Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: dolichol kinase

Gene: SEC59 Accession Number: G-5521 (MetaCyc)

Synonyms: dolichol phosphokinase

Species: Saccharomyces cerevisiae

Summary:
The product of gene SEC59 was computationally predicted to be highly hydrophobic and to contain two potential N-glycosylation sites [Bernstein89]. The product of gene SEC59 contains a 13-amino acid consensus sequence thought to be involved in dolichol recognition. This sequence is also found in the glycosyltransferase products of genes ALG1, ALG7, and DPM1 [Albright89].

Human dolichol kinase has been cloned and expressed in Escherichia coli and its topological arrangement as a membrane protein characterized [Shridas06]. In humans, mutations in the gene encoding dolichol kinase can cause a lethal metabolic disorder [Kranz07].

Citations: [Heller92]

Gene Citations: [Heller92]

Locations: endoplasmic reticulum

Map Position: [295,178 <- 296,737]

Molecular Weight of Polypeptide: 58.916 kD (from nucleotide sequence)

Unification Links: DIP:DIP-8198N , Entrez-gene:855026 , ModBase:P20048 , Protein Model Portal:P20048 , String:4932.YMR013C , Swiss-Model:P20048 , UniProt:P20048

Relationship Links: InterPro:IN-FAMILY:IPR000374 , Pfam:IN-FAMILY:PF01148

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005783 - endoplasmic reticulum [Bernstein89]

Credits:
Revised 21-Jan-2009 by Fulcher CA , SRI International


Enzymatic reaction of: dolichol kinase

EC Number: 2.7.1.108

a dolichol + CTP <=> a dolichyl phosphate + CDP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: dolichol and dolichyl phosphate biosynthesis

Summary:
Saccharomyces cerevisiae cells carrying the SEC59 mutation are deficient in dolichol kinase activity [Heller92].


References

Albright89: Albright CF, Orlean P, Robbins PW (1989). "A 13-amino acid peptide in three yeast glycosyltransferases may be involved in dolichol recognition." Proc Natl Acad Sci U S A 86(19);7366-9. PMID: 2678101

Bernstein89: Bernstein M, Kepes F, Schekman R (1989). "Sec59 encodes a membrane protein required for core glycosylation in Saccharomyces cerevisiae." Mol Cell Biol 9(3);1191-9. PMID: 2657387

Heller92: Heller L, Orlean P, Adair WL (1992). "Saccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity." Proc Natl Acad Sci U S A 89(15);7013-6. PMID: 1323123

Kranz07: Kranz C, Jungeblut C, Denecke J, Erlekotte A, Sohlbach C, Debus V, Kehl HG, Harms E, Reith A, Reichel S, Grobe H, Hammersen G, Schwarzer U, Marquardt T (2007). "A defect in dolichol phosphate biosynthesis causes a new inherited disorder with death in early infancy." Am J Hum Genet 80(3);433-40. PMID: 17273964

Shridas06: Shridas P, Waechter CJ (2006). "Human dolichol kinase, a polytopic endoplasmic reticulum membrane protein with a cytoplasmically oriented CTP-binding site." J Biol Chem 281(42);31696-704. PMID: 16923818


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 20, 2014, BIOCYC13B.