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MetaCyc Enzyme: 2-pyrone-4,6-dicarboxylate hydrolase

Gene: pmdD Accession Number: G-2543 (MetaCyc)

Species: Comamonas testosteroni

Summary:
2-pyrone-4,6-dicarboxylate hydrolase was purified from 4-hydroxybenzoate-grown Comamonas testosteroni. The molecular weight was 42.8 kD by gel filtration and 37.2 kD by SDS-PAGE [Kersten82]. Inhibition studies suggested that the hydrolase might possess a functioning sulfhydryl group at its catalytic site. The reaction is reversible.

Gene Citations: [Providenti01]

Molecular Weight of Polypeptide: 34.451 kD (from nucleotide sequence), 37.2 kD (experimental) [Kersten82 ]

Unification Links: ModBase:Q93PS7 , Protein Model Portal:Q93PS7 , SMR:Q93PS7 , Swiss-Model:Q93PS7 , UniProt:Q93PS7

Relationship Links: Entrez-Nucleotide:RELATED-TO:AF305325 , InterPro:IN-FAMILY:IPR006992 , Pfam:IN-FAMILY:PF04909

Gene-Reaction Schematic: ?

Gene-Reaction Schematic


Enzymatic reaction of: 2-pyrone-4,6-dicarboxylate hydrolase

EC Number: 3.1.1.57

2-pyrone-4,6-dicarboxylate + H2O <=> (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible. [Kersten82]

In Pathways: protocatechuate degradation I (meta-cleavage pathway) , methylgallate degradation

Inhibitors (Unknown Mechanism): Zn2+ [Kersten82] , Cu2+ [Kersten82] , Mn2+ [Kersten82] , Co2+ [Kersten82]

Kinetic Parameters:

Substrate
Km (μM)
Citations
2-pyrone-4,6-dicarboxylate
90.0
[Kersten82]

pH(opt): 8.6 [Kersten82]


References

Kersten82: Kersten PJ, Dagley S, Whittaker JW, Arciero DM, Lipscomb JD (1982). "2-pyrone-4,6-dicarboxylic acid, a catabolite of gallic acids in Pseudomonas species." J Bacteriol 1982;152(3);1154-62. PMID: 7142106

Providenti01: Providenti MA, Mampel J, MacSween S, Cook AM, Wyndham RC (2001). "Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate." Microbiology 147(Pt 8);2157-67. PMID: 11495993


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sun Mar 29, 2015, biocyc13.