|Gene:||isfD||Accession Number: G-12505 (MetaCyc)|
Species: Klebsiella oxytoca TauN1
Taurine-grown cells of Klebsiella oxytoca TauN1 were found to contain an NADP+-coupled sulfoacetaldehyde reductase [Styp05]. The enzyme, which is inducible, is soluble, in contrast to the membrane-bound, cytochrome c-coupled enzyme involved in the dissimilation of taurine in Paracoccus denitrificans NKNIS [Bruggemann04] (see isethionate degradation).
THe enzyme was partially purified and characterized. It was found to be a multimer of 80 kDa, composed on subunits of 29 kDa. The N-terminal amino-acid sequence of the enzyme was determined, and matched to the partially sequenced genome.
Molecular Weight of Polypeptide: 27.296 kD (from nucleotide sequence), 29.0 kD (experimental) [Styp05 ]
Relationship Links: InterPro:IN-FAMILY:IPR002198 , InterPro:IN-FAMILY:IPR002347 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR020904 , Pfam:IN-FAMILY:PF00106 , Prints:IN-FAMILY:PR00080 , Prints:IN-FAMILY:PR00081 , Prosite:IN-FAMILY:PS00061
Enzymatic reaction of: sulfoacetaldehyde reductase
EC Number: 188.8.131.523
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the opposite direction.
In Pathways: sulfoacetaldehyde degradation III
T(opt): 40 °C [Styp05]
pH(opt): 5-6 [Styp05]
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