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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Polypeptide: formyltransferase/hydrolase complex β subunit

Gene: fhcB Accession Number: G-3101 (MetaCyc)

Synonyms: orf2

Species: Methylobacterium extorquens AM1

Component of: formyltransferase/hydrolase complex (summary available)

Gene Citations: [Chistoserdova98]

Molecular Weight of Polypeptide: 37 kD (experimental) [Pomper01 ]

Unification Links: ModBase:Q9FA41 , Swiss-Model:Q9FA41 , UniProt:Q9FA41

Relationship Links: Entrez-Nucleotide:RELATED-TO:AF032114

Gene-Reaction Schematic: ?

MultiFun Terms: metabolism carbon utilization


Subunit of: formyltransferase/hydrolase complex

Species: Methylobacterium extorquens AM1

Subunit composition of formyltransferase/hydrolase complex = [FhcA]2[FhcB]2[FhcC]2[FhcD]2
         formyltransferase/hydrolase complex α subunit = FhcA
         formyltransferase/hydrolase complex β subunit = FhcB
         formyltransferase/hydrolase complex γ subunit = FhcC
         formyltransferase/hydrolase complex δ subunit = FhcD

Summary:
the Fhc (Ftr-hydroxylase complex) from M. extorquens is an octaheteromer, composed of 4 different subunits in 2:2:2:2 stoichiometry [Pomper01]. It catalyzes the transfer of a formyl group from N5-formyl-H4MPT to an analogue of MFR (methanofuran), and the hydrolysis of formyl-MFR to formate (the nature of the MFR analogue is still not known at this time) [Pomper02]. This is a key step in the pathway of H4MPT-dependent formaldehyde oxidation, resulting in formate which can be oxidized to CO2.

Locations: cytosol

Molecular Weight: 310 kD (experimental) [Pomper01]

GO Terms:

Cellular Component: GO:0005829 - cytosol [Pomper01]


Enzymatic reaction of: formyltransferase/hydrolase complex

5-formyl-tetrahydromethanopterin + H2O <=> formate + tetrahydromethanopterin + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is irreversible in the direction shown.

In Pathways: superpathway of C1 compounds oxidation to CO2 , formaldehyde oxidation V (H4MPT pathway)

Citations: [Chistoserdova03]

Activators (Unknown Mechanism): Na+ [Pomper01] , K+ [Pomper01]

Kinetic Parameters:

Substrate
Km (μM)
5-formyl-tetrahydromethanopterin
30.0

pH(opt): 7 [Pomper01]


References

Chistoserdova03: Chistoserdova L, Chen SW, Lapidus A, Lidstrom ME (2003). "Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view." J Bacteriol 185(10);2980-7. PMID: 12730156

Chistoserdova98: Chistoserdova L, Vorholt JA, Thauer RK, Lidstrom ME (1998). "C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea." Science 281(5373);99-102. PMID: 9651254

Pomper01: Pomper BK, Vorholt JA (2001). "Characterization of the formyltransferase from Methylobacterium extorquens AM1." Eur J Biochem 268(17);4769-75. PMID: 11532013

Pomper02: Pomper BK, Saurel O, Milon A, Vorholt JA (2002). "Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1." FEBS Lett 523(1-3);133-7. PMID: 12123819


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 20, 2014, BIOCYC13B.