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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: nicotinamide phosphoribosyltransferase

Gene: nadV Accession Number: G-6261 (MetaCyc)

Species: Haemophilus ducreyi

Summary:
The gene encoding the Haemophilus ducreyi nicotinamide phosphoribosyltransferase, nadV, was cloned from pNAD1, a plasmid that confers V-factor independence on that strain [Munson04, Martin01a]. The presence of this gene was shown to confer V-factor independence on other strains, including Haemophilus influenzae and Actinobacillus pleuropneumoniae [Martin01a].

While a similar gene has not been found in Escherichia coli [Kurnasov02], genes with significant sequence homology were found in many other organisms, including the human pre-B-cell colony enhancing factor PBEF and the bacterial species Mycoplasma genitalium, Mycoplasma pneumoniae, Shewanella putrefaciens, Synechocystis sp., Deinococcus radiodurans,Pasteurella multocida, and Aggregatibacter actinomycetemcomitans [Martin01a].

Eukaryotic organisms also poccess a similar gene. Preiss and Handler reported the isolation of such enzyme as early as 1956 [Preiss57].

Molecular Weight of Polypeptide: 55.619 kD (from nucleotide sequence)

Unification Links: Entrez-gene:2716561 , ModBase:Q9APM3 , Protein Model Portal:Q9APM3 , String:233412.HD1447 , Swiss-Model:Q9APM3 , UniProt:Q9APM3

Relationship Links: InterPro:IN-FAMILY:IPR002638 , InterPro:IN-FAMILY:IPR007229 , InterPro:IN-FAMILY:IPR016471 , Panther:IN-FAMILY:PTHR11098:SF2 , Pfam:IN-FAMILY:PF04095

Gene-Reaction Schematic: ?

MultiFun Terms: metabolism biosynthesis of building blocks cofactors, small molecule carriers nicotinamide adenine dinucleotide


Enzymatic reaction of: nicotinamide phosphoribosyltransferase

EC Number: 2.4.2.12

β-nicotinamide D-ribonucleotide + diphosphate <=> nicotinamide + 5-phospho-α-D-ribose 1-diphosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: NAD biosynthesis III


References

Kurnasov02: Kurnasov OV, Polanuyer BM, Ananta S, Sloutsky R, Tam A, Gerdes SY, Osterman AL (2002). "Ribosylnicotinamide kinase domain of NadR protein: identification and implications in NAD biosynthesis." J Bacteriol 184(24);6906-17. PMID: 12446641

Martin01a: Martin PR, Shea RJ, Mulks MH (2001). "Identification of a plasmid-encoded gene from Haemophilus ducreyi which confers NAD independence." J Bacteriol 183(4);1168-74. PMID: 11157928

Munson04: Munson RS, Zhong H, Mungur R, Ray WC, Shea RJ, Mahairas GG, Mulks MH (2004). "Haemophilus ducreyi strain ATCC 27722 contains a genetic element with homology to the vibrio RS1 element that can replicate as a plasmid and confer NAD independence on haemophilus influenzae." Infect Immun 72(2);1143-6. PMID: 14742562

Preiss57: Preiss, J., Handler, P. (1957). "Enzymatic synthesis of nicotinamide mononucleotide." J Biol Chem 225(2);759-70. PMID: 13416279


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, biocyc12.