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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: 2-pyrone-4,6-dicarboxylate hydrolase

Gene: pmdD Accession Number: G-2543 (MetaCyc)

Species: Comamonas testosteroni

Summary:
2-pyrone-4,6-dicarboxylate hydrolase was purified from 4-hydroxybenzoate-grown Comamonas testosteroni. The molecular weight was 42.8 kD by gel filtration and 37.2 kD by SDS-PAGE [Kersten82]. Inhibition studies suggested that the hydrolase might possess a functioning sulfhydryl group at its catalytic site. The reaction is reversible.

Gene Citations: [Providenti01]

Molecular Weight of Polypeptide: 34.451 kD (from nucleotide sequence), 37.2 kD (experimental) [Kersten82 ]

Unification Links: ModBase:Q93PS7 , Protein Model Portal:Q93PS7 , SMR:Q93PS7 , Swiss-Model:Q93PS7 , UniProt:Q93PS7

Relationship Links: Entrez-Nucleotide:RELATED-TO:AF305325 , InterPro:IN-FAMILY:IPR006992 , Pfam:IN-FAMILY:PF04909

Gene-Reaction Schematic: ?


Enzymatic reaction of: 2-pyrone-4,6-dicarboxylate hydrolase

EC Number: 3.1.1.57

2-pyrone-4,6-dicarboxylate + H2O <=> (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Kersten82]

In Pathways: protocatechuate degradation I (meta-cleavage pathway) , methylgallate degradation

Inhibitors (Unknown Mechanism): Zn2+ [Kersten82] , Cu2+ [Kersten82] , Mn2+ [Kersten82] , Co2+ [Kersten82]

Kinetic Parameters:

Substrate
Km (μM)
Citations
2-pyrone-4,6-dicarboxylate
90.0
[Kersten82]

pH(opt): 8.6 [Kersten82]


References

Kersten82: Kersten PJ, Dagley S, Whittaker JW, Arciero DM, Lipscomb JD (1982). "2-pyrone-4,6-dicarboxylic acid, a catabolite of gallic acids in Pseudomonas species." J Bacteriol 1982;152(3);1154-62. PMID: 7142106

Providenti01: Providenti MA, Mampel J, MacSween S, Cook AM, Wyndham RC (2001). "Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate." Microbiology 147(Pt 8);2157-67. PMID: 11495993


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, BIOCYC14A.