|Gene:||rebC||Accession Number: G-11522 (MetaCyc)|
Species: Lechevalieria aerocolonigenes
RebC is a monomeric enzyme with three distinct domains. It binds a FAD cofactor, and has been characterized as a flavin-dependent monooxygenase [Ryan07]. The protein requires NADH for FAD reduction [HowardJones06].
Upon flavin reduction, the RebC crystal undergoes a change in its unit cell dimension concurrent with a 5Å movement of the isoalloxazine ring, positioning the flavin ring adjacent to the substrate-binding pocket [Ryan08].
Gene Citations: [Sanchez02]
Molecular Weight of Polypeptide: 57.671 kD (from nucleotide sequence), 59.0 kD (experimental) [HowardJones06 ]
Relationship Links: Entrez-Nucleotide:PART-OF:AF534707 , InterPro:IN-FAMILY:IPR002938 , InterPro:IN-FAMILY:IPR003042 , PDB:Structure:2R0C , PDB:Structure:2R0G , PDB:Structure:2R0P , PDB:Structure:3EPT , PDB:Structure:4EIP , PDB:Structure:4EIQ , Pfam:IN-FAMILY:PF01494 , Prints:IN-FAMILY:PR00420
Enzymatic reaction of: flavin dependent hydroxylase (flavin-dependent monooxygenase RebC)
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
In Pathways: rebeccamycin biosynthesis
HowardJones06: Howard-Jones AR, Walsh CT (2006). "Staurosporine and rebeccamycin aglycones are assembled by the oxidative action of StaP, StaC, and RebC on chromopyrrolic acid." J Am Chem Soc 128(37);12289-98. PMID: 16967980
Ryan07: Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL (2007). "Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC." Proc Natl Acad Sci U S A 104(39);15311-6. PMID: 17873060
Ryan08: Ryan KS, Chakraborty S, Howard-Jones AR, Walsh CT, Ballou DP, Drennan CL (2008). "The FAD cofactor of RebC shifts to an IN conformation upon flavin reduction." Biochemistry 47(51);13506-13. PMID: 19035832
Sanchez02: Sanchez C, Butovich IA, Brana AF, Rohr J, Mendez C, Salas JA (2002). "The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives." Chem Biol 9(4);519-31. PMID: 11983340
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