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MetaCyc Enzyme: cis-geranyl-CoA carboxylase

Species: Pseudomonas aeruginosa PAO1

Subunit composition of cis-geranyl-CoA carboxylase = [AtuF][AtuC]
         cis-geranyl-CoA carboxylase α-subunit = AtuF (summary available)
         cis-geranyl-CoA carboxylase β-subunit = AtuC (summary available)

Summary:
The subunit coefficients of the complex have not been reported.

This enzyme is a biotin-containing, biotin-dependent carboxylase involved in the acyclic terpene utilization pathway of Pseudomonas aeruginosa PAO1 (including pathways citronellol degradation, cis-genanyl-CoA degradation and leucine degradation I). The enzyme is expressed in citronellol or citronellate grown cells, but is not expressed in isovalerate grown cells (that utilize pathway leucine degradation I). Genes atuC and atuF encode the two subunits of cis-geranyl-CoA carboxylase. The subunits are encoded in the atuABCDEFGH gene cluster of this organism ([Hoschle05] and reviewed in [ForsterFromme10]).

The enzyme was purified from cell extracts by avidin-affinity chromatography and the SDS-gel-isolated subunits were subjected to trypsin fingerprint analysis and ESI-MS which allowed identification of their corresponding genes [Hoschle05].

Recombinant enzyme has been expressed in Escherichia coli, purified and subjected to kinetic analysis [Aguilar08].

The cis-geranyl-CoA carboxylase of Pseudomonas citronellolis was characterized in earlier work [Fall81, Fall77, Hector76] and the acyclic terpene utilization gene cluster in this organism has been identified and characterized [ForsterFromme06].

Gene-Reaction Schematic: ?

Credits:
Created 14-Dec-2010 by Fulcher CA , SRI International


Enzymatic reaction of: cis-geranyl-CoA carboxylase

EC Number: 6.4.1.5

cis-geranyl-CoA + hydrogen carbonate + ATP <=> 3-(4-methylpent-3-en-1-yl)-pent-2-enedioyl-CoA + ADP + phosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for cis-geranyl-CoA: 3-methylcrotonyl-CoA [Aguilar08 ]

In Pathways: cis-genanyl-CoA degradation

Summary:
This enzyme catalyzes the condensation of cis-geranyl-CoA and hydrogen carbonate to form 3-(4-methylpent-3-en-1-yl)-pent-2-enedioyl-CoA (isohexenyl-glutaconyl-CoA). Purified, recombinant enzyme showed sigmoidal kinetics for cis-geranyl-CoA as substrate, but Michaelis-Menten kinetics for 3-methylcrotonyl-CoA and ATP as substrates [Aguilar08].

Cofactors or Prosthetic Groups: biotin [Aguilar08]

T(opt): 37 °C [Aguilar08]

pH(opt): 8.5 [Aguilar08]


Subunit of cis-geranyl-CoA carboxylase: cis-geranyl-CoA carboxylase α-subunit

Synonyms: AtuF, geranyl-CoA carboxylase, alpha-subunit (biotin-containing)

Gene: atuF Accession Number: G-12379 (MetaCyc)

Sequence Length: 661 AAs

Molecular Weight: 71.748 kD (from nucleotide sequence)

Molecular Weight: 74.0 kD (experimental) [Aguilar08]

Unification Links: Protein Model Portal:Q9HZV6 , SMR:Q9HZV6 , String:208964.PA2891 , UniProt:Q9HZV6

