|Gene:||nadV||Accession Number: G-6261 (MetaCyc)|
Species: Haemophilus ducreyi
The gene encoding the Haemophilus ducreyi nicotinamide phosphoribosyltransferase, nadV, was cloned from pNAD1, a plasmid that confers V-factor independence on that strain [Munson04, Martin01]. The presence of this gene was shown to confer V-factor independence on other strains, including Haemophilus influenzae and Actinobacillus pleuropneumoniae [Martin01].
While a similar gene has not been found in Escherichia coli [Kurnasov02], genes with significant sequence homology were found in many other organisms, including the human pre-B-cell colony enhancing factor PBEF and the bacterial species Mycoplasma genitalium, Mycoplasma pneumoniae, Shewanella putrefaciens, Synechocystis sp., Deinococcus radiodurans,Pasteurella multocida, and Aggregatibacter actinomycetemcomitans [Martin01].
Eukaryotic organisms also poccess a similar gene. Preiss and Handler reported the isolation of such enzyme as early as 1956 [Preiss57].
Molecular Weight of Polypeptide: 55.619 kD (from nucleotide sequence)
|MultiFun Terms:||metabolism → biosynthesis of building blocks → cofactors, small molecule carriers → nicotinamide adenine dinucleotide|
Enzymatic reaction of: nicotinamide phosphoribosyltransferase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the opposite direction.
In Pathways: NAD biosynthesis III
Kurnasov02: Kurnasov OV, Polanuyer BM, Ananta S, Sloutsky R, Tam A, Gerdes SY, Osterman AL (2002). "Ribosylnicotinamide kinase domain of NadR protein: identification and implications in NAD biosynthesis." J Bacteriol 184(24);6906-17. PMID: 12446641
Munson04: Munson RS, Zhong H, Mungur R, Ray WC, Shea RJ, Mahairas GG, Mulks MH (2004). "Haemophilus ducreyi strain ATCC 27722 contains a genetic element with homology to the vibrio RS1 element that can replicate as a plasmid and confer NAD independence on haemophilus influenzae." Infect Immun 72(2);1143-6. PMID: 14742562
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