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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
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MetaCyc Enzyme: GDP-D-mannose 4,6-dehydratase

Gene: gmd Accession Number: G-9848 (MetaCyc)

Synonyms: gca

Species: Pseudomonas aeruginosa

Subunit composition of GDP-D-mannose 4,6-dehydratase = [Gmd]4
         GDP-D-mannose 4,6-dehydratase subunit = Gmd

Summary:
Recombinant GDP-D-mannose 4,6-dehydratase from Pseudomonas aeruginosa has been expressed in Escherichia coli and purified, and the crystal structure has been determined [Webb04]. It is a member of the short-chain dehydrogenase/reductase (SDR) family, NDP-sugar modifying subfamily. Its homotetrameric structure is similar to that of the plant Arabidopsis thaliana enzyme, and differs from most other members of this subfamily, which are homodimers. It has been suggested that this tetrameric, dimer of dimers form may be the active oligomeric state of the enzyme in both prokaryotes and eukaryotes [Webb04].

Gene Citations: [Maki02]

Map Position: [6,143,109 <- 6,144,080]

Molecular Weight of Polypeptide: 36.399 kD (from nucleotide sequence)

Unification Links: Entrez-gene:883089 , Protein Model Portal:Q51366 , SMR:Q51366 , String:208964.PA5453 , UniProt:Q51366

Relationship Links: InterPro:IN-FAMILY:IPR001509 , InterPro:IN-FAMILY:IPR006368 , InterPro:IN-FAMILY:IPR016040 , Panther:IN-FAMILY:PTHR10366:SF32 , PDB:Structure:1RPN , Pfam:IN-FAMILY:PF01370

Gene-Reaction Schematic: ?

Credits:
Created 08-Jan-2007 by Fulcher CA , SRI International


Enzymatic reaction of: GDP-D-mannose 4,6-dehydratase

Synonyms: GDP-mannose 4,6-dehydratase, Gmd

EC Number: 4.2.1.47

GDP-α-D-mannose <=> GDP-4-dehydro-α-D-rhamnose + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis , GDP-D-rhamnose biosynthesis

Summary:
GDP-D-Mannose 4,6-dehydratase from Pseudomonas aeruginosa is able to use NADP+ or NAD+ as cofactor in vitro, although NADP(H) remains tightly bound during purification [Webb04].

Cofactors or Prosthetic Groups: NAD(P)H [Webb04]


References

Maki02: Maki M, Jarvinen N, Rabina J, Roos C, Maaheimo H, Renkonen R, , (2002). "Functional expression of Pseudomonas aeruginosa GDP-4-keto-6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose." Eur J Biochem 269(2);593-601. PMID: 11856318

Webb04: Webb NA, Mulichak AM, Lam JS, Rocchetta HL, Garavito RM (2004). "Crystal structure of a tetrameric GDP-D-mannose 4,6-dehydratase from a bacterial GDP-D-rhamnose biosynthetic pathway." Protein Sci 13(2);529-39. PMID: 14739333


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, biocyc14.