Relationship Links: InterPro:IN-FAMILY:IPR000089 , InterPro:IN-FAMILY:IPR001882 , InterPro:IN-FAMILY:IPR005479 , InterPro:IN-FAMILY:IPR005481 , InterPro:IN-FAMILY:IPR005482 , InterPro:IN-FAMILY:IPR011053 , InterPro:IN-FAMILY:IPR011054 , InterPro:IN-FAMILY:IPR011761 , InterPro:IN-FAMILY:IPR011764 , InterPro:IN-FAMILY:IPR013815 , InterPro:IN-FAMILY:IPR013816 , InterPro:IN-FAMILY:IPR016185 , Pfam:IN-FAMILY:PF00289 , Pfam:IN-FAMILY:PF00364 , Pfam:IN-FAMILY:PF02785 , Pfam:IN-FAMILY:PF02786 , Prosite:IN-FAMILY:PS00188 , Prosite:IN-FAMILY:PS00866 , Prosite:IN-FAMILY:PS00867 , Prosite:IN-FAMILY:PS50968 , Prosite:IN-FAMILY:PS50975 , Prosite:IN-FAMILY:PS50979 , Smart:IN-FAMILY:SM00878

Summary:
The subunit apparent molecular mass was determined by SDS-PAGE [Aguilar08].


Subunit of cis-geranyl-CoA carboxylase: cis-geranyl-CoA carboxylase β-subunit

Synonyms: AtuC

Gene: atuC Accession Number: G-12378 (MetaCyc)

Sequence Length: 538 AAs

Molecular Weight: 57.329 kD (from nucleotide sequence)

Molecular Weight: 63.0 kD (experimental) [Aguilar08]

Unification Links: Protein Model Portal:Q9HZV9 , SMR:Q9HZV9 , String:208964.PA2888 , UniProt:Q9HZV9

Relationship Links: InterPro:IN-FAMILY:IPR000022 , InterPro:IN-FAMILY:IPR011762 , InterPro:IN-FAMILY:IPR011763 , Pfam:IN-FAMILY:PF01039 , Prosite:IN-FAMILY:PS50980 , Prosite:IN-FAMILY:PS50989

Summary:
The subunit apparent molecular mass was determined by SDS-PAGE [Aguilar08].


References

Aguilar08: Aguilar JA, Diaz-Perez C, Diaz-Perez AL, Rodriguez-Zavala JS, Nikolau BJ, Campos-Garcia J (2008). "Substrate specificity of the 3-methylcrotonyl coenzyme A (CoA) and geranyl-CoA carboxylases from Pseudomonas aeruginosa." J Bacteriol 190(14);4888-93. PMID: 18469096

Demyttenaere04: Demyttenaere JC, Vanoverschelde J, De Kimpe N (2004). "Biotransformation of (R)-(+)- and (S)-(-)-citronellol by Aspergillus sp. and Penicillium sp., and the use of solid-phase microextraction for screening." J Chromatogr A 1027(1-2);137-46. PMID: 14971495

Fall77: Fall RR, Hector ML (1977). "Acyl-coenzyme A carboxylases. Homologous 3-methylcrotonyl-CoA and geranyl-CoA carboxylases from Pseudomonas citronellolis." Biochemistry 16(18);4000-5. PMID: 911753

Fall81: Fall RR (1981). "3-Methylcrotonyl-CoA and geranyl-CoA carboxylases from Pseudomonas citronellolis." Methods Enzymol 71 Pt C;791-9. PMID: 7278686

ForsterFromme06: Forster-Fromme K, Jendrossek D (2006). "Identification and characterization of the acyclic terpene utilization gene cluster of Pseudomonas citronellolis." FEMS Microbiol Lett 264(2);220-5. PMID: 17064376

ForsterFromme10: Forster-Fromme K, Jendrossek D (2010). "Catabolism of citronellol and related acyclic terpenoids in pseudomonads." Appl Microbiol Biotechnol 87(3);859-69. PMID: 20490788

Hector76: Hector ML, Fall RR (1976). "Multiple acyl-coenzyme A carboxylases in Pseudomonas citronellolis." Biochemistry 15(16);3465-72. PMID: 8091

Hoschle05: Hoschle B, Gnau V, Jendrossek D (2005). "Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa." Microbiology 151(Pt 11);3649-56. PMID: 16272386


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc11.