|Gene:||yeiC||Accession Number: G-10824 (MetaCyc)|
Species: Escherichia coli UTI89
yeiC was the most overexpressed gene when the uropathogenic strain Escherichia coli UTI89 was grown on human urine [Snyder04a]. The gene was cloned, expressed as a fusion protein with a His-tag, and purified to homogeneity [Preumont08].
The enzymatic activity was determined by measuring enzymatically the formation of ADP in the presence of different nucleosides and ATP. The enzyme converted ATP to ADP in the presence of pseudouridine, but not in the presence of uridine, adenosine, guanosine or cytidine, indicating that it was specific for pseudouridine.
The enzyme was dependent on the presence of Mg2+. Its Km for pseudouridine was 120 μM in the presence of 0.5 mM ATP-Mg and its Km for ATP-Mg was 330 μM in the presence of 200 μM pseudouridine [Preumont08].
Molecular Weight of Polypeptide: 38.13 kD (from nucleotide sequence)
Enzymatic reaction of: pseudouridine kinase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: pseudouridine degradation
Snyder04a: Snyder JA, Haugen BJ, Buckles EL, Lockatell CV, Johnson DE, Donnenberg MS, Welch RA, Mobley HL (2004). "Transcriptome of uropathogenic Escherichia coli during urinary tract infection." Infect Immun 72(11);6373-81. PMID: 15501767
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