MetaCyc Enzyme: dolichol kinase

Gene: SEC59 Accession Number: G-5521 (MetaCyc)

Synonyms: dolichol phosphokinase

Species: Saccharomyces cerevisiae

The product of gene SEC59 was computationally predicted to be highly hydrophobic and to contain two potential N-glycosylation sites [Bernstein89]. The product of gene SEC59 contains a 13-amino acid consensus sequence thought to be involved in dolichol recognition. This sequence is also found in the glycosyltransferase products of genes ALG1, ALG7, and DPM1 [Albright89].

Human dolichol kinase has been cloned and expressed in Escherichia coli and its topological arrangement as a membrane protein characterized [Shridas06]. In humans, mutations in the gene encoding dolichol kinase can cause a lethal metabolic disorder [Kranz07].

Citations: [Heller92]

Gene Citations: [Heller92]

Locations: endoplasmic reticulum

Map Position: [295,178 <- 296,737]

Molecular Weight of Polypeptide: 58.916 kD (from nucleotide sequence)

Unification Links: DIP:DIP-8198N , Entrez-gene:855026 , ModBase:P20048 , Protein Model Portal:P20048 , String:4932.YMR013C , Swiss-Model:P20048 , UniProt:P20048

Relationship Links: InterPro:IN-FAMILY:IPR000374 , InterPro:IN-FAMILY:IPR026566 , Panther:IN-FAMILY:PTHR13205:SF14 , Pfam:IN-FAMILY:PF01148

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005783 - endoplasmic reticulum [Bernstein89]

Revised 21-Jan-2009 by Fulcher CA , SRI International

Enzymatic reaction of: dolichol kinase

EC Number:

a dolichol + CTP <=> a dolichyl phosphate + CDP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: dolichol and dolichyl phosphate biosynthesis

Saccharomyces cerevisiae cells carrying the SEC59 mutation are deficient in dolichol kinase activity [Heller92].


Albright89: Albright CF, Orlean P, Robbins PW (1989). "A 13-amino acid peptide in three yeast glycosyltransferases may be involved in dolichol recognition." Proc Natl Acad Sci U S A 86(19);7366-9. PMID: 2678101

Bernstein89: Bernstein M, Kepes F, Schekman R (1989). "Sec59 encodes a membrane protein required for core glycosylation in Saccharomyces cerevisiae." Mol Cell Biol 9(3);1191-9. PMID: 2657387

Heller92: Heller L, Orlean P, Adair WL (1992). "Saccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity." Proc Natl Acad Sci U S A 89(15);7013-6. PMID: 1323123

Kranz07: Kranz C, Jungeblut C, Denecke J, Erlekotte A, Sohlbach C, Debus V, Kehl HG, Harms E, Reith A, Reichel S, Grobe H, Hammersen G, Schwarzer U, Marquardt T (2007). "A defect in dolichol phosphate biosynthesis causes a new inherited disorder with death in early infancy." Am J Hum Genet 80(3);433-40. PMID: 17273964

Shridas06: Shridas P, Waechter CJ (2006). "Human dolichol kinase, a polytopic endoplasmic reticulum membrane protein with a cytoplasmically oriented CTP-binding site." J Biol Chem 281(42);31696-704. PMID: 16923818

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc12.