|Gene:||ipdC||Accession Number: G-5861 (MetaCyc)|
Species: Enterobacter cloacae
Subunit composition of
indolepyruvate decarboxylase = [IpdC]4
indole-3-pyruvate decarboxylase subunit = IpdC
Idole-3-pyruvate decarboxylase is the key enzyme in the pathway leading from tryptophan to indole acetate (IAA), since the other two enzymes, L-tryptophan aminotransferase and indole-3-acetaldehyde oxidase, are present in most bacteria, including those incapable of producing IAA [Koga91, Koga92].
This enzyme is a homotetramer, which in the absence of thiamine pyrophosphate (TPP) and Mg2+ dissociates into inactive monomers and dimers. It has high specificity and affinity only for its substrate, indole-3-pyruvate [Koga92].
Molecular Weight of Polypeptide: 60.024 kD (from nucleotide sequence), 60 kD (experimental) [Koga92 ]
Molecular Weight of Multimer: 240 kD (experimental) [Koga92]
Relationship Links: Entrez-Nucleotide:PART-OF:X80715 , Entrez-Nucleotide:PART-OF:X80716 , InterPro:IN-FAMILY:IPR000399 , InterPro:IN-FAMILY:IPR011766 , InterPro:IN-FAMILY:IPR012000 , InterPro:IN-FAMILY:IPR012001 , InterPro:IN-FAMILY:IPR012110 , InterPro:IN-FAMILY:IPR017764 , InterPro:IN-FAMILY:IPR029035 , InterPro:IN-FAMILY:IPR029061 , Panther:IN-FAMILY:PTHR18968:SF4 , PDB:Structure:1OVM , Pfam:IN-FAMILY:PF00205 , Pfam:IN-FAMILY:PF02775 , Pfam:IN-FAMILY:PF02776 , Prosite:IN-FAMILY:PS00187
|MultiFun Terms:||metabolism → carbon utilization → amino acids|
Enzymatic reaction of: indolepyruvate decarboxylase
Synonyms: indole-3-pyruvare decarboxylase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: L-tryptophan degradation VII (via indole-3-pyruvate)
pH(opt): 6.5 [Koga92]
Koga92: Koga J, Adachi T, Hidaka H (1992). "Purification and characterization of indolepyruvate decarboxylase. A novel enzyme for indole-3-acetic acid biosynthesis in Enterobacter cloacae." J Biol Chem 267(22);15823-8. PMID: 1639814
Schutz03: Schutz A, Sandalova T, Ricagno S, Hubner G, Konig S, Schneider G (2003). "Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid." Eur J Biochem 270(10);2312-21. PMID: 12752451
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