MetaCyc Enzyme: cis-geranyl-CoA carboxylase

Species: Pseudomonas aeruginosa PAO1

Subunit composition of cis-geranyl-CoA carboxylase = [AtuF][AtuC]
         cis-geranyl-CoA carboxylase α-subunit = AtuF (summary available)
         cis-geranyl-CoA carboxylase β-subunit = AtuC (summary available)

The subunit coefficients of the complex have not been reported.

This enzyme is a biotin-containing, biotin-dependent carboxylase involved in the acyclic terpene utilization pathway of Pseudomonas aeruginosa PAO1 (including pathways citronellol degradation, cis-genanyl-CoA degradation and L-leucine degradation I). The enzyme is expressed in citronellol or citronellate grown cells, but is not expressed in isovalerate grown cells (that utilize pathway L-leucine degradation I). Genes atuC and atuF encode the two subunits of cis-geranyl-CoA carboxylase. The subunits are encoded in the atuABCDEFGH gene cluster of this organism ([Hoschle05] and reviewed in [ForsterFromme10]).

The enzyme was purified from cell extracts by avidin-affinity chromatography and the SDS-gel-isolated subunits were subjected to trypsin fingerprint analysis and ESI-MS which allowed identification of their corresponding genes [Hoschle05].

Recombinant enzyme has been expressed in Escherichia coli, purified and subjected to kinetic analysis [Aguilar08].

The cis-geranyl-CoA carboxylase of Pseudomonas citronellolis was characterized in earlier work [Fall81, Fall77, Hector76] and the acyclic terpene utilization gene cluster in this organism has been identified and characterized [ForsterFromme06].

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 14-Dec-2010 by Fulcher CA , SRI International

Enzymatic reaction of: cis-geranyl-CoA carboxylase

EC Number:

cis-geranyl-CoA + hydrogen carbonate + ATP <=> 3-(4-methylpent-3-en-1-yl)-pent-2-enedioyl-CoA + ADP + phosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for cis-geranyl-CoA: 3-methylcrotonyl-CoA [Aguilar08 ]

In Pathways: cis-genanyl-CoA degradation

This enzyme catalyzes the condensation of cis-geranyl-CoA and hydrogen carbonate to form 3-(4-methylpent-3-en-1-yl)-pent-2-enedioyl-CoA (isohexenyl-glutaconyl-CoA). Purified, recombinant enzyme showed sigmoidal kinetics for cis-geranyl-CoA as substrate, but Michaelis-Menten kinetics for 3-methylcrotonyl-CoA and ATP as substrates [Aguilar08].

Cofactors or Prosthetic Groups: biotin [Aguilar08]

T(opt): 37 °C [Aguilar08]

pH(opt): 8.5 [Aguilar08]

Subunit of cis-geranyl-CoA carboxylase: cis-geranyl-CoA carboxylase α-subunit

Synonyms: AtuF, geranyl-CoA carboxylase, alpha-subunit (biotin-containing)

Gene: atuF Accession Number: G-12379 (MetaCyc)

Sequence Length: 661 AAs

Molecular Weight: 71.748 kD (from nucleotide sequence)

Molecular Weight: 74.0 kD (experimental) [Aguilar08]

Unification Links: Protein Model Portal:Q9HZV6 , SMR:Q9HZV6 , String:208964.PA2891 , UniProt:Q9HZV6

Relationship Links: InterPro:IN-FAMILY:IPR000089 , InterPro:IN-FAMILY:IPR001882 , InterPro:IN-FAMILY:IPR005479 , InterPro:IN-FAMILY:IPR005481 , InterPro:IN-FAMILY:IPR005482 , InterPro:IN-FAMILY:IPR011053 , InterPro:IN-FAMILY:IPR011054 , InterPro:IN-FAMILY:IPR011761 , InterPro:IN-FAMILY:IPR011764 , InterPro:IN-FAMILY:IPR013815 , InterPro:IN-FAMILY:IPR013816 , InterPro:IN-FAMILY:IPR016185 , Pfam:IN-FAMILY:PF00289 , Pfam:IN-FAMILY:PF00364 , Pfam:IN-FAMILY:PF02785 , Pfam:IN-FAMILY:PF02786 , Prosite:IN-FAMILY:PS00188 , Prosite:IN-FAMILY:PS00866 , Prosite:IN-FAMILY:PS00867 , Prosite:IN-FAMILY:PS50968 , Prosite:IN-FAMILY:PS50975 , Prosite:IN-FAMILY:PS50979 , Smart:IN-FAMILY:SM00878

The subunit apparent molecular mass was determined by SDS-PAGE [Aguilar08].

Subunit of cis-geranyl-CoA carboxylase: cis-geranyl-CoA carboxylase β-subunit

Synonyms: AtuC

Gene: atuC Accession Number: G-12378 (MetaCyc)

Sequence Length: 538 AAs

Molecular Weight: 57.329 kD (from nucleotide sequence)

Molecular Weight: 63.0 kD (experimental) [Aguilar08]

Unification Links: Protein Model Portal:Q9HZV9 , SMR:Q9HZV9 , String:208964.PA2888 , UniProt:Q9HZV9

Relationship Links: InterPro:IN-FAMILY:IPR000022 , InterPro:IN-FAMILY:IPR011762 , InterPro:IN-FAMILY:IPR011763 , InterPro:IN-FAMILY:IPR029045 , Pfam:IN-FAMILY:PF01039 , Prosite:IN-FAMILY:PS50980 , Prosite:IN-FAMILY:PS50989

The subunit apparent molecular mass was determined by SDS-PAGE [Aguilar08].


Aguilar08: Aguilar JA, Diaz-Perez C, Diaz-Perez AL, Rodriguez-Zavala JS, Nikolau BJ, Campos-Garcia J (2008). "Substrate specificity of the 3-methylcrotonyl coenzyme A (CoA) and geranyl-CoA carboxylases from Pseudomonas aeruginosa." J Bacteriol 190(14);4888-93. PMID: 18469096

Demyttenaere04: Demyttenaere JC, Vanoverschelde J, De Kimpe N (2004). "Biotransformation of (R)-(+)- and (S)-(-)-citronellol by Aspergillus sp. and Penicillium sp., and the use of solid-phase microextraction for screening." J Chromatogr A 1027(1-2);137-46. PMID: 14971495

Fall77: Fall RR, Hector ML (1977). "Acyl-coenzyme A carboxylases. Homologous 3-methylcrotonyl-CoA and geranyl-CoA carboxylases from Pseudomonas citronellolis." Biochemistry 16(18);4000-5. PMID: 911753

Fall81: Fall RR (1981). "3-Methylcrotonyl-CoA and geranyl-CoA carboxylases from Pseudomonas citronellolis." Methods Enzymol 71 Pt C;791-9. PMID: 7278686

ForsterFromme06: Forster-Fromme K, Jendrossek D (2006). "Identification and characterization of the acyclic terpene utilization gene cluster of Pseudomonas citronellolis." FEMS Microbiol Lett 264(2);220-5. PMID: 17064376

ForsterFromme10: Forster-Fromme K, Jendrossek D (2010). "Catabolism of citronellol and related acyclic terpenoids in pseudomonads." Appl Microbiol Biotechnol 87(3);859-69. PMID: 20490788

Hector76: Hector ML, Fall RR (1976). "Multiple acyl-coenzyme A carboxylases in Pseudomonas citronellolis." Biochemistry 15(16);3465-72. PMID: 8091

Hoschle05: Hoschle B, Gnau V, Jendrossek D (2005). "Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa." Microbiology 151(Pt 11);3649-56. PMID: 16272386

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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