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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: indolin-2-one monooxygenase

Gene: Bx3 Accession Number: G-7862 (MetaCyc)

Synonyms: cyp71c2

Species: Zea mays

Summary:
The maize Bx3 gene encodes a cytochrome P-450-dependent monooxygenase [Glawischnig97] with indolin-2-one monooxygenase activity (BX3). The activity of this protein was tested using microsomal fractions isolated from transgenic yeast strains expressing maize Bx3 [Frey97]. This protein was also shown to catalyze the conversion of benzoxazin-3-one into HBOA [Glawischnig99]. To date this protein has not been purified to homogeneity.

Molecular Weight of Polypeptide: 59.7 kD (from nucleotide sequence), 59.7 kD (experimental) [Frey95 ]

Unification Links: Entrez:CAA72208 , Pride:Q43255 , Protein Model Portal:Q43255 , UniProt:Q43255

Relationship Links: Entrez-Nucleotide:PART-OF:Y11404 , InterPro:IN-FAMILY:IPR001128 , InterPro:IN-FAMILY:IPR002401 , InterPro:IN-FAMILY:IPR017972 , Pfam:IN-FAMILY:PF00067 , Prints:IN-FAMILY:PR00385 , Prints:IN-FAMILY:PR00463 , Prosite:IN-FAMILY:PS00086

Gene-Reaction Schematic: ?

Credits:
Revised 04-Apr-2011 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: indolin-2-one monooxygenase

EC Number: 1.14.13.138

indolin-2-one + NAD(P)H + oxygen + H+ <=> 3-hydroxyindolin-2-one + NAD(P)+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of benzoxazinoid glucosides biosynthesis , DIBOA-glucoside biosynthesis


References

Frey95: Frey M, Kliem R, Saedler H, Gierl A (1995). "Expression of a cytochrome P450 gene family in maize." Mol Gen Genet 246(1);100-9. PMID: 7823905

Frey97: Frey M, Chomet P, Glawischnig E, Stettner C, Grun S, Winklmair A, Eisenreich W, Bacher A, Meeley RB, Briggs SP, Simcox K, Gierl A (1997). "Analysis of a chemical plant defense mechanism in grasses." Science 277(5326);696-9. PMID: 9235894

Glawischnig97: Glawischnig, E., Eisenreich W., Bacher A., Frey M., Gierl A. (1997). "Biosynthetic origin of oxygen atoms in DIMBOA from maize: NMR studies of 18O2." Phytochemistry 45(4):715-718.

Glawischnig99: Glawischnig E, Grun S, Frey M, Gierl A (1999). "Cytochrome P450 monooxygenases of DIBOA biosynthesis: specificity and conservation among grasses." Phytochemistry 50(6);925-30. PMID: 10385992


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, biocyc14.