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MetaCyc Pathway: enterobacterial common antigen biosynthesis
Inferred from experiment

Pathway diagram: enterobacterial common antigen biosynthesis

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Synonyms: ECA biosynthesis

Superclasses: BiosynthesisCell Structures Biosynthesis

Some taxa known to possess this pathway include : Escherichia coli K-12 substr. MG1655

Expected Taxonomic Range: Proteobacteria

Enterobacterial common antigen (ECA) is an outer membrane glycolipid common to all members of Enterobacteriaceae. The carbohydrate portion consists of N-acetyl-glucosamine, N-acetyl-D-mannosaminuronic acid and 4-acetamido-4,6-dideoxy-D-galactose. These amino sugars form trisaccharide repeat units which are part of linear heteropolysaccharide chains [MeierDieter90].

Subpathways: UDP-N-acetyl-α-D-mannosaminouronate biosynthesis, dTDP-N-acetylthomosamine biosynthesis

Unification Links: EcoCyc:ECASYN-PWY


Danese98: Danese PN, Oliver GR, Barr K, Bowman GD, Rick PD, Silhavy TJ (1998). "Accumulation of the enterobacterial common antigen lipid II biosynthetic intermediate stimulates degP transcription in Escherichia coli." J Bacteriol 1998;180(22);5875-84. PMID: 9811644

MeierDieter90: Meier-Dieter U, Starman R, Barr K, Mayer H, Rick PD (1990). "Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis." J Biol Chem 1990;265(23);13490-7. PMID: 2166030

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Aguirrezabalaga00: Aguirrezabalaga I, Olano C, Allende N, Rodriguez L, Brana AF, Mendez C, Salas JA (2000). "Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus." Antimicrob Agents Chemother 44(5);1266-75. PMID: 10770761

AlDabbagh08: Al-Dabbagh B, Mengin-Lecreulx D, Bouhss A (2008). "Purification and characterization of the bacterial UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase WecA." J Bacteriol 190(21);7141-6. PMID: 18723618

AlDabbagh09: Al-Dabbagh B, Blanot D, Mengin-Lecreulx D, Bouhss A (2009). "Preparative enzymatic synthesis of polyprenyl-pyrophosphoryl-N-acetylglucosamine, an essential lipid intermediate for the biosynthesis of various bacterial cell envelope polymers." Anal Biochem 391(2);163-5. PMID: 19442646

Allard04: Allard ST, Cleland WW, Holden HM (2004). "High resolution X-ray structure of dTDP-glucose 4,6-dehydratase from Streptomyces venezuelae." J Biol Chem 279(3);2211-20. PMID: 14570895

Amer00: Amer AO, Valvano MA (2000). "The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion." J Bacteriol 182(2);498-503. PMID: 10629198

Amer01: Amer AO, Valvano MA (2001). "Conserved amino acid residues found in a predicted cytosolic domain of the lipopolysaccharide biosynthetic protein WecA are implicated in the recognition of UDP-N-acetylglucosamine." Microbiology 147(Pt 11);3015-25. PMID: 11700352

Amer02: Amer AO, Valvano MA (2002). "Conserved aspartic acids are essential for the enzymic activity of the WecA protein initiating the biosynthesis of O-specific lipopolysaccharide and enterobacterial common antigen in Escherichia coli." Microbiology 148(Pt 2);571-82. PMID: 11832520

Bairoch93: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529

Barr87: Barr K, Rick PD (1987). "Biosynthesis of enterobacterial common antigen in Escherichia coli. In vitro synthesis of lipid-linked intermediates." J Biol Chem 1987;262(15);7142-50. PMID: 3034883

Barr88: Barr K, Ward S, Meier-Dieter U, Mayer H, Rick PD (1988). "Characterization of an Escherichia coli rff mutant defective in transfer of N-acetylmannosaminuronic acid (ManNAcA) from UDP-ManNAcA to a lipid-linked intermediate involved in enterobacterial common antigen synthesis." J Bacteriol 1988;170(1);228-33. PMID: 3275612

Barr93: Barr K, Rick PD (1993). "Physical map location of the rffC and rffA genes of Escherichia coli." J Bacteriol 175(17);5738-9. PMID: 8366065

Bernstein65: Bernstein R, Robbins P "Control aspects of uridine 5'-diphosphate glucose and thymidine 5'-diphosphate glucose synthesis by microbial enzymes." J Biol Chem 1965;240(1):391-397.

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Burrows00: Burrows LL, Pigeon KE, Lam JS (2000). "Pseudomonas aeruginosa B-band lipopolysaccharide genes wbpA and wbpI and their Escherichia coli homologues wecC and wecB are not functionally interchangeable." FEMS Microbiol Lett 189(2);135-41. PMID: 10930727

Campbell00: Campbell RE, Mosimann SC, Tanner ME, Strynadka NC (2000). "The structure of UDP-N-acetylglucosamine 2-epimerase reveals homology to phosphoglycosyl transferases." Biochemistry 39(49);14993-5001. PMID: 11106477

Chen09: Chen YL, Chen YH, Lin YC, Tsai KC, Chiu HT (2009). "Functional characterization and substrate specificity of spinosyn rhamnosyltransferase by in vitro reconstitution of spinosyn biosynthetic enzymes." J Biol Chem 284(11);7352-63. PMID: 19126547

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Erbel03: Erbel PJ, Barr K, Gao N, Gerwig GJ, Rick PD, Gardner KH (2003). "Identification and biosynthesis of cyclic enterobacterial common antigen in Escherichia coli." J Bacteriol 185(6);1995-2004. PMID: 12618464

Furlong12: Furlong SE, Valvano MA (2012). "Characterization of the highly conserved VFMGD motif in a bacterial polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase." Protein Sci 21(9);1366-75. PMID: 22811320

Showing only 20 references. To show more, press the button "Show all references".

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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