MetaCyc Polypeptide: formyltransferase/hydrolase complex β subunit

Gene: fhcB Accession Number: G-3101 (MetaCyc)

Synonyms: orf2

Species: Methylobacterium extorquens AM1

Component of: formyltransferase/hydrolase complex (summary available)

Gene Citations: [Chistoserdova98]

Molecular Weight of Polypeptide: 37 kD (experimental) [Pomper01 ]

Unification Links: ModBase:Q9FA41 , Swiss-Model:Q9FA41 , UniProt:Q9FA41

Relationship Links: Entrez-Nucleotide:RELATED-TO:AF032114 , InterPro:IN-FAMILY:IPR029035

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

MultiFun Terms: metabolism carbon utilization

Subunit of: formyltransferase/hydrolase complex

Species: Methylobacterium extorquens AM1

Subunit composition of formyltransferase/hydrolase complex = [FhcA]2[FhcB]2[FhcC]2[FhcD]2
         formyltransferase/hydrolase complex α subunit = FhcA
         formyltransferase/hydrolase complex β subunit = FhcB
         formyltransferase/hydrolase complex γ subunit = FhcC
         formyltransferase/hydrolase complex δ subunit = FhcD

the Fhc (Ftr-hydroxylase complex) from M. extorquens is an octaheteromer, composed of 4 different subunits in 2:2:2:2 stoichiometry [Pomper01]. It catalyzes the transfer of a formyl group from N5-formyl-H4MPT to an analogue of MFR (methanofuran), and the hydrolysis of formyl-MFR to formate (the nature of the MFR analogue is still not known at this time) [Pomper02]. This is a key step in the pathway of H4MPT-dependent formaldehyde oxidation, resulting in formate which can be oxidized to CO2.

Locations: cytosol

Molecular Weight: 310 kD (experimental) [Pomper01]

GO Terms:

Cellular Component: GO:0005829 - cytosol [Pomper01]

Enzymatic reaction of: formyltransferase/hydrolase complex

5-formyl-tetrahydromethanopterin + H2O <=> formate + tetrahydromethanopterin + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is irreversible in the direction shown.

In Pathways: superpathway of C1 compounds oxidation to CO2 , formaldehyde oxidation V (H4MPT pathway)

Citations: [Chistoserdova03]

Activators (Unknown Mechanism): Na+ [Pomper01] , K+ [Pomper01]

Kinetic Parameters:

Km (μM)

pH(opt): 7 [Pomper01]


Chistoserdova03: Chistoserdova L, Chen SW, Lapidus A, Lidstrom ME (2003). "Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view." J Bacteriol 185(10);2980-7. PMID: 12730156

Chistoserdova98: Chistoserdova L, Vorholt JA, Thauer RK, Lidstrom ME (1998). "C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea." Science 281(5373);99-102. PMID: 9651254

Pomper01: Pomper BK, Vorholt JA (2001). "Characterization of the formyltransferase from Methylobacterium extorquens AM1." Eur J Biochem 268(17);4769-75. PMID: 11532013

Pomper02: Pomper BK, Saurel O, Milon A, Vorholt JA (2002). "Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1." FEBS Lett 523(1-3);133-7. PMID: 12123819

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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