|Gene:||cofF||Accession Number: G-9428 (MetaCyc)|
Species: Methanocaldococcus jannaschii
The cofF (MJ1001) gene of Methanocaldococcus jannaschii, a homolog of the Escherichia coli K-12 rimK gene, was cloned and expressed in Escherichia coli. The resulting monomeric protein was shown to be a γ-F420-2:α-l-glutamate ligase, the enzyme that caps the γ-glutamyl tail of the hydride carrier coenzyme F420 [Li03a].
Molecular Weight of Polypeptide: 33.112 kD (from nucleotide sequence), 37 kD (experimental) [Li03a ]
Relationship Links: Entrez-Nucleotide:RELATED-TO:L77117 , InterPro:IN-FAMILY:IPR004666 , InterPro:IN-FAMILY:IPR011761 , InterPro:IN-FAMILY:IPR013651 , InterPro:IN-FAMILY:IPR013815 , InterPro:IN-FAMILY:IPR013816 , Pfam:IN-FAMILY:PF08443 , Prosite:IN-FAMILY:PS50975
Enzymatic reaction of: γ-F420-2:α-L-glutamate ligase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
Vmax was 0.72 nmol/min/mg protein [Li03a].
Li03a: Li H, Xu H, Graham DE, White RH (2003). "Glutathione synthetase homologs encode alpha-L-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses." Proc Natl Acad Sci U S A 100(17);9785-90. PMID: 12909715
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