|Gene:||iaaH||Accession Number: G-5841 (MetaCyc)|
Species: Pseudomonas savastanoi
Indoleacetamide hydrolase catalyzes the hydrolysis of indoleacetamide to indoleacetate and ammonia. This enzyme has been cloned from a strain of Pseudomonas savastanoi (strain 2009) that has been isolated from oleander plants in California, US. In this strain, the iaaH gene, which encodes this enzyme, is loacted on a 53 kb plasmid, named pIAA1 [Comai80], and is likely to be the last gene in the operon [Palm89]. As pathway end products had no effect on the activity of this enzyme, it is probable that this enzyme does not function in a regulatory capacity [Hutcheson85].
Gene Citations: [Yamada85]
Molecular Weight of Polypeptide: 48.578 kD (from nucleotide sequence), 47 kD (experimental) [Palm89 ]
Relationship Links: Entrez-Nucleotide:RELATED-TO:M11035 , InterPro:IN-FAMILY:IPR000120 , InterPro:IN-FAMILY:IPR020556 , InterPro:IN-FAMILY:IPR023631 , Panther:IN-FAMILY:PTHR11895 , Pfam:IN-FAMILY:PF01425 , Prosite:IN-FAMILY:PS00571
|MultiFun Terms:||metabolism → carbon utilization → amino acids|
Enzymatic reaction of: indoleacetamide hydrolase
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: indole-3-acetate biosynthesis III (bacteria)
Hutcheson85: Hutcheson SW, Kosuge T (1985). "Regulation of 3-indoleacetic acid production in Pseudomonas syringae pv. savastanoi. Purification and properties of tryptophan 2-monooxygenase." J Biol Chem 260(10);6281-7. PMID: 3997822
Yamada85: Yamada, T., Palm, C. J., Brooks, B., Kosuge, T. (1985). "Nucleotide sequences of the Pseudomonas savastanoi indoleacetic acid genes show homology with the Agrobacterium tumefaciens T-DNA." Proc. Natl. Acad. USA 82:6522-6526.
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