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MetaCyc Enzyme: NADH oxidoreductase

Gene: hcr Accession Numbers: G6456 (MetaCyc), b0872, ECK0863

Synonyms: ybjV

Species: Escherichia coli K-12 substr. MG1655

Summary:
The hcr gene encodes an NADH oxidoreductase that catalyzes the reduction of the hybrid-cluster protein (HCP). The physiological roles of HCP and the oxidoreductase are not known. They are detected only during anaerobic growth in the presence of nitrate and nitrite [vandenBerg00].

Map Position: [910,405 <- 911,373]

Molecular Weight of Polypeptide: 35.74 kD (from nucleotide sequence), 34.6 kD (experimental) [vandenBerg00 ]

Unification Links: ASAP:ABE-0002960 , EchoBASE:EB3455 , EcoGene:EG13691 , EcoliWiki:b0872 , ModBase:P75824 , OU-Microarray:b0872 , PortEco:hcr , PR:PRO_000022866 , Protein Model Portal:P75824 , RefSeq:NP_415393 , RegulonDB:G6456 , SMR:P75824 , String:511145.b0872 , UniProt:P75824

Relationship Links: InterPro:IN-FAMILY:IPR000951 , InterPro:IN-FAMILY:IPR001041 , InterPro:IN-FAMILY:IPR001433 , InterPro:IN-FAMILY:IPR008333 , InterPro:IN-FAMILY:IPR012675 , InterPro:IN-FAMILY:IPR017927 , InterPro:IN-FAMILY:IPR017938 , Pfam:IN-FAMILY:PF00111 , Pfam:IN-FAMILY:PF00175 , Pfam:IN-FAMILY:PF00970 , Prints:IN-FAMILY:PR00409 , Prosite:IN-FAMILY:PS00197 , Prosite:IN-FAMILY:PS51085 , Prosite:IN-FAMILY:PS51384

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0016651 - oxidoreductase activity, acting on NAD(P)H Inferred from experiment [vandenBerg00]
GO:0050660 - flavin adenine dinucleotide binding Inferred from experiment [vandenBerg00]
GO:0051537 - 2 iron, 2 sulfur cluster binding Inferred from experiment Inferred by computational analysis [UniProtGOA11a, vandenBerg00]
GO:0009055 - electron carrier activity Inferred by computational analysis [GOA01a]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
GO:0051536 - iron-sulfur cluster binding Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: metabolism

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: NADH oxidoreductase

Synonyms: HCP oxidoreductase, hybrid-cluster protein oxidoreductase

EC Number: 1.6.-.-

hybrid-cluster proteinox + NADH <=> hybrid-cluster proteinred + NAD+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Alternative Substrates for hybrid-cluster proteinox: oxidized DCPIP [vandenBerg00 ]

Alternative Substrates for NADH: NADPH [vandenBerg00 ]

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Cofactors or Prosthetic Groups: [2Fe-2S] iron-sulfur cluster [vandenBerg00], FAD [vandenBerg00]

Kinetic Parameters:

Substrate
Km (μM)
Citations
NADH
10.0
[vandenBerg00]
NADPH
300.0
[vandenBerg00]


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 7 -> 107
[UniProt09]
UniProt: FAD-binding FR-type;
Protein-Segment 111 -> 213
[UniProt10a]
UniProt: Oxidoreductase; Sequence Annotation Type: region of interest;
Conserved-Region 237 -> 322
[UniProt09]
UniProt: 2Fe-2S ferredoxin-type;
Metal-Binding-Site 273
[UniProt10a]
UniProt: Iron-sulfur (2Fe-2S); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 278
[UniProt10a]
UniProt: Iron-sulfur (2Fe-2S); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 281
[UniProt10a]
UniProt: Iron-sulfur (2Fe-2S); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 311
[UniProt10a]
UniProt: Iron-sulfur (2Fe-2S); Non-Experimental Qualifier: by similarity;

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

vandenBerg00: van den Berg WA, Hagen WR, van Dongen WM (2000). "The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2Fe-2S]." Eur J Biochem 2000;267(3);666-76. PMID: 10651802


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, BIOCYC13A.