|Gene:||Bx3||Accession Number: G-7862 (MetaCyc)|
Species: Zea mays
The maize Bx3 gene encodes a cytochrome P-450-dependent monooxygenase [Glawischnig97] with indolin-2-one monooxygenase activity (BX3). The activity of this protein was tested using microsomal fractions isolated from transgenic yeast strains expressing maize Bx3 [Frey97]. This protein was also shown to catalyze the conversion of benzoxazin-3-one into HBOA [Glawischnig99]. To date this protein has not been purified to homogeneity.
Molecular Weight of Polypeptide: 59.7 kD (from nucleotide sequence), 59.7 kD (experimental) [Frey95 ]
Relationship Links: Entrez-Nucleotide:PART-OF:Y11404 , InterPro:IN-FAMILY:IPR001128 , InterPro:IN-FAMILY:IPR002401 , InterPro:IN-FAMILY:IPR017972 , Pfam:IN-FAMILY:PF00067 , Prints:IN-FAMILY:PR00385 , Prints:IN-FAMILY:PR00463 , Prosite:IN-FAMILY:PS00086
Enzymatic reaction of: indolin-2-one monooxygenase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
Frey97: Frey M, Chomet P, Glawischnig E, Stettner C, Grun S, Winklmair A, Eisenreich W, Bacher A, Meeley RB, Briggs SP, Simcox K, Gierl A (1997). "Analysis of a chemical plant defense mechanism in grasses." Science 277(5326);696-9. PMID: 9235894
Glawischnig99: Glawischnig E, Grun S, Frey M, Gierl A (1999). "Cytochrome P450 monooxygenases of DIBOA biosynthesis: specificity and conservation among grasses." Phytochemistry 50(6);925-30. PMID: 10385992
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