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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: pseudouridine kinase

Gene: yeiC Accession Number: G-10824 (MetaCyc)

Species: Escherichia coli UTI89

Summary:
yeiC was the most overexpressed gene when the uropathogenic strain Escherichia coli UTI89 was grown on human urine [Snyder04a]. The gene was cloned, expressed as a fusion protein with a His-tag, and purified to homogeneity [Preumont08].

The enzymatic activity was determined by measuring enzymatically the formation of ADP in the presence of different nucleosides and ATP. The enzyme converted ATP to ADP in the presence of pseudouridine, but not in the presence of uridine, adenosine, guanosine or cytidine, indicating that it was specific for pseudouridine.

The enzyme was dependent on the presence of Mg2+. Its Km for pseudouridine was 120 μM in the presence of 0.5 mM ATP-Mg and its Km for ATP-Mg was 330 μM in the presence of 200 μM pseudouridine [Preumont08].

Molecular Weight of Polypeptide: 38.13 kD (from nucleotide sequence)

Unification Links: Entrez-gene:3990814 , Protein Model Portal:Q1R9Q6 , String:364106.UTI89_C2440 , UniProt:Q1R9Q6

Relationship Links: InterPro:IN-FAMILY:IPR002173 , InterPro:IN-FAMILY:IPR011611 , Pfam:IN-FAMILY:PF00294 , Prosite:IN-FAMILY:PS00583 , Prosite:IN-FAMILY:PS00584

Gene-Reaction Schematic: ?

Credits:
Created 27-Aug-2008 by Caspi R , SRI International


Enzymatic reaction of: pseudouridine kinase

EC Number: 2.7.1.83

pseudouridine + ATP <=> pseudouridine 5'-phosphate + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: pseudouridine degradation

Activators (Allosteric): Mg2+ [Preumont08]

Kinetic Parameters:

Substrate
Km (μM)
Citations
pseudouridine
120.0
[Preumont08]
ATP
330.0
[Preumont08]


References

Preumont08: Preumont A, Snoussi K, Stroobant V, Collet JF, Van Schaftingen E (2008). "Molecular identification of pseudouridine metabolizing enzymes." J Biol Chem 283(37):25238-46. PMID: 18591240

Snyder04a: Snyder JA, Haugen BJ, Buckles EL, Lockatell CV, Johnson DE, Donnenberg MS, Welch RA, Mobley HL (2004). "Transcriptome of uropathogenic Escherichia coli during urinary tract infection." Infect Immun 72(11);6373-81. PMID: 15501767


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14A.