|Gene:||glxK||Accession Numbers: G6283 (MetaCyc), b0514, ECK0507|
Synonyms: glxB5, ybbZ
Species: Escherichia coli K-12 substr. MG1655
Glycerate kinase II (GKII), encoded by glxK, catalyzes the formation of 3-phosphoglycerate from D-glycerate [Doughty66].
There are two glycerate kinases, known as GKI and GKII, in E. coli. GKII is less thermostable and has a narrower pH optimum than GKI [Ornston69].
A glxK mutant is unable to utilize glyoxylate [Cusa99].
|Map Position: [541,112 -> 542,257]|
Molecular Weight of Polypeptide: 38.734 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0001773 , EchoBASE:EB3386 , EcoGene:EG13621 , EcoliWiki:b0514 , ModBase:P77364 , OU-Microarray:b0514 , PortEco:glxK , PR:PRO_000022815 , Pride:P77364 , Protein Model Portal:P77364 , RefSeq:NP_415047 , RegulonDB:G6283 , SMR:P77364 , String:511145.b0514 , UniProt:P77364
|Biological Process:||GO:0009436 - glyoxylate catabolic process
GO:0046296 - glycolate catabolic process [UniProtGOA12, Ornston69]
GO:0016310 - phosphorylation [UniProtGOA11a]
GO:0031388 - organic acid phosphorylation [GOA01a]
|Molecular Function:||GO:0008887 - glycerate kinase activity
[GOA01, GOA01a, Doughty66]
GO:0000166 - nucleotide binding [UniProtGOA11a]
GO:0005524 - ATP binding [UniProtGOA11a]
GO:0016301 - kinase activity [UniProtGOA11a]
GO:0016740 - transferase activity [UniProtGOA11a]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09]|
|MultiFun Terms:||metabolism → carbon utilization → carbon compounds|
|metabolism → central intermediary metabolism → glycolate metabolism|
|metabolism → central intermediary metabolism → glyoxylate degradation|
Enzymatic reaction of: glycerate kinase
Synonyms: D-glycerate 3-kinase, D-glycerate kinase, ATP:glycerate 3-phosphotransferase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
The reaction is physiologically favored in the direction shown.
Alternative Substrates [Comment 1]:
Purification of the enzyme and identification of 3-phosphoglycerate as the product of the reaction was done with E. coli Crook's strain [Doughty66].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Cusa99: Cusa E, Obradors N, Baldoma L, Badia J, Aguilar J (1999). "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli." J Bacteriol 1999;181(24);7479-84. PMID: 10601204
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
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