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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: nicotinate dehydrogenase (cytochrome)

Synonyms: nicotinate hydroxylase

Species: Pseudomonas fluorescens TN5

Summary:
It has been shown that the oxygen of the hydroxyl group is derived from water, rather than molecular oxygen. The identity of the physiological electron acceptor in P. fluorescens is not known, but there is evidence that nicotinate dehydrogenase is linked to the cytochrome respiratory chain [Hunt58].

The molecular mass of 80 kDa was determined by SDS-PAGE. Gel-permeation HPLC gave a molecular mass of 100 kD [Hurh94].

Locations: membrane

Molecular Weight of Polypeptide: 80 kD (experimental) [Hurh94 ]

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0016020 - membrane [Hurh94]


Enzymatic reaction of: nicotinate dehydrogenase

EC Number: 1.17.2.1

nicotinate + an oxidized cytochrome + H2O <=> 6-hydroxynicotinate + a reduced cytochrome + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for nicotinate: 3-pyridinesulfonate [Hurh94 ]

In Pathways: nicotinate degradation I

Inhibitors (Unknown Mechanism): Hg2+ [Hurh94] , Cu2+ [Hurh94] , Ag+ [Hurh94] , p-chloromercuribenzoate [Hurh94] , N-ethylmaleimide [Hurh94]

Kinetic Parameters:

Substrate
Km (μM)
Citations
nicotinate
110.0
[Hurh94]

T(opt): 50 °C [Hurh94]

pH(opt): 8.3 [Hurh94]


References

Hunt58: Hunt, A.L., Hughes, D.E., Lowenstein, J.M. (1958). "The hydroxylation of nicotinic acid by Pseudomonas fluorescens." Biochem J 69(2);170-3. PMID: 13546162

Hurh94: Hurh B, Yamane T, Nagasawa T (1994). "Purification and characterization of nicotinic acid dehydrogenase from Pseudomonas fluorescens TN5." J. Ferment. Bioeng. 78, No. 1, 19-26.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc11.