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discounted EARLY registration ends Dec 31, 2014
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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: phosphatidylinositol/ inositol polyphosphate 5-phosphatase

Gene: 5PTase14 Accession Number: AT2G31830 (MetaCyc)

Synonyms: inositol polyphosphate 5-phosphatase (type II)

Species: Arabidopsis thaliana col

Summary:
5PTase14 has Mg2+-dependent inositol polyphosphate 5-phosphatase activity against a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate with lower levels of activity against 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate and the soluble D-myo-inositol (1,4,5)-trisphosphate when its activity is tested in vitro following expression in yeast. In the same assays, it shows no activity against several other membrane-based phosphoinositides (a 1-phosphatidyl-1D-myo-inositol 3-phosphate, a 1-phosphatidyl-1D-myo-inositol 4-phosphate, a 1-phosphatidyl-1D-myo-inositol 5-phosphate, a 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate, or 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate), nor does it show any activity with the soluble tetraphosphate inositol D-myo-inositol (1,3,4,5)-tetrakisphosphate [Zhong04].

Interestingly, in addition to its type II 5PTase domain, this enzyme also has a WD40 domain which is typically associated with protein-protein interactions.

5PTase14 transcripts appear to be expressed at higher levels in seedlings and inflorescence apices than in other organs. In addition, 5PTase14 transcript expression levels appear to decrease slightly in response to a dark treatment [Zhong04].

Map Position: [13,539,853 -> 13,544,818]

Unification Links: Phytozome Plant Orthologs:AT2G31830.1 , TAIR:AT2G31830 , UniProt:Q9SKB7

Gene-Reaction Schematic: ?

Gene Class: UNCLASSIFIED

Credits:
Imported from AraCyc 12-Apr-2012 by Dreher KA , PMN


Enzymatic reaction of: PI(4,5)P2phosphoinositide 5-phosphatase (phosphatidylinositol/ inositol polyphosphate 5-phosphatase)

EC Number: 3.1.3.36

a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O <=> a 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Credits:
Imported from AraCyc 12-Apr-2012 by Dreher KA , PMN

Summary:
The Vmax with a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is 468 pmol/µg/min [Zhong04].

Activators (Unknown Mechanism): Mg2+ [Zhong04]

Kinetic Parameters:

Substrate
Km (μM)
a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
101.0


Enzymatic reaction of: Ins(1,4,5)P3 5-phosphatase (phosphatidylinositol/ inositol polyphosphate 5-phosphatase)

D-myo-inositol (1,4,5)-trisphosphate + H2O <=> D-myo-inositol (1,4)-bisphosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: D-myo-inositol (1,4,5)-trisphosphate degradation

Summary:
The Vmax with D-myo-inositol (1,4,5)-trisphosphate is 429 pmol/µg/min [Zhong04].

Activators (Unknown Mechanism): Mg2+ [Zhong04]

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-myo-inositol (1,4,5)-trisphosphate
395.0
[Zhong04]


Enzymatic reaction of: PI(3,4,5)P3 5-phosphatase (phosphatidylinositol/ inositol polyphosphate 5-phosphatase)

EC Number: 3.1.3.86

1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O <=> a 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Summary:
The Vmax with 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate is 235 pmol/µg/min [Zhong04].

As of 2010, PI(3,4,5)P3 had not been identified in plants so it is unclear whether this in vitro activity has any biological relevance [Zhao10c].

Activators (Unknown Mechanism): Mg2+ [Zhong04]

Kinetic Parameters:

Substrate
Km (μM)
Citations
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
211.0
[Zhong04]


References

Carricaburu03: Carricaburu V, Lamia KA, Lo E, Favereaux L, Payrastre B, Cantley LC, Rameh LE (2003). "The phosphatidylinositol (PI)-5-phosphate 4-kinase type II enzyme controls insulin signaling by regulating PI-3,4,5-trisphosphate degradation." Proc Natl Acad Sci U S A 100(17);9867-72. PMID: 12897244

Chi09: Chi A, Rhee S (2009). "The functional annotation of Arabidopsis protein sequences was performed by BLAST queries against a reference set of experimentally verified enzymes. For each Arabidopsis sequence, the enzymatic activity of the top BLAST hit (or hits if they had equivalent E-values) was assigned to the protein if its E-value fell below a specific E-value threshold established for the corresponding enzymatic activity. Note: The annotation thresholds were established by doing a self BLAST of the reference enzyme dataset. For each enzymatic activity represented by multiple proteins, the mean E-value of all the correct hits generated by the self BLAST was selected as the cut-off. All of these means were averaged and used as the cut-off for assigning annotations for any enzymatic activities that were represented by a single protein in the reference dataset."

