Synonyms: nicotinate hydroxylase
Species: Pseudomonas fluorescens TN5
It has been shown that the oxygen of the hydroxyl group is derived from water, rather than molecular oxygen. The identity of the physiological electron acceptor in P. fluorescens is not known, but there is evidence that nicotinate dehydrogenase is linked to the cytochrome respiratory chain [Hunt58].
The molecular mass of 80 kDa was determined by SDS-PAGE. Gel-permeation HPLC gave a molecular mass of 100 kD [Hurh94].
Molecular Weight of Polypeptide: 80 kD (experimental) [Hurh94]
Enzymatic reaction of: nicotinate dehydrogenase
EC Number: 18.104.22.168nicotinate + 2 an oxidized cytochrome + H2O → 6-hydroxynicotinate + 2 a reduced cytochrome + 2 H+
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.Alternative Substrates for nicotinate: 3-pyridinesulfonate [Hurh94]
In Pathways: nicotinate degradation IInhibitors (Unknown Mechanism): Hg2+ [Hurh94], Cu2+ [Hurh94], Ag+ [Hurh94], p-chloromercuribenzoate [Hurh94], N-ethylmaleimide [Hurh94]Kinetic Parameters:
T(opt): 50 °C [Hurh94]
pH(opt): 8.3 [Hurh94]
©2016 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493