|Gene:||ack||Accession Number: G-383 (MetaCyc)|
Species: Methanosarcina thermophila
Subunit composition of acetate kinase = [Ack]2
Molecular Weight of Polypeptide: 44.337 kD (from nucleotide sequence) [Bairoch93a ]
Enzymatic reaction of: acetate kinase
Synonyms: acetokinase, ATP:acetate phosphotransferase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
This reaction is reversible.
Acetate kinase catalyzes the first reaction in the activation of acetate during methanogenesis. The enzyme from Methanosarcina thermophila is stable to 70oC. [Ferry92, Thauer98, Aceti88, SinghWissmann95, Latimer93, Ferry93]
Cofactors or Prosthetic Groups: Mg2+
Aceti88: Aceti DJ, Ferry JG (1988). "Purification and characterization of acetate kinase from acetate-grown Methanosarcina thermophila. Evidence for regulation of synthesis." J Biol Chem 1988;263(30);15444-8. PMID: 2844814
Latimer93: Latimer MT, Ferry JG (1993). "Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila." J Bacteriol 1993;175(21);6822-9. PMID: 8226623
SinghWissmann95: Singh-Wissmann K, Ferry JG (1995). "Transcriptional regulation of the phosphotransacetylase-encoding and acetate kinase-encoding genes (pta and ack) from Methanosarcina thermophila." J Bacteriol 1995;177(7);1699-702. PMID: 7896690
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