|Gene:||pcaB||Accession Number: G-2287 (MetaCyc)|
Synonyms: β-carboxy-cis,cis-muconate lactonizing enzyme, 3-carboxy-cis,cis-muconate lactonizing enzyme, CMLE
Species: Pseudomonas putida
Subunit composition of
3-carboxymuconate cycloisomerase = [PcaB]4
subunit of 3-carboxymuconate cycloisomerase = PcaB
The deduced amino acid sequence of 3-carboxymuconate cycloisomerase (CMLE) has significant sequence homology to the class II fumarase family of proteins [Williams92]. Contrarily, CMLE has no significant similarity to cis, cis-muconate lactonizing enzyme (MLE), the enzyme which catalyzes the analogous reaction in the catechol branch of the β-ketodadipate pathway. The reaction mechanisms of CMLE and MLE were also found to be different; while CMLE catalyzes an anti-cycloisomerization reaction, MLE catalyzes a syn- syn-cycloisomerization reaction (as reported in [Williams92]). These findings suggest that CMLE and MLE have a remote evolutionary relationship despite that they catalyze similar reactions [Williams92].
Molecular Weight of Multimer: 190 kD (experimental) [Patel73]
Relationship Links: InterPro:IN-FAMILY:IPR000362 , InterPro:IN-FAMILY:IPR008948 , InterPro:IN-FAMILY:IPR012789 , InterPro:IN-FAMILY:IPR019468 , InterPro:IN-FAMILY:IPR020557 , InterPro:IN-FAMILY:IPR022761 , InterPro:IN-FAMILY:IPR024083 , Panther:IN-FAMILY:PTHR11444 , Pfam:IN-FAMILY:PF00206 , Pfam:IN-FAMILY:PF10397 , Prints:IN-FAMILY:PR00149 , Prosite:IN-FAMILY:PS00163 , Smart:IN-FAMILY:SM00998
Enzymatic reaction of: 3-carboxymuconate cycloisomerase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the opposite direction.
Ornston66b: Ornston LN (1966). "The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. II. Enzymes of the protocatechuate pathway." J Biol Chem 241(16);3787-94. PMID: 5916392
Patel73: Patel RN, Meagher RB, Ornston LN (1973). "Relationships among enzymes of the beta-ketoadipate pathway. II. Properties of crystalline beta-carboxy-cis,cis-muconate-lactonizing enzyme from Pseudomonas putida." Biochemistry 12(18);3531-7. PMID: 4199895
Williams92: Williams SE, Woolridge EM, Ransom SC, Landro JA, Babbitt PC, Kozarich JW (1992). "3-Carboxy-cis,cis-muconate lactonizing enzyme from Pseudomonas putida is homologous to the class II fumarase family: a new reaction in the evolution of a mechanistic motif." Biochemistry 31(40);9768-76. PMID: 1390752
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