Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: L-carnitine dehydrogenase

Species: Xanthomonas translucens

Subunit composition of L-carnitine dehydrogenase = [L-carnitine dehydrogenase subunit]2

Summary:
L-carnitine dehydrogenase has been purified from Xanthomonas translucnes [Mori88]. The enzyme was specific to the L-carnitine enantiomer, and did not recognize D-carnitine or any other carnitine analogs such as choline and glycine betaine. It requires NAD+, and does not accept NADP+. The optimal pH was 9.5 for the oxidation reaction, and 6.5 for the reduction reaction.

Molecular Weight of Polypeptide: 37 kD (experimental) [Mori88 ]

Molecular Weight of Multimer: 74 kD (experimental) [Mori88]

Gene-Reaction Schematic: ?


Enzymatic reaction of: L-carnitine dehydrogenase

EC Number: 1.1.1.108

L-carnitine + NAD+ <=> 3-dehydrocarnitine + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

In Pathways: γ-butyrobetaine degradation , L-carnitine degradation II

Cofactors or Prosthetic Groups: NAD+ [Mori88]

Inhibitors (Unknown Mechanism): Ag+ [Mori88] , Ni2+ [Mori88] , Hg2+ [Mori88] , p-chloromercuribenzoate [Mori88] , Li+ [Mori88] , Ca2+ [Mori88] , Mn2+ [Mori88] , Co2+ [Mori88] , Cu2+ [Mori88] , Zn2+ [Mori88]

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-carnitine
10000.0
[Mori88]
NADH
40.0
[Mori88]
3-dehydrocarnitine
1710.0
[Mori88]
NAD+
250.0
[Mori88]

pH(opt): 9.5 [Mori88]


References

Mori88: Mori, N., Kasugai, T., Kitamato, Y., Ichikawa, Y. (1988). "Purification and some properties of carnitine dehydrogenase from Xanthomonas translucens." Agric. Biol. Chem. 52, 249-250.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC13A.