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MetaCyc Enzyme: thiocyanate forming protein

Gene: TFP Accession Number: G-12435 (MetaCyc)

Species: Lepidium sativum

Molecular Weight of Polypeptide: 37.3 kD (from nucleotide sequence)

Unification Links: UniProt:A1XLE2

Relationship Links: InterPro:IN-FAMILY:IPR006652 , InterPro:IN-FAMILY:IPR015916 , Pfam:IN-FAMILY:PF01344

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 11-Jan-2011 by Zhang P , TAIR


Enzymatic reaction of: thiocyanate forming protein

benzylglucosinolate aglycone <=> benzyl thiocyanate + sulfate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate breakdown (via thiocyanate-forming protein)

pH(opt): 9.5 [Burow07]


Enzymatic reaction of: thiocyanate forming protein

benzylglucosinolate aglycone <=> S0 + phenylacetonitrile + sulfate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate breakdown (via thiocyanate-forming protein)

Summary:
The L. sativum TFP does not only catalyze thiocyanate and simple nitrile formation from benzylglucosinolate, but also the formation of simple nitrile and epithionitrile formation from ectopically supplied 4-methylthiobutylglucosinolate and allylglucosinolate, respectively [Burow07]. The protein shares 63-68% amino acid sequence identity with known epithiospecifier proteins.

pH(opt): 9.5 [Burow07]


References

Burow07: Burow M, Bergner A, Gershenzon J, Wittstock U (2007). "Glucosinolate hydrolysis in Lepidium sativum--identification of the thiocyanate-forming protein." Plant Mol Biol 63(1);49-61. PMID: 17139450


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat Apr 18, 2015, biocyc14.