MetaCyc Polypeptide: GcdC

Gene: gcdC Accession Number: G-636 (MetaCyc)

Synonyms: glutaconyl-CoA decarboxylase subunit C, glutaconyl-CoA decarboxylase γ subunit, biotin carrier subunit of glutaconyl-CoA decarboxylase

Species: Acidaminococcus fermentans

Component of: glutaconyl-CoA decarboxylase (summary available)

GcdC is the biotin carrier. This subunit contains a flexible stretch of 50 amino acid residues, which consists of 34 alanines, 14 prolines, one valine, and one lysine. The large discrepancy between the molecular weight calculated from the deduced amino acid sequence and that obtained experimentally via SDS-PAGE is probably due to the extended alanine/proline region.

GcdC shares high amino acid sequence identities with the biotin carriers of methylmalonyl-CoA decarboxylases (MmdC) from Veillonella parvula and Propionigenium modestum. GcdC shares less similarity with the carboxytransferase subunits of oxaloacetate decarboxylases from Klebsiella pneumoniae and Salmonella enterica enterica serovar Typhimurium.

Citations: [Bendrat93, Braune99]

Gene Citations: [Braune99]

Molecular Weight of Polypeptide: 13.908 kD (from nucleotide sequence), 24 kD (experimental)

Unification Links: ModBase:Q9ZAA7 , Protein Model Portal:Q9ZAA7 , Swiss-Model:Q9ZAA7 , UniProt:Q9ZAA7

Relationship Links: Entrez-Nucleotide:RELATED-TO:AF030576 , InterPro:IN-FAMILY:IPR000089 , InterPro:IN-FAMILY:IPR001882 , InterPro:IN-FAMILY:IPR011053 , Pfam:IN-FAMILY:PF00364 , Prosite:IN-FAMILY:PS00188 , Prosite:IN-FAMILY:PS50968

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Subunit of: glutaconyl-CoA decarboxylase

Species: Acidaminococcus fermentans

Subunit composition of glutaconyl-CoA decarboxylase = [GcdA]2[GcdB]2[GcdC]2[GcdD]2

Glutaconyl-CoA decarboxylase is a membrane bound, biotin-containing enzyme, which catalyzes the decarboxylation of (E)-glutaconyl-CoA to crotonyl-CoA. The enzyme uses the free energy of decarboxylation (δ Go' ~-30 kJ mol-1) to drive the active transport of sodium ions across the membrane [Bendrat93, Braune99].

Glutaconyl-CoA decarboxylases have also been purified from the Gram-positive bacteria Peptoniphilus asaccharolyticus and [Clostridium] symbiosum and the Gram-negative bacterium Fusobacterium nucleatum [Beatrix90].

Locations: plasma membrane

GO Terms:

Cellular Component: GO:0016020 - membrane

Enzymatic reaction of: glutaconyl-CoA decarboxylase

Synonyms: GCD

EC Number:

(E)-glutaconyl-CoA + H+ <=> CO2 + crotonyl-CoA

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

In Pathways: L-glutamate degradation V (via hydroxyglutarate)

Cofactors or Prosthetic Groups: biotin [Buckel83]


Beatrix90: Beatrix B, Bendrat K, Rospert S, Buckel W (1990). "The biotin-dependent sodium ion pump glutaconyl-CoA decarboxylase from Fusobacterium nucleatum (subsp. nucleatum). Comparison with the glutaconyl-CoA decarboxylases from gram-positive bacteria." Arch Microbiol 154(4);362-9. PMID: 2244788

Bendrat93: Bendrat K, Buckel W (1993). "Cloning, sequencing and expression of the gene encoding the carboxytransferase subunit of the biotin-dependent Na+ pump glutaconyl-CoA decarboxylase from Acidaminococcus fermentans in Escherichia coli." Eur J Biochem 1993;211(3);697-702. PMID: 8382157

Braune99: Braune A, Bendrat K, Rospert S, Buckel W (1999). "The sodium ion translocating glutaconyl-CoA decarboxylase from Acidaminococcus fermentans: cloning and function of the genes forming a second operon." Mol Microbiol 1999;31(2);473-87. PMID: 10027965

Buckel83: Buckel W, Semmler R (1983). "Purification, characterisation and reconstitution of glutaconyl-CoA decarboxylase, a biotin-dependent sodium pump from anaerobic bacteria." Eur J Biochem 1983;136(2);427-34. PMID: 6628393

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Jul 28, 2015, biocyc14.