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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: sarcosine oxidase

Gene: soxA Accession Number: G-8522 (MetaCyc)

Species: Arthrobacter sp. TE1826

Summary:
The sarcosine oxidase from Arthrobacter sp. TE1826 is encoded by the soxA gene, a part of a creatinine degradation operon, which also contains the genes for creatininase (crnA) and creatinase (creA), a regulator (soxR) and two putative transporters [Nishiya98].

The protein was purified from its native host, and the gene encoding it was cloned and expressed in Escherichia coli and Bacillus subtilis. While the protein is produced in its native host only in the presence of sarcosine, the heterologous hosts produce it constitutively, indicating that a regulatory region that is recognized by Arthrobacter is not recognized in the otehr organisms [Nishiya93].

The enzyme was later subjected to site-directed mutagenesis in an attempt to alter its substrate specificity and optimum pH [Nishiya94]. Five different mutants in position 103 (originally phenylalanine) were generated, with striking differences in substrate specificity [Nishiya94].

Locations: cytosol

Molecular Weight of Polypeptide: 43.249 kD (from nucleotide sequence), 43 kD (experimental) [Nishiya93 ]

Unification Links: ModBase:P40873 , Protein Model Portal:P40873 , SMR:P40873 , Swiss-Model:P40873 , UniProt:P40873

Relationship Links: Entrez-Nucleotide:RELATED-TO:AB007122 , InterPro:IN-FAMILY:IPR006076 , InterPro:IN-FAMILY:IPR006281 , Pfam:IN-FAMILY:PF01266

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005829 - cytosol [Nishiya93]

MultiFun Terms: metabolism degradation of macromolecules proteins/peptides/glycopeptides


Enzymatic reaction of: sarcosine oxidase

EC Number: 1.5.3.1

sarcosine + oxygen + H2O <=> glycine + formaldehyde + hydrogen peroxide

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: creatinine degradation I

Cofactors or Prosthetic Groups: FAD [Nishiya93]

Inhibitors (Unknown Mechanism): Ag+ [Nishiya93] , Hg2+ [Nishiya93] , p-chloromercuribenzoate [Nishiya93]

Kinetic Parameters:

Substrate
Km (μM)
Citations
sarcosine
3600.0
[Nishiya94]

pH(opt): 7.5 [Nishiya94]


References

Nishiya93: Nishiya, Y., Imanaka, T. (1993). "Cloning and sequencing of the sarcosine oxidase gene from Arthrobacter sp. TE1826." J. Ferment. bioeng. 75 (4):239-244.

Nishiya94: Nishiya, Y., Imanaka, T. (1994). "Alteration of substrate specificity and optimum pH of sarcosine oxidase by random and site-directed mutagenesis." Appl. and Environ. Mocrobiol. 60 (11):4213-4215.

Nishiya98: Nishiya Y, Toda A, Imanaka T (1998). "Gene cluster for creatinine degradation in Arthrobacter sp. TE1826." Mol Gen Genet 257(5);581-6. PMID: 9563845


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, BIOCYC13A.