|Gene:||soxA||Accession Number: G-8522 (MetaCyc)|
Species: Arthrobacter sp. TE1826
The sarcosine oxidase from Arthrobacter sp. TE1826 is encoded by the soxA gene, a part of a creatinine degradation operon, which also contains the genes for creatininase (crnA) and creatinase (creA), a regulator (soxR) and two putative transporters [Nishiya98].
The protein was purified from its native host, and the gene encoding it was cloned and expressed in Escherichia coli and Bacillus subtilis. While the protein is produced in its native host only in the presence of sarcosine, the heterologous hosts produce it constitutively, indicating that a regulatory region that is recognized by Arthrobacter is not recognized in the otehr organisms [Nishiya93].
The enzyme was later subjected to site-directed mutagenesis in an attempt to alter its substrate specificity and optimum pH [Nishiya94]. Five different mutants in position 103 (originally phenylalanine) were generated, with striking differences in substrate specificity [Nishiya94].
Molecular Weight of Polypeptide: 43.249 kD (from nucleotide sequence), 43 kD (experimental) [Nishiya93 ]
|Substrate||References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway||>||onMouseOver="return overlib(' Nishiya, Y., Imanaka, T. (1994). "Alteration of substrate specificity and optimum pH of sarcosine oxidase by random and site-directed mutagenesis." Appl. and Environ. Mocrobiol. 60 (11):4213-4215.', WIDTH, 500);" onmouseout="return nd();">Nishiya94onMouseOver="return overlib('24516157', WIDTH, 68);" onmouseout="return nd();">Blank14: : : 7.5 Burton08: [ Burton RL, Hanes JW, Grant GA (2008). "A stopped flow transient kinetic analysis of substrate binding and catalysis in Escherichia coli D-3-phosphoglycerate dehydrogenase." J Biol Chem 283(44);29706-14. PMID: 18776184 onMouseOver="return overlib('7642604', WIDTH, 62);" onmouseout="return nd();"> Nishiya93: onMouseOver="return overlib('10858298', WIDTH, 68);" onmouseout="return nd();"> Contestabile00 Nishiya, Y., Imanaka, T. (1993). "Cloning and sequencing of the sarcosine oxidase gene from : Arthrobacter sp. TE1826." J. Ferment. bioeng. 75 (4):239-244. Nishiya94: Nishiya Y, Toda A, Imanaka T (1998). "Gene cluster for creatinine degradation in Arthrobacter sp. TE1826." Mol Gen Genet 257(5);581-6. PMID: 9563845 Dubrow77: Dubrow R, Pizer LI (1977). "Transient kinetic studies on the allosteric transition of phosphoglycerate dehydrogenase." J Biol Chem 1977;252(5);1527-38. PMID: 320209 Duncan86: Duncan K, Coggins JR (1986). "The serC-aro A operon of Escherichia coli. A mixed function operon encoding enzymes from two different amino acid biosynthetic pathways." Biochem J 1986;234(1);49-57. PMID: 3518706 ECOSAL: "Escherichia coli and Salmonella: Cellular and Molecular Biology." Online edition. Florio09: Florio R, Chiaraluce R, Consalvi V, Paiardini A, Catacchio B, Bossa F, Contestabile R (2009). "The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase." FEBS J 276(1);132-43. PMID: 19019081 Florio09a: Florio R, Chiaraluce R, Consalvi V, Paiardini A, Catacchio B, Bossa F, Contestabile R (2009). "Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase--the role of evolutionarily conserved hydrophobic contacts." FEBS J 276(24);7319-28. PMID: 19909338 Herrington13: Herrington MB, Sitaras C (2013). "The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12." Arch Microbiol 195(8);559-69. PMID: 23824318 Hopkins86: Hopkins S, Schirch V (1986). "Properties of a serine hydroxymethyltransferase in which an active site histidine has been changed to an asparagine by site-directed mutagenesis." J Biol Chem 1986;261(7);3363-9. PMID: 3512553 JoshiTope90: Joshi-Tope G, Schirch V (1990). "The role of a critical sulfhydryl group in the mechanism of serine hydroxymethyltransferase." Ann N Y Acad Sci 585;339-45. PMID: 2113366 Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394 Kuznetsova06: Kuznetsova E, Proudfoot M, Gonzalez CF, Brown G, Omelchenko MV, Borozan I, Carmel L, Wolf YI, Mori H, Savchenko AV, Arrowsmith CH, Koonin EV, Edwards AM, Yakunin AF (2006). "Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family." J Biol Chem 281(47):36149-61. PMID: 16990279 [Bairoch93: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529 pH(opt) Blank D, Wolf L, Ackermann M, Silander OK (2014). "The predictability of molecular evolution during functional innovation." Proc Natl Acad Sci U S A 111(8);3044-9. PMID: 24516157 Cai95: References Cai K, Schirch D, Schirch V (1995). "The affinity of pyridoxal 5'-phosphate for folding intermediates of Escherichia coli serine hydroxymethyltransferase." J Biol Chem 270(33);19294-9. PMID: 7642604 Cai K, Schirch D, Schirch V (1995). "The affinity of pyridoxal 5'-phosphate for folding intermediates of Escherichia coli serine hydroxymethyltransferase." J Biol Chem 270(33);19294-9. PMID: 7642604 Contestabile R, Angelaccio S, Bossa F, Wright HT, Scarsdale N, Kazanina G, Schirch V (2000). "Role of tyrosine 65 in the mechanism of serine hydroxymethyltransferase." Biochemistry 39(25);7492-500. PMID: 10858298 Delle94: Nishiya98 Delle Fratte S, Iurescia S, Angelaccio S, Bossa F, Schirch V (1994). "The function of arginine 363 as the substrate carboxyl-binding site in Escherichia coli serine hydroxymethyltransferase." Eur J Biochem 225(1);395-401. PMID: 7925461|
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