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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
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MetaCyc Enzyme: indole-3-acetaldehyde oxidase / indolepyruvate decarboxylase

Species: Sulfolobus tokodaii 7

Subunit composition of indole-3-acetaldehyde oxidase / indolepyruvate decarboxylase = [indolepyruvate decarboxylase subunit a]2[indolepyruvate decarboxylase subunit b]2[indolepyruvate decarboxylase subunit c]2

Summary:
An enzyme that catalyzes both the decarboxylation of indole-3-pyruvate and the subsequent oxidation of indole-3-acetaldehyde into indole-3-acetate was purified from the thermoacidophilic archaeon Sulfolobus sp. strain 7.

The enzyme is a hexamer made up of three subunits in a stoichiometry of 2:2:2. Both molybdenum and iron were detected, and biotin was suggested to bind to the b-subunit.

The first step, the decarboxylation reaction, was highly specific for 2-oxoacids with an aromatic group, while in the second reaction, various aldehydes including glyceraldehyde, which is a glycolytic intermediate in the organism, were oxidized [Wakagi02].

Molecular Weight: 280 kD (experimental) [Wakagi02 ]

Gene-Reaction Schematic: ?

Credits:
Created 21-Apr-2005 by Caspi R , SRI International


Enzymatic reaction of: indole-3-acetaldehyde oxidase

EC Number: 1.2.3.7

indole acetaldehyde + oxygen + H2O <=> indole-3-acetate + hydrogen peroxide + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Alternative Products for indole-3-acetate: phenylacetaldehyde [Wakagi02 ] , benzaldehyde [Wakagi02 ] , D-glyceraldehyde [Wakagi02 ]

In Pathways: tryptophan degradation VII (via indole-3-pyruvate)

Cofactors or Prosthetic Groups: biotin [Wakagi02], FMN [Wakagi02], Mo2+ [Wakagi02]

Kinetic Parameters:

Substrate
Km (μM)
Citations
indole acetaldehyde
9.0
[Wakagi02]

T(opt): 70 °C [Wakagi02]

pH(opt): 8 [Wakagi02]


Enzymatic reaction of: indolepyruvate decarboxylase

EC Number: 4.1.1.74

indole-3-pyruvate + H+ <=> CO2 + indole acetaldehyde

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for indole-3-pyruvate: 2-oxo-3-phenylpropanoate [Wakagi02 ]

In Pathways: tryptophan degradation VII (via indole-3-pyruvate)

Cofactors or Prosthetic Groups: FMN [Wakagi02], biotin [Wakagi02], Mo2+ [Wakagi02]

Kinetic Parameters:

Substrate
Km (μM)
Citations
indole-3-pyruvate
788.0
[Wakagi02]
indole acetaldehyde
9.0
[Wakagi02]

T(opt): 70 °C [Wakagi02]

pH(opt): 8 [Wakagi02]


Subunit of indole-3-acetaldehyde oxidase / indolepyruvate decarboxylase: indolepyruvate decarboxylase subunit a

Molecular Weight: 89 kD (experimental) [Wakagi02]


Subunit of indole-3-acetaldehyde oxidase / indolepyruvate decarboxylase: indolepyruvate decarboxylase subunit b

Molecular Weight: 30 kD (experimental) [Wakagi02]


Subunit of indole-3-acetaldehyde oxidase / indolepyruvate decarboxylase: indolepyruvate decarboxylase subunit c

Molecular Weight: 19 kD (experimental) [Wakagi02]


References

Wakagi02: Wakagi T, Fukuda E, Ogawa Y, Kino H, Matsuzawa H (2002). "A novel bifunctional molybdo-enzyme catalyzing both decarboxylation of indolepyruvate and oxidation of indoleacetaldehyde from a thermoacidophilic archaeon, Sulfolobus sp. strain 7." FEBS Lett 510(3);196-200. PMID: 11801253


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14A.