MetaCyc Enzyme: acetate kinase

Gene: ack Accession Number: G-383 (MetaCyc)

Species: Methanosarcina thermophila

Subunit composition of acetate kinase = [Ack]2

Molecular Weight of Polypeptide: 44.337 kD (from nucleotide sequence) [Bairoch93a ]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Enzymatic reaction of: acetate kinase

Synonyms: acetokinase, ATP:acetate phosphotransferase

EC Number:

ATP + acetate <=> ADP + acetyl phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for acetate [Aceti88 ]: propanoate

Alternative Substrates for ATP [Aceti88 ]: dTTP , ITP , UTP , GTP , CTP

In Pathways: superpathway of methanogenesis , acetate formation from acetyl-CoA I , methanogenesis from acetate

Acetate kinase catalyzes the first reaction in the activation of acetate during methanogenesis. The enzyme from Methanosarcina thermophila is stable to 70oC. [Ferry92, Thauer98, Aceti88, SinghWissmann95, Latimer93, Ferry93]

Cofactors or Prosthetic Groups: Mg2+

Alternative Cofactors for Mg2+: Mn2+ , Co2+ , Ca2+

Inhibitors (Unknown Mechanism): diethylpyrocarbonate [Latimer93] , phenylglyoxal [Latimer93]


Aceti88: Aceti DJ, Ferry JG (1988). "Purification and characterization of acetate kinase from acetate-grown Methanosarcina thermophila. Evidence for regulation of synthesis." J Biol Chem 1988;263(30);15444-8. PMID: 2844814

Bairoch93a: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529

Ferry92: Ferry JG (1992). "Methane from acetate." J Bacteriol 1992;174(17);5489-95. PMID: 1512186

Ferry93: Ferry, J. G. editor (1993). "Methanogenesis: Ecology, Physiology, Biochemistry & Genetics. Chapman & Hall, New York."

Latimer93: Latimer MT, Ferry JG (1993). "Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila." J Bacteriol 1993;175(21);6822-9. PMID: 8226623

SinghWissmann95: Singh-Wissmann K, Ferry JG (1995). "Transcriptional regulation of the phosphotransacetylase-encoding and acetate kinase-encoding genes (pta and ack) from Methanosarcina thermophila." J Bacteriol 1995;177(7);1699-702. PMID: 7896690

Thauer98: Thauer RK (1998). "Biochemistry of methanogenesis: a tribute to Marjory Stephenson." Microbiology 1998;144 ( Pt 9);2377-406. PMID: 9782487

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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