MetaCyc Enzyme: sulfoacetaldehyde reductase

Gene: isfD Accession Number: G-12505 (MetaCyc)

Species: Klebsiella oxytoca TauN1

Taurine-grown cells of Klebsiella oxytoca TauN1 were found to contain an NADP+-coupled sulfoacetaldehyde reductase [Styp05]. The enzyme, which is inducible, is soluble, in contrast to the membrane-bound, cytochrome c-coupled enzyme involved in the dissimilation of taurine in Paracoccus denitrificans NKNIS [Bruggemann04] (see isethionate degradation).

THe enzyme was partially purified and characterized. It was found to be a multimer of 80 kDa, composed on subunits of 29 kDa. The N-terminal amino-acid sequence of the enzyme was determined, and matched to the partially sequenced genome.

Molecular Weight of Polypeptide: 27.296 kD (from nucleotide sequence), 29.0 kD (experimental) [Styp05 ]

Unification Links: Entrez-Nucleotide:FJ711704 , Protein Model Portal:D3U1D9 , UniProt:D3U1D9

Relationship Links: InterPro:IN-FAMILY:IPR002198 , InterPro:IN-FAMILY:IPR002347 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR020904 , Pfam:IN-FAMILY:PF00106 , Prints:IN-FAMILY:PR00080 , Prints:IN-FAMILY:PR00081 , Prosite:IN-FAMILY:PS00061

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 09-Feb-2011 by Caspi R , SRI International

Enzymatic reaction of: sulfoacetaldehyde reductase

EC Number:

isethionate + NADP+ <=> sulfoacetaldehyde + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: sulfoacetaldehyde degradation III

Kinetic Parameters:

Km (μM)

T(opt): 40 °C [Styp05]

pH(opt): 5-6 [Styp05]


Bruggemann04: Bruggemann C, Denger K, Cook AM, Ruff J (2004). "Enzymes and genes of taurine and isethionate dissimilation in Paracoccus denitrificans." Microbiology 150(Pt 4);805-16. PMID: 15073291

Styp05: Styp von Rekowski K, Denger K, Cook AM (2005). "Isethionate as a product from taurine during nitrogen-limited growth of Klebsiella oxytoca TauN1." Arch Microbiol 183(5);325-30. PMID: 15883781

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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