Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: thiocyanate forming protein

Gene: TFP Accession Number: G-12435 (MetaCyc)

Species: Lepidium sativum

Molecular Weight of Polypeptide: 37.3 kD (from nucleotide sequence)

Unification Links: UniProt:A1XLE2

Relationship Links: InterPro:IN-FAMILY:IPR006652 , InterPro:IN-FAMILY:IPR015916 , Pfam:IN-FAMILY:PF01344

Gene-Reaction Schematic: ?

Credits:
Created 11-Jan-2011 by Zhang P , TAIR


Enzymatic reaction of: thiocyanate forming protein

benzylglucosinolate aglycone <=> benzyl thiocyanate + sulfate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate breakdown (via thiocyanate-forming protein)

pH(opt): 9.5 [Burow07]


Enzymatic reaction of: thiocyanate forming protein

benzylglucosinolate aglycone <=> phenylacetonitrile + sulfate + S0

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate breakdown (via thiocyanate-forming protein)

Summary:
The L. sativum TFP does not only catalyze thiocyanate and simple nitrile formation from benzylglucosinolate, but also the formation of simple nitrile and epithionitrile formation from ectopically supplied 4-methylthiobutylglucosinolate and allylglucosinolate, respectively [Burow07]. The protein shares 63-68% amino acid sequence identity with known epithiospecifier proteins.

pH(opt): 9.5 [Burow07]


References

Burow07: Burow M, Bergner A, Gershenzon J, Wittstock U (2007). "Glucosinolate hydrolysis in Lepidium sativum--identification of the thiocyanate-forming protein." Plant Mol Biol 63(1);49-61. PMID: 17139450


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC13A.