Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015

MetaCyc Enzyme: flavin-dependent monooxygenase RebC

Gene: rebC Accession Number: G-11522 (MetaCyc)

Synonyms: rbmD

Species: Lechevalieria aerocolonigenes

RebC is a monomeric enzyme with three distinct domains. It binds a FAD cofactor, and has been characterized as a flavin-dependent monooxygenase [Ryan07]. The protein requires NADH for FAD reduction [HowardJones06].

The rebC gene was cloned into Escherichia coli and the protein was expressed as a His-tag fusion and purified [HowardJones06].

Upon flavin reduction, the RebC crystal undergoes a change in its unit cell dimension concurrent with a 5Å movement of the isoalloxazine ring, positioning the flavin ring adjacent to the substrate-binding pocket [Ryan08].

Gene Citations: [Sanchez02]

Molecular Weight of Polypeptide: 57.671 kD (from nucleotide sequence), 59.0 kD (experimental) [HowardJones06 ]

Unification Links: Protein Model Portal:Q8KI25 , SMR:Q8KI25 , UniProt:Q8KI25

Relationship Links: Entrez-Nucleotide:PART-OF:AF534707 , InterPro:IN-FAMILY:IPR002938 , InterPro:IN-FAMILY:IPR003042 , PDB:Structure:2R0C , PDB:Structure:2R0G , PDB:Structure:2R0P , PDB:Structure:3EPT , PDB:Structure:4EIP , PDB:Structure:4EIQ , Pfam:IN-FAMILY:PF01494 , Prints:IN-FAMILY:PR00420

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 09-Sep-2009 by Caspi R , SRI International

Enzymatic reaction of: flavin dependent hydroxylase (flavin-dependent monooxygenase RebC)

EC Number:

dichlorochromopyrrolate + 4 NADH + 4 oxygen + 6 H+ <=> dichloro-arcyriaflavin A + 2 CO2 + 4 NAD+ + 6 H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: rebeccamycin biosynthesis

Cofactors or Prosthetic Groups: FAD [HowardJones06]

Kinetic Parameters:

Km (μM)


HowardJones06: Howard-Jones AR, Walsh CT (2006). "Staurosporine and rebeccamycin aglycones are assembled by the oxidative action of StaP, StaC, and RebC on chromopyrrolic acid." J Am Chem Soc 128(37);12289-98. PMID: 16967980

Ryan07: Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL (2007). "Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC." Proc Natl Acad Sci U S A 104(39);15311-6. PMID: 17873060

Ryan08: Ryan KS, Chakraborty S, Howard-Jones AR, Walsh CT, Ballou DP, Drennan CL (2008). "The FAD cofactor of RebC shifts to an IN conformation upon flavin reduction." Biochemistry 47(51);13506-13. PMID: 19035832

Sanchez02: Sanchez C, Butovich IA, Brana AF, Rohr J, Mendez C, Salas JA (2002). "The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives." Chem Biol 9(4);519-31. PMID: 11983340

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Jun 30, 2015, BIOCYC14A.