Clarke01a: Clarke JH, Letcher AJ, D'santos CS, Halstead JR, Irvine RF, Divecha N (2001). "Inositol lipids are regulated during cell cycle progression in the nuclei of murine erythroleukaemia cells." Biochem J 357(Pt 3);905-10. PMID: 11463365

COMMUNICATIONa: Communication, http://arabidopsis.org/servlets/TairObject?accession=Communication:501714663.

Gozani03: Gozani O, Karuman P, Jones DR, Ivanov D, Cha J, Lugovskoy AA, Baird CL, Zhu H, Field SJ, Lessnick SL, Villasenor J, Mehrotra B, Chen J, Rao VR, Brugge JS, Ferguson CG, Payrastre B, Myszka DG, Cantley LC, Wagner G, Divecha N, Prestwich GD, Yuan J (2003). "The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor." Cell 114(1);99-111. PMID: 12859901

Ivetac05: Ivetac I, Munday AD, Kisseleva MV, Zhang XM, Luff S, Tiganis T, Whisstock JC, Rowe T, Majerus PW, Mitchell CA (2005). "The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane." Mol Biol Cell 16(5);2218-33. PMID: 15716355

Lecompte08: Lecompte O, Poch O, Laporte J (2008). "PtdIns5P regulation through evolution: roles in membrane trafficking?." Trends Biochem Sci 33(10);453-60. PMID: 18774718

Meijer01: Meijer HJ, Berrie CP, Iurisci C, Divecha N, Musgrave A, Munnik T (2001). "Identification of a new polyphosphoinositide in plants, phosphatidylinositol 5-monophosphate (PtdIns5P), and its accumulation upon osmotic stress." Biochem J 360(Pt 2);491-8. PMID: 11716778

Morris00: Morris JB, Hinchliffe KA, Ciruela A, Letcher AJ, Irvine RF (2000). "Thrombin stimulation of platelets causes an increase in phosphatidylinositol 5-phosphate revealed by mass assay." FEBS Lett 475(1);57-60. PMID: 10854858

Pagliarini04: Pagliarini DJ, Worby CA, Dixon JE (2004). "A PTEN-like phosphatase with a novel substrate specificity." J Biol Chem 279(37);38590-6. PMID: 15247229

Rohde02: Rohde G, Wenzel D, Haucke V (2002). "A phosphatidylinositol (4,5)-bisphosphate binding site within mu2-adaptin regulates clathrin-mediated endocytosis." J Cell Biol 158(2);209-14. PMID: 12119359

Schaletzky03: Schaletzky J, Dove SK, Short B, Lorenzo O, Clague MJ, Barr FA (2003). "Phosphatidylinositol-5-phosphate activation and conserved substrate specificity of the myotubularin phosphatidylinositol 3-phosphatases." Curr Biol 13(6);504-9. PMID: 12646134

Shearn01: Shearn CT, Walker J, Norris FA (2001). "Identification of a novel spliceoform of inositol polyphosphate 4-phosphatase type Ialpha expressed in human platelets: structure of human inositol polyphosphate 4-phosphatase type I gene." Biochem Biophys Res Commun 286(1);119-25. PMID: 11485317

Zhang07d: Zhang Y, Zolov SN, Chow CY, Slutsky SG, Richardson SC, Piper RC, Yang B, Nau JJ, Westrick RJ, Morrison SJ, Meisler MH, Weisman LS (2007). "Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice." Proc Natl Acad Sci U S A 104(44);17518-23. PMID: 17956977

Zhang98h: Zhang X, Majerus PW (1998). "Phosphatidylinositol signalling reactions." Semin Cell Dev Biol 9(2);153-60. PMID: 9599410

Zhao10c: Zhao Y, Yan A, Feijo JA, Furutani M, Takenawa T, Hwang I, Fu Y, Yang Z (2010). "Phosphoinositides regulate clathrin-dependent endocytosis at the tip of pollen tubes in Arabidopsis and tobacco." Plant Cell 22(12);4031-44. PMID: 21189293

Zhong04: Zhong R, Ye ZH (2004). "Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana." Plant Cell Physiol 45(11);1720-8. PMID: 15574849


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.