|Gene:||pmdD||Accession Number: G-2543 (MetaCyc)|
Species: Comamonas testosteroni
2-pyrone-4,6-dicarboxylate hydrolase was purified from 4-hydroxybenzoate-grown Comamonas testosteroni. The molecular weight was 42.8 kD by gel filtration and 37.2 kD by SDS-PAGE [Kersten82]. Inhibition studies suggested that the hydrolase might possess a functioning sulfhydryl group at its catalytic site. The reaction is reversible.
Gene Citations: [Providenti01]
Molecular Weight of Polypeptide: 34.451 kD (from nucleotide sequence), 37.2 kD (experimental) [Kersten82 ]
Enzymatic reaction of: 2-pyrone-4,6-dicarboxylate hydrolase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
This reaction is reversible. [Kersten82]
pH(opt): 8.6 [Kersten82]
Kersten82: Kersten PJ, Dagley S, Whittaker JW, Arciero DM, Lipscomb JD (1982). "2-pyrone-4,6-dicarboxylic acid, a catabolite of gallic acids in Pseudomonas species." J Bacteriol 1982;152(3);1154-62. PMID: 7142106
Providenti01: Providenti MA, Mampel J, MacSween S, Cook AM, Wyndham RC (2001). "Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate." Microbiology 147(Pt 8);2157-67. PMID: 11495993
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