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MetaCyc Enzyme: 1-hydroxy-carotenoid methylase

Gene: crtF Accession Number: G-12169 (MetaCyc)

Synonyms: demethylspheroidene and didehydrorhodopin O-methyltransferase

Species: Rubrivivax gelatinosus

Summary:
The subunit structure of this enzyme has not been reported.

Rubrivivax gelatinosus is a photosynthetic non-sulfur purple bacterium that produces carotenoids by both the spheroidene and spheroidenone biosynthesis and spirilloxanthin and 2,2'-diketo-spirilloxanthin biosynthesis pathways. These methylated carotenoids function as accessory pigments in the light-harvesting and reaction-center complexes of this β-proteobacterium. Insertional mutagenesis and carotenoid analysis studies confirmed the O-methyltransferase activity of CrtF in this organism. There was an absence of methoxy-carotenoids in the CrtF mutant [Pinta03].

The recombinant CrtF enzyme from the α-proteobacterium Rhodobacter capsulatus, a non-sulfur purple bacterium, has been expressed in Escherichia coli and characterized [Badenhop03] (see hydroxyneurosporene methyltransferase).

Molecular Weight of Polypeptide: 42.115 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q83YH7 , UniProt:Q83YH7

Relationship Links: Entrez-Nucleotide:PART-OF:AY234384 , InterPro:IN-FAMILY:IPR001077 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR016461 , InterPro:IN-FAMILY:IPR029063 , Pfam:IN-FAMILY:PF00891

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 07-Sep-2010 by Fulcher CA , SRI International


Enzymatic reaction of: demethylspheroidene methyltransferase (1-hydroxy-carotenoid methylase)

EC Number: 2.1.1.210

demethylspheroidene + S-adenosyl-L-methionine <=> spheroidene + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: spheroidene and spheroidenone biosynthesis


Enzymatic reaction of: hydroxyneurosporene methyltransferase (1-hydroxy-carotenoid methylase)

EC Number: 2.1.1.-

1-hydroxyneurosporene + S-adenosyl-L-methionine <=> methoxyneurosporene + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: spheroidene and spheroidenone biosynthesis


Enzymatic reaction of: hydroxyspirilloxanthin O-methyltransferase (1-hydroxy-carotenoid methylase)

EC Number: 2.1.1.-

hydroxyspirilloxanthin + S-adenosyl-L-methionine <=> spirilloxanthin + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: spirilloxanthin and 2,2'-diketo-spirilloxanthin biosynthesis

Summary:
This enzyme catalyzes the O-methylation of carotenoid C-1 hydroxyl groups using S-adenosyl-L-methionine as the methyl group donor (in [Pinta03]).


Enzymatic reaction of: 3,4-didehydrorhodopin O-methyltransferase (1-hydroxy-carotenoid methylase)

EC Number: 2.1.1.-

3,4-didehydrorhodopin + S-adenosyl-L-methionine <=> anhydrorhodovibrin + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: spirilloxanthin and 2,2'-diketo-spirilloxanthin biosynthesis

Summary:
This enzyme catalyzes the O-methylation of carotenoid C-1 hydroxyl groups using S-adenosyl-L-methionine as the methyl group donor (in [Pinta03]).


References

Badenhop03: Badenhop F, Steiger S, Sandmann M, Sandmann G (2003). "Expression and biochemical characterization of the 1-HO-carotenoid methylase CrtF from Rhodobacter capsulatus." FEMS Microbiol Lett 222(2);237-42. PMID: 12770713

Pinta03: Pinta V, Ouchane S, Picaud M, Takaichi S, Astier C, Reiss-Husson F (2003). "Characterization of unusual hydroxy- and ketocarotenoids in Rubrivivax gelatinosus: involvement of enzyme CrtF or CrtA." Arch Microbiol 179(5);354-62. PMID: 12664193


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat Mar 28, 2015, BIOCYC14B.