MetaCyc Enzyme: indolepyruvate decarboxylase

Gene: ipdC Accession Number: G-5861 (MetaCyc)

Species: Enterobacter cloacae

Subunit composition of indolepyruvate decarboxylase = [IpdC]4
         indole-3-pyruvate decarboxylase subunit = IpdC

Idole-3-pyruvate decarboxylase is the key enzyme in the pathway leading from tryptophan to indole acetate (IAA), since the other two enzymes, L-tryptophan aminotransferase and indole-3-acetaldehyde oxidase, are present in most bacteria, including those incapable of producing IAA [Koga91, Koga92].

This enzyme is a homotetramer, which in the absence of thiamine pyrophosphate (TPP) and Mg2+ dissociates into inactive monomers and dimers. It has high specificity and affinity only for its substrate, indole-3-pyruvate [Koga92].

Molecular Weight of Polypeptide: 60.024 kD (from nucleotide sequence), 60 kD (experimental) [Koga92 ]

Molecular Weight of Multimer: 240 kD (experimental) [Koga92]

Unification Links: ModBase:P23234 , Protein Model Portal:P23234 , SMR:P23234 , Swiss-Model:P23234 , UniProt:P23234

Relationship Links: Entrez-Nucleotide:PART-OF:X80715 , Entrez-Nucleotide:PART-OF:X80716 , InterPro:IN-FAMILY:IPR000399 , InterPro:IN-FAMILY:IPR011766 , InterPro:IN-FAMILY:IPR012000 , InterPro:IN-FAMILY:IPR012001 , InterPro:IN-FAMILY:IPR012110 , InterPro:IN-FAMILY:IPR017764 , InterPro:IN-FAMILY:IPR029035 , InterPro:IN-FAMILY:IPR029061 , Panther:IN-FAMILY:PTHR18968:SF4 , PDB:Structure:1OVM , Pfam:IN-FAMILY:PF00205 , Pfam:IN-FAMILY:PF02775 , Pfam:IN-FAMILY:PF02776 , Prosite:IN-FAMILY:PS00187

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

MultiFun Terms: metabolism carbon utilization amino acids

Created 18-Apr-2005 by Caspi R , SRI International

Enzymatic reaction of: indolepyruvate decarboxylase

Synonyms: indole-3-pyruvare decarboxylase

EC Number:

indole-3-pyruvate + H+ <=> CO2 + indole acetaldehyde

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: L-tryptophan degradation VII (via indole-3-pyruvate)

Cofactors or Prosthetic Groups: Mg2+ [Koga92], thiamin diphosphate [Koga92]

Inhibitors (Competitive): pyruvate [Koga92] , 2-oxoglutarate [Koga92] , 2-oxo-3-phenylpropanoate [Koga92]

Kinetic Parameters:

Km (μM)

pH(opt): 6.5 [Koga92]


Koga91: Koga J, Adachi T, Hidaka H (1991). "Molecular cloning of the gene for indolepyruvate decarboxylase from Enterobacter cloacae." Mol Gen Genet 226(1-2);10-6. PMID: 2034209

Koga92: Koga J, Adachi T, Hidaka H (1992). "Purification and characterization of indolepyruvate decarboxylase. A novel enzyme for indole-3-acetic acid biosynthesis in Enterobacter cloacae." J Biol Chem 267(22);15823-8. PMID: 1639814

Schutz03: Schutz A, Sandalova T, Ricagno S, Hubner G, Konig S, Schneider G (2003). "Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid." Eur J Biochem 270(10);2312-21. PMID: 12752451

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu Oct 8, 2015, biocyc13.