|Gene:||cobF||Accession Number: G-499 (MetaCyc)|
Synonyms: precorrin 6A synthase
Species: Pseudomonas denitrificans
The Pseudomonas denitrificans enzyme precorrin-5 (C1)-methyltransferase catalyzes two reactions - the methylation of carbon C1 of precorrin-5, and its deacetylation, forming precorrin-6A. The cobF gene encoding the enzyme has been cloned and sequenced, and the enzyme, which requires S-adenosyl-L-methionine for activity, has been purified [Debussche93].
Gene Citations: [Crouzet90]
Molecular Weight of Polypeptide: 28.945 kD (from nucleotide sequence), 31 kD (experimental) [Debussche93 ]
Relationship Links: Entrez-Nucleotide:RELATED-TO:M59301 , InterPro:IN-FAMILY:IPR000878 , InterPro:IN-FAMILY:IPR012797 , InterPro:IN-FAMILY:IPR014776 , InterPro:IN-FAMILY:IPR014777 , Pfam:IN-FAMILY:PF00590
Enzymatic reaction of: precorrin-5 (C1)-methyltransferase
EC Number: 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
Crouzet90: Crouzet J, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Thibaut D, Debussche L (1990). "Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid." J Bacteriol 172(10);5980-90. PMID: 2211521
Debussche93: Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F (1993). "Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans." J Bacteriol 1993;175(22);7430-40. PMID: 8226690
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