 21(9);1366-75. PMID: 22811320
Gabriel68: Gabriel O, Lindquist LC (1968). "Biological mechanisms involved in the formation of deoxy sugars. IV. Enzymatic conversion of thymidine diphosphoglucose-4T to thymidine diphospho-4-keto-6-deoxyglucose-6T." J Biol Chem 1968;243(7);1479-84. PMID: 4869561
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