MetaCyc Enzyme: nicotinamide phosphoribosyltransferase

Gene: nadV Accession Number: G-6261 (MetaCyc)

Species: Haemophilus ducreyi

The gene encoding the Haemophilus ducreyi nicotinamide phosphoribosyltransferase, nadV, was cloned from pNAD1, a plasmid that confers V-factor independence on that strain [Munson04, Martin01a]. The presence of this gene was shown to confer V-factor independence on other strains, including Haemophilus influenzae and Actinobacillus pleuropneumoniae [Martin01a].

While a similar gene has not been found in Escherichia coli [Kurnasov02], genes with significant sequence homology were found in many other organisms, including the human pre-B-cell colony enhancing factor PBEF and the bacterial species Mycoplasma genitalium, Mycoplasma pneumoniae, Shewanella putrefaciens, Synechocystis sp., Deinococcus radiodurans,Pasteurella multocida, and Aggregatibacter actinomycetemcomitans [Martin01a].

Eukaryotic organisms also poccess a similar gene. Preiss and Handler reported the isolation of such enzyme as early as 1956 [Preiss57].

Molecular Weight of Polypeptide: 55.619 kD (from nucleotide sequence)

Unification Links: Entrez-gene:2716561 , ModBase:Q9APM3 , Protein Model Portal:Q9APM3 , String:233412.HD1447 , Swiss-Model:Q9APM3 , UniProt:Q9APM3

Relationship Links: InterPro:IN-FAMILY:IPR002638 , InterPro:IN-FAMILY:IPR007229 , InterPro:IN-FAMILY:IPR016471 , Panther:IN-FAMILY:PTHR11098:SF2 , Pfam:IN-FAMILY:PF04095

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

MultiFun Terms: metabolism biosynthesis of building blocks cofactors, small molecule carriers nicotinamide adenine dinucleotide

Enzymatic reaction of: nicotinamide phosphoribosyltransferase

EC Number:

β-nicotinamide D-ribonucleotide + diphosphate <=> nicotinamide + 5-phospho-α-D-ribose 1-diphosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: NAD biosynthesis III


Kurnasov02: Kurnasov OV, Polanuyer BM, Ananta S, Sloutsky R, Tam A, Gerdes SY, Osterman AL (2002). "Ribosylnicotinamide kinase domain of NadR protein: identification and implications in NAD biosynthesis." J Bacteriol 184(24);6906-17. PMID: 12446641

Martin01a: Martin PR, Shea RJ, Mulks MH (2001). "Identification of a plasmid-encoded gene from Haemophilus ducreyi which confers NAD independence." J Bacteriol 183(4);1168-74. PMID: 11157928

Munson04: Munson RS, Zhong H, Mungur R, Ray WC, Shea RJ, Mahairas GG, Mulks MH (2004). "Haemophilus ducreyi strain ATCC 27722 contains a genetic element with homology to the vibrio RS1 element that can replicate as a plasmid and confer NAD independence on haemophilus influenzae." Infect Immun 72(2);1143-6. PMID: 14742562

Preiss57: Preiss, J., Handler, P. (1957). "Enzymatic synthesis of nicotinamide mononucleotide." J Biol Chem 225(2);759-70. PMID: 13416279

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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