Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: acetate kinase

Gene: ack Accession Number: G-383 (MetaCyc)

Species: Methanosarcina thermophila

Subunit composition of acetate kinase = [Ack]2

Molecular Weight of Polypeptide: 44.337 kD (from nucleotide sequence) [Bairoch, 1993 ]

Gene-Reaction Schematic: ?


Enzymatic reaction of: acetate kinase

Synonyms: acetokinase, ATP:acetate phosphotransferase

EC Number: 2.7.2.1

ATP + acetate <=> ADP + acetyl phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for acetate [Aceti88 ]: propanoate

Alternative Substrates for ATP [Aceti88 ]: dTTP , ITP , UTP , GTP , CTP

In Pathways: superpathway of methanogenesis , acetate formation from acetyl-CoA I , methanogenesis from acetate

Summary:
Acetate kinase catalyzes the first reaction in the activation of acetate during methanogenesis. The enzyme from Methanosarcina thermophila is stable to 70oC. [Ferry92, Thauer98, Aceti88, SinghWissmann95, Latimer93, Ferry93]

Cofactors or Prosthetic Groups: Mg2+

Alternative Cofactors for Mg2+: Mn2+ , Co2+ , Ca2+

Inhibitors (Unknown Mechanism): diethylpyrocarbonate [Latimer93] , phenylglyoxal [Latimer93]


References

Aceti88: Aceti DJ, Ferry JG (1988). "Purification and characterization of acetate kinase from acetate-grown Methanosarcina thermophila. Evidence for regulation of synthesis." J Biol Chem 1988;263(30);15444-8. PMID: 2844814

Bairoch, 1993: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529

Ferry92: Ferry JG (1992). "Methane from acetate." J Bacteriol 1992;174(17);5489-95. PMID: 1512186

Ferry93: Ferry, J. G. editor (1993). "Methanogenesis: Ecology, Physiology, Biochemistry & Genetics. Chapman & Hall, New York."

Latimer93: Latimer MT, Ferry JG (1993). "Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila." J Bacteriol 1993;175(21);6822-9. PMID: 8226623

SinghWissmann95: Singh-Wissmann K, Ferry JG (1995). "Transcriptional regulation of the phosphotransacetylase-encoding and acetate kinase-encoding genes (pta and ack) from Methanosarcina thermophila." J Bacteriol 1995;177(7);1699-702. PMID: 7896690

Thauer98: Thauer RK (1998). "Biochemistry of methanogenesis: a tribute to Marjory Stephenson." Microbiology 1998;144 ( Pt 9);2377-406. PMID: 9782487


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, biocyc13.