|Gene:||soxA||Accession Number: G-8522 (MetaCyc)|
Species: Arthrobacter sp. TE1826
The sarcosine oxidase from Arthrobacter sp. TE1826 is encoded by the soxA gene, a part of a creatinine degradation operon, which also contains the genes for creatininase (crnA) and creatinase (creA), a regulator (soxR) and two putative transporters [Nishiya98].
The protein was purified from its native host, and the gene encoding it was cloned and expressed in Escherichia coli and Bacillus subtilis. While the protein is produced in its native host only in the presence of sarcosine, the heterologous hosts produce it constitutively, indicating that a regulatory region that is recognized by Arthrobacter is not recognized in the otehr organisms [Nishiya93].
The enzyme was later subjected to site-directed mutagenesis in an attempt to alter its substrate specificity and optimum pH [Nishiya94]. Five different mutants in position 103 (originally phenylalanine) were generated, with striking differences in substrate specificity [Nishiya94].
Molecular Weight of Polypeptide: 43.249 kD (from nucleotide sequence), 43 kD (experimental) [Nishiya93 ]
|Cellular Component:||GO:0005829 - cytosol [Nishiya93]|
|MultiFun Terms:||metabolism → degradation of macromolecules → proteins/peptides/glycopeptides|
Enzymatic reaction of: sarcosine oxidase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
In Pathways: creatinine degradation I
pH(opt): 7.5 [Nishiya94]
Nishiya94: Nishiya, Y., Imanaka, T. (1994). "Alteration of substrate specificity and optimum pH of sarcosine oxidase by random and site-directed mutagenesis." Appl. and Environ. Mocrobiol. 60 (11):4213-4215.
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